Natto beverage and preparation method thereof
A natto and beverage technology, which is applied in the field of preparation of the natto beverage, can solve problems such as difficulty in increasing the biomass of Bacillus natto, uneven fermentation degree of soybeans, poor product appearance and smell, etc., so as to prolong the shelf life of the product, Effect of elimination of health and safety hazards, improvement of appearance and smell
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Embodiment 1
[0052] The preparation of embodiment 1 women's health care natto liquid beverage
[0053] a. Rejuvenation and enrichment culture of Bacillus subtilis subsp. natto ODK-BN1 strain: Take 1 tube of Bacillus subtilis subsp. natto ODK-BN1 strain stored in deep-low temperature freezer, and add 2 times the dry weight of bacterial powder Quantity (w / v, g / mL) of normal saline dissolves bacterium powder, spreads on nutrient agar plate again (consists of the following components by mass volume percent concentration: 2% glucose, 0.5% beef extract, 1% peptone, 0.5% NaCl, 2% agarose, and the balance is water), cultivated at a temperature of 36°C for 24 hours to rejuvenate; pick a single colony on the nutrient agar plate, and inoculate it in the seed medium ( It consists of the following components in terms of mass volume percentage concentration: 2% glucose, 1% yeast extract, 1% peptone, 0.5% sodium chloride, 0.2% potassium dihydrogen phosphate, 0.4% hydrogen phosphate Dipotassium, 0.05% ma...
Embodiment 2
[0058] Embodiment 2 Preparation of Thrombolytic and Fat-reducing Natto Solid Beverage
[0059] A, the rejuvenation and enrichment culture of Bacillus subtilis subspecies natto ODK-BN1 bacterial strain: same as the method described in Example 1;
[0060] B, the preparation of liquid fermentation medium: the same as the method described in Example 1;
[0061] C, liquid submerged fermentation: the same as the method described in Example 1;
[0062] d. Preparation of natto beverage: freeze-dry the liquid fermentation product obtained in step c to obtain a freeze-dried powder. The freeze-drying conditions are: freezing temperature -50°C to -70°C, sublimation temperature 30°C, and drying time 72 hours; 5000g of the freeze-dried powder is mixed with an equal amount of cyclodextrin as a core material, suspended in hardened soybean oil with a melting point of 40-60°C to obtain a core material solution; % sodium alginate and 1% low-methyl pectin by mass volume concentration; the core ...
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