33 results about "Proteinase Gene" patented technology

The invention discloses streptomyces trypsin protein, heterologous expression carrier establishment and enzymatic properties of streptomyces trypsin, and belongs to the technical field of biology. Thefull-length gene GM2938 is obtained by digging whole-gene data of edophytic steptomyces A249, the full length of the gene is 759 bp, 253 amino acids are encoded, and the gene is expanded in vitro through a PCR. The heterologous cloning, expressing and purifying of the trypsin gene GM2938 in bacillus subtilis SCK6 are realized for the first time, and the representation of the enzymatic propertiesof the trypsin is conducted. The trypsin has the quite good enzymatic properties and has broad prospects in the leather process and food industrial application.

The invention discloses a cell model of HIV strain phenotypic drug resistance analysis and special pseudotype slow virus thereof; the invention constructs plasmid for expressing Gag protein of HIV, recombinant slow-virus plasmid for expressing reporter gene and helper plasmid for expressing Rev protein of HIV; under the effect of the helper plasmid pVSV-G, the three plasmid and HIV reverse transcriptase and the gene fragment of protease which are amplified from strain can obtain the pseudotype slow virus of the HIV reverse transcriptase and protease gene containing strain. The pseudotype slow virus is used for infecting the cells of mammals, thus obtaining the strain phenotypic resistance cell model based on the reporter gene. The cell model of the invention can carry out phenotypic resistance analysis to the strain, the reporter gene leads the cell model to have super high sensitivity, and the cell model of HIV strain phenotypic drug resistance analysis is applicable to the phenotypic drug resistance analysis of HIV strain in Chinese population.

Methods, systems, and computer readable media perform predictive modeling of gene activity. The methods may comprise obtaining the amino acid and / or nucleic acid sequence of a portion of the at least one gene from a biological sample obtained from a subject; comparing the amino acid and / or nucleic acid sequence of the portion of the at least one gene to a database of sequences for the portion of the at least one gene and for which the biological activity of the at least one gene has been evaluated; and applying a generalization of ridge regression analysis to estimate the effects of individual mutations in the at least one gene. A model is based on generalization of ridge regression (GRR) analysis to estimate the effects of individual mutations in at least one gene for the subject. At least one gene may comprise the reverse transcriptase and protease genes of an HIV vims.

The invention belongs to the technical field of agro-biology and particularly relates to fungus-originated acid protease Bs2688 and gene and application thereof. An amino acid sequence of the acid protease is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The invention provides a new protease gene; proteinases with good character are produced by means of genetic engineering; the new protease gene is applicable to the industries, such as feed, food and medicine.

The invention discloses a coxsackie virus B5 type virus-like particle as well as a preparation method and application thereof, and belongs to the technical field of gene engineering and biological medicines. The coxsackie virus B5 type virus-like particle is obtained by infecting Sf9 insect cells with recombinant baculovirus for expressing P1 capsid protein gene and 3CD protease gene of coxsackievirus B5 type, and culturing until more than 90% of the Sf9 insect cells are infected with lesions. The invention further discloses a preparation method and application of the coxsackie virus B5 typevirus-like particle. The coxsackie virus B5 type virus-like particle and a wild virus particle have similar appearance structures and sizes, the immunogenicity reaches or even exceeds the degree of the wild inactivated virus, and the coxsackie virus B5 type virus-like particle can be used for preparing a coxsackie virus B5 type virus-like particle vaccine.

The present invention relates to a swine vesicular disease virus-like particle prepared by a gene recombination technique, as well as a preparation method thereof. More particularly, the invention relates to a method for preparing a swine vesicular disease virus-like particle, wherein the structural protein precursor (Pl) gene and 3CD protease gene of swine vesicular disease virus are inserted simultaneously into a baculovirus expression vector and are expressed in insect cells, so that the expressed 3CD protease enzyme digests the structural protein precursor into individual proteins which then form the capsid of swine vesicular disease virus by an self-assembly process.

The invention discloses a phaseolus vulgaris metalloproteinase PvFtsH2 gene as well as an encoded protein and application thereof, relates to the field of gene engineering, and particularly relates toa plant metalloproteinase PvFtsH2 gene as well as an encoded protein and the application thereof. The invention provides a kidney bean FtsH metalloproteinase PvFtsH2 gene as well as an encoding protein and application thereof. A nucleotide sequence of the PvFtsH2 gene is shown as SEQ ID NO: 1 in a sequence table. The amino acid sequence of the encoded protein is shown as SEQ ID NO: 2. Phytophysiology and molecular biology methods prove that the PvFtsH2 gene plays a role in the photoinhibition response process, and mutation of the gene causes reduction of the FV / FM value, reduction of the chlorophyll content and D1 protein accumulation under the photoinhibition condition. The method has important application value in the aspect of increasing the yield of kidney beans by improving the lightenergy utilization rate.

The invention discloses a prokaryotic expression method for sea cucumber cathepsin, the prokaryotic expression method comprises the following steps of carrying out cloning on gene sequence of the sea cucumber cathepsin; constructing fusion expression vector with the gene sequence of the sea cucumber cathepsin on the basis of obtaining the sea cucumber cathepsin cloned gene sequence; transforming the fusion expression vector to expression host strain for inducing the expression of target protein. The prokaryotic expression method provided by the invention has the advantages that escherichia coli is adopted to express the sea cucumber cathepsin at low cost, and the quality of expression product is easy to control.

The invention discloses a blueberry salt-resistant drought-resistant gene VcLON2 and protein encoded by blueberry salt-resistant drought-resistant gene VcLON2 as well as application. VcLON2 has a sequence shown by SEQ ID NO.1 in a sequence list; by converting nicotiana benthamiana, the salt resistance and drought resistance of a transgenic plant can be obviously improved. The gene plays an important role in cultivating a salt-resistant and drought-resistant plant (especially a crop).

The invention relates to the field of genetic engineering and fermentation engineering, and provides a candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste. The candida utilis double-gene co-expression strain is constructed by integrating carboxypeptidase and endoprotease genes onto a candida utilis genome through a candida utilis expression vector. According to the candida utilis double-gene co-expression strain capable of degrading the kitchen waste, and the candida utilis double-gene co-expression strain can specifically degrade the protein components in the kitchen waste and decompose and convert the protein components into small peptides and amino acids.

The invention discloses a dsRNA (double-stranded ribonucleic acid) for inhibiting expression of wheat aphid serine proteinase I gene and application thereof. The invention provides a dsRNA molecule disclosed as Sequence 2 in the sequence table. The invention also provides a DNA (deoxyribonucleic acid) molecule for coding the RNA molecule. The invention also provides application of the RNA molecule or the DNA molecule, which is at least one of the following (1) to (4): (1) preventing and treating aphids; (2) promoting death of aphids; (3) inhibiting growth of aphids; and (4) inhibiting expression of the aphid in-vivo serine proteinase I gene. The dsRNA has important application value in preventing and treating aphids in agricultural production.

The invention provides a soybean type bacillus subtilis proteinase gene Gmsbtl.6-3. The soybean type bacillus subtilis proteinase gene Gmsbtl.6-3 is separated and cloned from a soybean root system mutant material M18, and a plant over-expression vector and an RNAi (RNA (Ribonucleic Acid) interfere) expression vector of the gene are constructed; soybean plants are converted and the converted plants have a phytophthora root rot resisting function; gene resources and basic materials are provided for culturing new varieties capable of resisting soybean phytophthora root rot.

The invention provides a construction method and application of a retinitis pigmentosa animal model. The invention specifically provides a mutant of a cathepsin D (Cathepsin D, CTSD) gene. The mutant is formed by repeating a C basic group at the 262nd site of the CTSD gene. The invention further provides a CTSD mutant protein. Compared with a wild type CTSD protein, the 88th amino acid of the mutant protein begins to be subjected to frameshift mutation. The invention also provides a nucleic acid molecule and a carrier for coding the mutant protein, and a pharmaceutical composition prepared from the mutant protein, the nucleic acid molecule and the carrier. When the pharmaceutical composition is applied to non-human mammals, retinitis pigmentosa diseases can be induced, so that the pharmaceutical composition can be used as a convenient and stable retinitis pigmentosa non-human mammal model.

The invention provides a method for improved high secretory production of proteins. The present invention addresses the problem of providing a production system which achieves, in a host cell such as yeast, high secretory production of proteins, particularly proteins having a complex structure such as an S-S bond and which is so safe as to obviate explosion-proof equipment and is suitable for industrial production. The present invention provides a transformed yeast obtained by introducing a chaperone gene and disrupting aox1 and / or protease genes, and a method for producing proteins using the transformed yeast.

The invention discloses a prokaryotic expression method of sea cucumber cathepsin. The method includes: cloning a gene sequence of the sea cucumber cathepsin; on the basis of acquiring a gene sequence clone of the sea cucumber cathepsin, constructing a fusion expression vector of the gene sequence of the sea cucumber cathepsin, and transforming the fusion expression vector to an expressing host cell so as to perform induced expression of a target protein. Therefore, the method has the advantages that the sea cucumber cathepsin is expressed with Escherichia coli at low cost and quality of an expressed product is easy to control.

A method for predicting the constitution susceptible to the onset of specific diseases in individual humans, for example, respiratory diseases such as chronic obstructive pulmonary diseases, sinobronchial syndrome, pulmonary emphysema, diffuse panbronchiolitis or bronchiectasis, effects of treatment on patients or prognosis of the treatment by analyzing the genetic polymorphisms of a human trypsin-like enzyme of the respiratory tract.

The invention provides an oryza sativa thioredoxin gene OsNDU, a protein, a vector, a host cell, a molecular marking method and an application of the molecular marking method. The OsNDU has a nucleotide sequence shown in SEQ ID No.1, a cDNA sequence of the OsNDU is shown in SEQ ID No.2, and a protein sequence of the OsNDU is shown in SEQ ID No.3. A scheme provided by the invention is a good example that thioredoxin participates in nilaparvata lugens resistance of oryza sativa, and the scheme has a certain reference value for understanding a function of thioredoxin and regulating and controlling resistance. A research of the OsNDU gene provides a good theoretical basis for a downstream molecular mechanism of nilaparvata lugens resistant genes of oryza sativa, and has reference significancefor researching a gene molecular function and breeding.

The invention belongs to the technical field of genetic engineering, and in particular relates to an aspergillus oryzae keratinase gene and its expression vector and application. The specific technical scheme is: a keratinase expression gene, the DNA sequence of which is shown in SEQ ID No:1. The gene expresses a keratinase, the amino acid sequence of which is shown in SEQ ID No:2. The invention provides a new gene resource capable of expressing keratinase, combines the gene with a plasmid, and transfers the gene into a bacterial strain to produce keratinase, thereby enriching the source of keratinase.

The invention provides rice thioredoxinase gene OsNDU, protein, carrier, host cell, molecular marker method and application. OsNDU has the nucleotide sequence shown in SEQ ID No.1, its cDNA sequence is shown in SEQ ID No.2, and its protein sequence is shown in SEQ ID No.3. The scheme of the present invention is a good example of the participation of thioredoxin enzyme in rice resistance to brown planthopper, which has certain reference value for understanding the function of thioredoxin enzyme and the regulation of resistance. The study of OsNDU gene provides a good theoretical basis for the downstream molecular mechanism of rice resistance to brown planthopper genes, and has reference significance for the study of gene molecular functions and breeding.

The bean metalloprotease PvFtsH2 gene and its encoded protein and application relate to the field of genetic engineering, in particular to a plant metalloprotease PvFtsH2 gene and its encoded protein and application. The invention provides a bean FtsH metalloprotease PvFtsH2 gene, its encoded protein and application. The nucleotide sequence of the PvFtsH2 gene is shown in SEQ ID NO: 1 in the sequence listing. The amino acid sequence of the encoded protein is shown in SEQ ID NO:2. The present invention confirms that the PvFtsH2 gene plays a role in the light-inhibition response process through plant physiology and molecular biology methods, and the mutation of the gene leads to F under light-inhibition conditions. V / F M The value decreased, the chlorophyll content decreased, and the D1 protein accumulated. The invention has important application value in increasing the yield of kidney beans by improving the utilization rate of light energy.

The invention belongs to the technical field of molecular biology, and in particular relates to a method for highly expressing recombinant human fibroblast growth factor 21 (rhFGF21) gene by using bacillus subtilis. First, the present invention adjusts the transcription and translation efficiency of the target protein gene by adding different types of cistron cistron sequences at the 5' end of the target protein gene, thereby optimizing its soluble expression level, which can be applied to optimize the expression of different types of heterologous proteins . Secondly, the present invention also optimizes the disulfide bond-related genes to construct the rhFGF21 disulfide bond folding efficiency; overexpresses the molecular chaperone system to construct the protein folding efficiency suitable for rhFGF21; The stable Bacillus subtilis genetically engineered strain finally provides a kind of Bacillus subtilis genetically engineered strain capable of efficiently secreting and expressing rhFGF21 protein with good application prospects.

The invention proposes a multiple primer, a kit and a detection method for detecting fermentation conditions that affect the expression of beer yeast protease A gene, which belong to the field of food detection and can solve the traditional problem of detecting protease A that affects beer yeast protease A by measuring the activity of protease A. The fermentation conditions of gene expression amount have the problems of long time-consuming, cumbersome detection process and long detection cycle. The technical scheme includes the design of multiple primers, reverse transcription and synthetic cDNA template using the total RNA of yeast strains extracted under different fermentation conditions as a template, multiple PCR amplification reactions, capillary electrophoresis separation, and the use of GeXP system for capillary electrophoresis separation. The results were analyzed; using the internal reference gene as a control, the expression level of the protease A gene was calculated in combination with the peak area of ​​the corresponding peak of the key gene, and the fermentation conditions affecting the expression level of the protease A gene in S. cerevisiae were judged according to the expression level of the protease A gene.

The invention discloses a monoclonal cell strain stably expressing a serine protease gene, a preparation method of the monoclonal cell strain, a kit containing the monoclonal cell strain, and application of the monoclonal cell strain. The monoclonal cell strain stably expressing the serine protease gene is cultured in a culture plate, and cell culture liquid is removed; classical swine fever virusraw liquid is subjected to doubling dilution, and the diluted virus liquid is added into the culture plate with the cell culture liquid being removed; and culture continues, a classical swine fever virus TCID50 is judged through a CPE phenomenon, and the TCID50 is calculated through a Reed-Muench method. The advantages that the monoclonal cell strain is used for visual detection of the classicalswine fever virus, the classical swine fever virus TCID50 is detected through the detection kit, a fluorescent microscope and a classical swine fever detection antibody are not needed, result judgement of the classical swine fever virus TCID50 is simple, and experimental repeatability is good are achieved.

The invention discloses an artificially-synthesized LSL gene for encoding a laetiporus sulphureus mushroom lectin N-acetyllactosamine binding domain and an expression vector and host cell containing the LSL gene. The nucleotide sequence of the LSL gene is SEQ ID NO. 1. The invention further discloses a target protein expression with LSL used as the fusion tag and a purification method thereof. The method specifically relates to LSL gene synthesis, construction of the expression vector containing the LSL gene and the target protein gene, construction of the expression vector containing the LSL gene and a protease gene, expression and purification of fusion protein, LSL protein tag removal, target protein purification and the like. The method is simple and easy to implement, one-step purification of target protein is achieved, high-purity target protein can be obtained, the protein purification cost is lowered, the method can be widely applied to activity protein in the fields of biological medicine, veterinary medicine and the like and large-scale preparation of vaccine antigen in the industry of biological products, and high practical value is achieved.

The invention belongs to the technical field of gene engineering, and particularly relates to an aspergillus oryzae keratinase gene and an expression vector and application thereof. According to the specific technical scheme, the keratinase expression gene has a DNA sequence shown as SEQ ID No: 1. The gene expresses keratinase having an amino acid sequence shown as SEQ ID No: 2. The invention provides a new gene resource capable of expressing keratinase, and the gene is bound with plasmids and transferred into a strain to produce keratinase, so that the source of keratinase is enriched.

The invention discloses a gene GmFtsH25 for encoding soybean FtsH metalloproteinase and an application of the gene GmFtsH25. The nucleotide sequence of an encoding region (CDS) of the GmFtsH25 is as shown in SEQ ID NO.1, the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and a GmFtsH25 overexpression transgenic plant is created by using an agrobacterium tumefaciens-mediated soybean cotyledonary node transformation method. The invention discloses the application of the gene GmFtsH25 for encoding soybean FtsH metalloproteinase, and it is found that compared with the net photosynthetic rate of a control plant, the net photosynthetic rate of a transgenic plant can be obviously improved by over-expression of the gene. Therefore, the gene disclosed by the invention can be introduced into a plant as a target gene to regulate the photosynthesis capacity of the transgenic plant, and is of great significance to the cultivation of a new variety of high-photosynthetic-efficiency soybeans.