39 results about "Proteinase Gene" patented technology

According to the present invention, a technique which enables the production of an antimicrobial peptide Persulcatusin at reduced cost has been developed. A plant cell into which a polynucleotide sequence that encodes a gene for a protease to which a signal peptide localized in an intercellular organelle is added and a polynucleotide sequence that encodes a Persulcatusin fusion protein to which a signal peptide localized in an intracellular organelle different from the aforementioned intracellular organelle is added are introduced is produced, wherein, in the Persulcatusin fusion protein, a sequence that is cleavable with the protease is disposed between Persulcatusin and a protein that fuses to the Persulcatusin. Thus, Persulcatusin, which can serve as a therapeutic agent for bovine mastitis, can be produced at low cost.

The present invention relates to a new therapeutic approach for treatment of ANCA-associated vasculitis (Anti-neutrophil cytoplasmic autoantibody-associated vasculitis, AAV). It provides an agent for reducing expression of proteinase 3 (PR3) in a cell, in particular, a gene editing compound targeting the proteinase 3 gene, an epigenetic editing compound targeting the proteinase 3 gene, or an expression vector encoding an inhibitory oligonucleotide capable of selectively binding to a target nucleic acid sequence in mRNA encoding proteinase 3, e.g., an inhibitory oligonucleotide capable of inducing RNAi or an antisense oligonucleotide. Further, a method of reducing expression of proteinase 3 in a hematopoietic stem cell or cell derived therefrom, e.g., a neutrophil, is provided, as well as such cells having a reduced expression of proteinase 3. The cells or agents can be used for treating a subject having a proteinase 3-ANCA-associated vasculitis.

In order to solve the technical problem of high-efficiency expression of CLMP protein in tumors in the prior art, the invention creatively provides a clostridium butyricum engineering strain. According to the engineering bacterial strain, ATCC19398 clostridium butyricum is used as a chassis bacterial strain, and the engineering bacterial strain comprises a recombinant expression vector for expressing CLMP protein. The inventor accidentally finds that the clostridium butyricum as a chassis strain can realize expression of CLMP in tumor cells, the technical problem of expression reduction of CLMP protein in colorectal cancer is fundamentally solved, and the clostridium butyricum has important significance in treatment of colorectal cancer. On the basis, the inventor knockout a purine synthesis related gene purM in the clostridium butyricum to realize the enrichment of the clostridium butyricum in tumors; further, by knocking out the protease gene lon, the expression quantity of CLMP protein is remarkably increased while precise positioning aiming at the tumor is met, so that the inhibition effect on the tumor is further improved. Moreover, the engineering strain can significantly inhibit the growth of tumors in a mouse model, is good in safety, provides a new idea for the treatment of colorectal cancer, and has important practical application value.

This invention belongs to the field of agricultural biotechnology, specifically relating to a fungal-derived acidic protease Bs2688, its gene, and its applications. The amino acid sequence of the protease of this invention is shown in SEQ ID NO.1 or SEQ ID NO.2. This invention provides a novel protease gene, enabling the production of a high-quality protease using genetic engineering techniques, which can be applied in industries such as feed, food, and medicine.

The invention discloses a keratinase gene as well as recombinant bacteria and application thereof. Relates to the technical field of gene engineering. The invention provides a gene, an enzyme, an expression vector, a recombinant bacterium and application thereof. The activity of the keratinase of the obtained strain is screened to obtain the keratin-producing recombinant pichia pastoris engineering strain. The engineering strain constructed by the method is high in keratinase activity and good in stability.