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33 results about "Proteinase Gene" patented technology

Novel streptomyces trypsin GM2938 and heterologous expression thereof

The invention discloses streptomyces trypsin protein, heterologous expression carrier establishment and enzymatic properties of streptomyces trypsin, and belongs to the technical field of biology. Thefull-length gene GM2938 is obtained by digging whole-gene data of edophytic steptomyces A249, the full length of the gene is 759 bp, 253 amino acids are encoded, and the gene is expanded in vitro through a PCR. The heterologous cloning, expressing and purifying of the trypsin gene GM2938 in bacillus subtilis SCK6 are realized for the first time, and the representation of the enzymatic propertiesof the trypsin is conducted. The trypsin has the quite good enzymatic properties and has broad prospects in the leather process and food industrial application.
Owner:SICHUAN UNIV

HIV stain drug-resistant phenotype analytical cell model and special pseudotype lentivirus therefor

InactiveCN101353667ARapid Phenotypic Resistance AnalysisSafety Phenotypic Resistance AnalysisMicrobiological testing/measurementViruses/bacteriophagesPhenotypic resistanceReverse transcriptase
The invention discloses a cell model of HIV strain phenotypic drug resistance analysis and special pseudotype slow virus thereof; the invention constructs plasmid for expressing Gag protein of HIV, recombinant slow-virus plasmid for expressing reporter gene and helper plasmid for expressing Rev protein of HIV; under the effect of the helper plasmid pVSV-G, the three plasmid and HIV reverse transcriptase and the gene fragment of protease which are amplified from strain can obtain the pseudotype slow virus of the HIV reverse transcriptase and protease gene containing strain. The pseudotype slow virus is used for infecting the cells of mammals, thus obtaining the strain phenotypic resistance cell model based on the reporter gene. The cell model of the invention can carry out phenotypic resistance analysis to the strain, the reporter gene leads the cell model to have super high sensitivity, and the cell model of HIV strain phenotypic drug resistance analysis is applicable to the phenotypic drug resistance analysis of HIV strain in Chinese population.
Owner:MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI

Methods and systems for predictive modeling of hiv-1 replication capacity

Methods, systems, and computer readable media perform predictive modeling of gene activity. The methods may comprise obtaining the amino acid and / or nucleic acid sequence of a portion of the at least one gene from a biological sample obtained from a subject; comparing the amino acid and / or nucleic acid sequence of the portion of the at least one gene to a database of sequences for the portion of the at least one gene and for which the biological activity of the at least one gene has been evaluated; and applying a generalization of ridge regression analysis to estimate the effects of individual mutations in the at least one gene. A model is based on generalization of ridge regression (GRR) analysis to estimate the effects of individual mutations in at least one gene for the subject. At least one gene may comprise the reverse transcriptase and protease genes of an HIV vims.
Owner:LAB OF AMERICA HLDG

acid protease Bs2688 and gene and application thereof

The invention belongs to the technical field of agro-biology and particularly relates to fungus-originated acid protease Bs2688 and gene and application thereof. An amino acid sequence of the acid protease is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The invention provides a new protease gene; proteinases with good character are produced by means of genetic engineering; the new protease gene is applicable to the industries, such as feed, food and medicine.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

Coxsackie virus B5 type virus-like particle as well as preparation method and application thereof

The invention discloses a coxsackie virus B5 type virus-like particle as well as a preparation method and application thereof, and belongs to the technical field of gene engineering and biological medicines. The coxsackie virus B5 type virus-like particle is obtained by infecting Sf9 insect cells with recombinant baculovirus for expressing P1 capsid protein gene and 3CD protease gene of coxsackievirus B5 type, and culturing until more than 90% of the Sf9 insect cells are infected with lesions. The invention further discloses a preparation method and application of the coxsackie virus B5 typevirus-like particle. The coxsackie virus B5 type virus-like particle and a wild virus particle have similar appearance structures and sizes, the immunogenicity reaches or even exceeds the degree of the wild inactivated virus, and the coxsackie virus B5 type virus-like particle can be used for preparing a coxsackie virus B5 type virus-like particle vaccine.
Owner:GUILIN MEDICAL UNIVERSITY

Construct of swine vesicular disease virus like particle using genetic engineering and the method for manufacturing thereof

The present invention relates to a swine vesicular disease virus-like particle prepared by a gene recombination technique, as well as a preparation method thereof. More particularly, the invention relates to a method for preparing a swine vesicular disease virus-like particle, wherein the structural protein precursor (Pl) gene and 3CD protease gene of swine vesicular disease virus are inserted simultaneously into a baculovirus expression vector and are expressed in insect cells, so that the expressed 3CD protease enzyme digests the structural protein precursor into individual proteins which then form the capsid of swine vesicular disease virus by an self-assembly process.
Owner:大韩民国

Phaseolus vulgaris metalloproteinase PvFtsH2 gene as well as encoding protein and application thereof

The invention discloses a phaseolus vulgaris metalloproteinase PvFtsH2 gene as well as an encoded protein and application thereof, relates to the field of gene engineering, and particularly relates toa plant metalloproteinase PvFtsH2 gene as well as an encoded protein and the application thereof. The invention provides a kidney bean FtsH metalloproteinase PvFtsH2 gene as well as an encoding protein and application thereof. A nucleotide sequence of the PvFtsH2 gene is shown as SEQ ID NO: 1 in a sequence table. The amino acid sequence of the encoded protein is shown as SEQ ID NO: 2. Phytophysiology and molecular biology methods prove that the PvFtsH2 gene plays a role in the photoinhibition response process, and mutation of the gene causes reduction of the FV / FM value, reduction of the chlorophyll content and D1 protein accumulation under the photoinhibition condition. The method has important application value in the aspect of increasing the yield of kidney beans by improving the lightenergy utilization rate.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Prokaryotic expression method for sea cucumber cathepsin

The invention discloses a prokaryotic expression method for sea cucumber cathepsin, the prokaryotic expression method comprises the following steps of carrying out cloning on gene sequence of the sea cucumber cathepsin; constructing fusion expression vector with the gene sequence of the sea cucumber cathepsin on the basis of obtaining the sea cucumber cathepsin cloned gene sequence; transforming the fusion expression vector to expression host strain for inducing the expression of target protein. The prokaryotic expression method provided by the invention has the advantages that escherichia coli is adopted to express the sea cucumber cathepsin at low cost, and the quality of expression product is easy to control.
Owner:ZHEJIANG OCEAN UNIV

Blueberry salt-resistant drought-resistant gene VcLON2 and protein encoded by blueberry salt-resistant drought-resistant gene VcLON2 as well as application

The invention discloses a blueberry salt-resistant drought-resistant gene VcLON2 and protein encoded by blueberry salt-resistant drought-resistant gene VcLON2 as well as application. VcLON2 has a sequence shown by SEQ ID NO.1 in a sequence list; by converting nicotiana benthamiana, the salt resistance and drought resistance of a transgenic plant can be obviously improved. The gene plays an important role in cultivating a salt-resistant and drought-resistant plant (especially a crop).
Owner:ZHEJIANG NORMAL UNIVERSITY

dsRNA (double-stranded ribonucleic acid) for inhibiting expression of wheat aphid serine proteinase i gene and application thereof

The invention discloses a dsRNA (double-stranded ribonucleic acid) for inhibiting expression of wheat aphid serine proteinase I gene and application thereof. The invention provides a dsRNA molecule disclosed as Sequence 2 in the sequence table. The invention also provides a DNA (deoxyribonucleic acid) molecule for coding the RNA molecule. The invention also provides application of the RNA molecule or the DNA molecule, which is at least one of the following (1) to (4): (1) preventing and treating aphids; (2) promoting death of aphids; (3) inhibiting growth of aphids; and (4) inhibiting expression of the aphid in-vivo serine proteinase I gene. The dsRNA has important application value in preventing and treating aphids in agricultural production.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI

Soybean type bacillus subtilis proteinase gene and application

The invention provides a soybean type bacillus subtilis proteinase gene Gmsbtl.6-3. The soybean type bacillus subtilis proteinase gene Gmsbtl.6-3 is separated and cloned from a soybean root system mutant material M18, and a plant over-expression vector and an RNAi (RNA (Ribonucleic Acid) interfere) expression vector of the gene are constructed; soybean plants are converted and the converted plants have a phytophthora root rot resisting function; gene resources and basic materials are provided for culturing new varieties capable of resisting soybean phytophthora root rot.
Owner:JILIN AGRICULTURAL UNIV

Construction method and application of retinitis pigmentosa animal model

The invention provides a construction method and application of a retinitis pigmentosa animal model. The invention specifically provides a mutant of a cathepsin D (Cathepsin D, CTSD) gene. The mutant is formed by repeating a C basic group at the 262nd site of the CTSD gene. The invention further provides a CTSD mutant protein. Compared with a wild type CTSD protein, the 88th amino acid of the mutant protein begins to be subjected to frameshift mutation. The invention also provides a nucleic acid molecule and a carrier for coding the mutant protein, and a pharmaceutical composition prepared from the mutant protein, the nucleic acid molecule and the carrier. When the pharmaceutical composition is applied to non-human mammals, retinitis pigmentosa diseases can be induced, so that the pharmaceutical composition can be used as a convenient and stable retinitis pigmentosa non-human mammal model.
Owner:SHANGHAI FIRST PEOPLES HOSPITAL

Prokaryotic expression method of sea cucumber cathepsin

The invention discloses a prokaryotic expression method of sea cucumber cathepsin. The method includes: cloning a gene sequence of the sea cucumber cathepsin; on the basis of acquiring a gene sequence clone of the sea cucumber cathepsin, constructing a fusion expression vector of the gene sequence of the sea cucumber cathepsin, and transforming the fusion expression vector to an expressing host cell so as to perform induced expression of a target protein. Therefore, the method has the advantages that the sea cucumber cathepsin is expressed with Escherichia coli at low cost and quality of an expressed product is easy to control.
Owner:OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV

Oryza sativa thioredoxin gene OsNDU, protein, vector, host cell, molecular marking method and application of molecular marking method

The invention provides an oryza sativa thioredoxin gene OsNDU, a protein, a vector, a host cell, a molecular marking method and an application of the molecular marking method. The OsNDU has a nucleotide sequence shown in SEQ ID No.1, a cDNA sequence of the OsNDU is shown in SEQ ID No.2, and a protein sequence of the OsNDU is shown in SEQ ID No.3. A scheme provided by the invention is a good example that thioredoxin participates in nilaparvata lugens resistance of oryza sativa, and the scheme has a certain reference value for understanding a function of thioredoxin and regulating and controlling resistance. A research of the OsNDU gene provides a good theoretical basis for a downstream molecular mechanism of nilaparvata lugens resistant genes of oryza sativa, and has reference significancefor researching a gene molecular function and breeding.
Owner:WUHAN UNIV +1

Multiple primers, kit and detection method for detecting fermentation conditions affecting the expression level of Saccharomyces cerevisiae protease a gene

The invention proposes a multiple primer, a kit and a detection method for detecting fermentation conditions that affect the expression of beer yeast protease A gene, which belong to the field of food detection and can solve the traditional problem of detecting protease A that affects beer yeast protease A by measuring the activity of protease A. The fermentation conditions of gene expression amount have the problems of long time-consuming, cumbersome detection process and long detection cycle. The technical scheme includes the design of multiple primers, reverse transcription and synthetic cDNA template using the total RNA of yeast strains extracted under different fermentation conditions as a template, multiple PCR amplification reactions, capillary electrophoresis separation, and the use of GeXP system for capillary electrophoresis separation. The results were analyzed; using the internal reference gene as a control, the expression level of the protease A gene was calculated in combination with the peak area of ​​the corresponding peak of the key gene, and the fermentation conditions affecting the expression level of the protease A gene in S. cerevisiae were judged according to the expression level of the protease A gene.
Owner:TSINGTAO BREWERY

Aspergillus oryzae keratinase gene and expression vector and application thereof

The invention belongs to the technical field of gene engineering, and particularly relates to an aspergillus oryzae keratinase gene and an expression vector and application thereof. According to the specific technical scheme, the keratinase expression gene has a DNA sequence shown as SEQ ID No: 1. The gene expresses keratinase having an amino acid sequence shown as SEQ ID No: 2. The invention provides a new gene resource capable of expressing keratinase, and the gene is bound with plasmids and transferred into a strain to produce keratinase, so that the source of keratinase is enriched.
Owner:HUAZHONG AGRI UNIV

Gene GmFtsH25 for encoding soybean FtsH metalloproteinase and application of gene GmFtsH25

The invention discloses a gene GmFtsH25 for encoding soybean FtsH metalloproteinase and an application of the gene GmFtsH25. The nucleotide sequence of an encoding region (CDS) of the GmFtsH25 is as shown in SEQ ID NO.1, the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and a GmFtsH25 overexpression transgenic plant is created by using an agrobacterium tumefaciens-mediated soybean cotyledonary node transformation method. The invention discloses the application of the gene GmFtsH25 for encoding soybean FtsH metalloproteinase, and it is found that compared with the net photosynthetic rate of a control plant, the net photosynthetic rate of a transgenic plant can be obviously improved by over-expression of the gene. Therefore, the gene disclosed by the invention can be introduced into a plant as a target gene to regulate the photosynthesis capacity of the transgenic plant, and is of great significance to the cultivation of a new variety of high-photosynthetic-efficiency soybeans.
Owner:NANJING AGRICULTURAL UNIVERSITY
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