33 results about "Proteinase Gene" patented technology

The invention relates to the field of genetic engineering and fermentation engineering, and provides a candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste. The candida utilis double-gene co-expression strain is constructed by integrating carboxypeptidase and endoprotease genes onto a candida utilis genome through a candida utilis expression vector. According to the candida utilis double-gene co-expression strain capable of degrading the kitchen waste, and the candida utilis double-gene co-expression strain can specifically degrade the protein components in the kitchen waste and decompose and convert the protein components into small peptides and amino acids.

The invention provides rice thioredoxinase gene OsNDU, protein, carrier, host cell, molecular marker method and application. OsNDU has the nucleotide sequence shown in SEQ ID No.1, its cDNA sequence is shown in SEQ ID No.2, and its protein sequence is shown in SEQ ID No.3. The scheme of the present invention is a good example of the participation of thioredoxin enzyme in rice resistance to brown planthopper, which has certain reference value for understanding the function of thioredoxin enzyme and the regulation of resistance. The study of OsNDU gene provides a good theoretical basis for the downstream molecular mechanism of rice resistance to brown planthopper genes, and has reference significance for the study of gene molecular functions and breeding.

A cathepsin B gene of bollworm and its clone and recombinant expression process are disclosed. Its preparing steps include extracting overall RNA from bollworm, reverse transcription to synthesize cDNA, designing primer, polymerase chain reaction, multiplication purifying, cloning it to pGEM-T Easy vector, transferring DH5 alpha cells for sequence test, fast multiplication to obtain end-to-end CDNA (1310 bp), and expressing proteinase in prokaryotic and eukaryotic organisms to generate cathepsin B with hydrolysis activity.

The invention proposes a multiple primer, a kit and a detection method for detecting fermentation conditions that affect the expression of beer yeast protease A gene, which belong to the field of food detection and can solve the traditional problem of detecting protease A that affects beer yeast protease A by measuring the activity of protease A. The fermentation conditions of gene expression amount have the problems of long time-consuming, cumbersome detection process and long detection cycle. The technical scheme includes the design of multiple primers, reverse transcription and synthetic cDNA template using the total RNA of yeast strains extracted under different fermentation conditions as a template, multiple PCR amplification reactions, capillary electrophoresis separation, and the use of GeXP system for capillary electrophoresis separation. The results were analyzed; using the internal reference gene as a control, the expression level of the protease A gene was calculated in combination with the peak area of ​​the corresponding peak of the key gene, and the fermentation conditions affecting the expression level of the protease A gene in S. cerevisiae were judged according to the expression level of the protease A gene.

The invention discloses a monoclonal cell strain stably expressing a serine protease gene, a preparation method of the monoclonal cell strain, a kit containing the monoclonal cell strain, and application of the monoclonal cell strain. The monoclonal cell strain stably expressing the serine protease gene is cultured in a culture plate, and cell culture liquid is removed; classical swine fever virusraw liquid is subjected to doubling dilution, and the diluted virus liquid is added into the culture plate with the cell culture liquid being removed; and culture continues, a classical swine fever virus TCID50 is judged through a CPE phenomenon, and the TCID50 is calculated through a Reed-Muench method. The advantages that the monoclonal cell strain is used for visual detection of the classicalswine fever virus, the classical swine fever virus TCID50 is detected through the detection kit, a fluorescent microscope and a classical swine fever detection antibody are not needed, result judgement of the classical swine fever virus TCID50 is simple, and experimental repeatability is good are achieved.

The invention provides soybean subtilisin gene Gmsbt1.6-3, which is the soybean subtilisin gene Gmsbt1.6-3 isolated and cloned from soybean root mutant material M18, and constructs a plant overexpression vector of the gene And RNAi expression vector, through the transformation of soybean plants, the transformed plants have the function of resisting Phytophthora root rot, and provide genetic resources and basic materials for the cultivation of new varieties resistant to Phytophthora root rot.

The invention discloses a Coxsackie virus B5 type virus-like particle, its preparation method and application, and belongs to the technical fields of genetic engineering and biomedicine. The coxsackie virus type B5 virus-like particle is a recombinant baculovirus that expresses the P1 capsid protein gene and the 3CD protease gene of the coxsackie virus type B5 to infect Sf9 insect cells, and cultures to more than 90% of the Sf9 insect cells were obtained after infection with lesion. The invention also discloses the preparation method and application of the Coxsackievirus B5 type virus-like particle. The coxsackievirus type B5 virus-like particle of the present invention has a similar appearance structure and size to wild virus particles, and its immunogenicity reaches or even exceeds the level of wild inactivated virus, and can be used to prepare coxsackievirus type B5 Virus-like particle vaccines.

The invention is a method that producing neutral proteinase by a gene recombination Pichia.The character is producing neutral proteinase by Pichia pastoris. Using Pichia pastoris to express extraneous source protein has many advantages such as better expression, better stability, and stronger secretion. As Pichia pastoris can not synthesize Bacillus subtilis proteinase itself,this invention carried Bacillus subtilis proteinase gene into Pichia pastoris to help producing much more Bacillus subtilis proteinase.Because Pichia pastoris is easy to ferment in high density and it has highly secrection, it is easy to industrial produce cheap Bacillus subtilis proteinase.