31 results about "Proteinase Gene" patented technology

The present invention relates to a swine vesicular disease virus-like particle prepared by a gene recombination technique, as well as a preparation method thereof. More particularly, the invention relates to a method for preparing a swine vesicular disease virus-like particle, wherein the structural protein precursor (Pl) gene and 3CD protease gene of swine vesicular disease virus are inserted simultaneously into a baculovirus expression vector and are expressed in insect cells, so that the expressed 3CD protease enzyme digests the structural protein precursor into individual proteins which then form the capsid of swine vesicular disease virus by an self-assembly process.

The invention relates to the field of genetic engineering and fermentation engineering, and provides a candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste. The candida utilis double-gene co-expression strain is constructed by integrating carboxypeptidase and endoprotease genes onto a candida utilis genome through a candida utilis expression vector. According to the candida utilis double-gene co-expression strain capable of degrading the kitchen waste, and the candida utilis double-gene co-expression strain can specifically degrade the protein components in the kitchen waste and decompose and convert the protein components into small peptides and amino acids.

The invention provides a method for improved high secretory production of proteins. The present invention addresses the problem of providing a production system which achieves, in a host cell such as yeast, high secretory production of proteins, particularly proteins having a complex structure such as an S-S bond and which is so safe as to obviate explosion-proof equipment and is suitable for industrial production. The present invention provides a transformed yeast obtained by introducing a chaperone gene and disrupting aox1 and / or protease genes, and a method for producing proteins using the transformed yeast.

The invention provides an oryza sativa thioredoxin gene OsNDU, a protein, a vector, a host cell, a molecular marking method and an application of the molecular marking method. The OsNDU has a nucleotide sequence shown in SEQ ID No.1, a cDNA sequence of the OsNDU is shown in SEQ ID No.2, and a protein sequence of the OsNDU is shown in SEQ ID No.3. A scheme provided by the invention is a good example that thioredoxin participates in nilaparvata lugens resistance of oryza sativa, and the scheme has a certain reference value for understanding a function of thioredoxin and regulating and controlling resistance. A research of the OsNDU gene provides a good theoretical basis for a downstream molecular mechanism of nilaparvata lugens resistant genes of oryza sativa, and has reference significancefor researching a gene molecular function and breeding.

The invention belongs to the technical field of genetic engineering, and in particular relates to an aspergillus oryzae keratinase gene and its expression vector and application. The specific technical scheme is: a keratinase expression gene, the DNA sequence of which is shown in SEQ ID No:1. The gene expresses a keratinase, the amino acid sequence of which is shown in SEQ ID No:2. The invention provides a new gene resource capable of expressing keratinase, combines the gene with a plasmid, and transfers the gene into a bacterial strain to produce keratinase, thereby enriching the source of keratinase.

The invention provides rice thioredoxinase gene OsNDU, protein, carrier, host cell, molecular marker method and application. OsNDU has the nucleotide sequence shown in SEQ ID No.1, its cDNA sequence is shown in SEQ ID No.2, and its protein sequence is shown in SEQ ID No.3. The scheme of the present invention is a good example of the participation of thioredoxin enzyme in rice resistance to brown planthopper, which has certain reference value for understanding the function of thioredoxin enzyme and the regulation of resistance. The study of OsNDU gene provides a good theoretical basis for the downstream molecular mechanism of rice resistance to brown planthopper genes, and has reference significance for the study of gene molecular functions and breeding.

A cathepsin B gene of bollworm and its clone and recombinant expression process are disclosed. Its preparing steps include extracting overall RNA from bollworm, reverse transcription to synthesize cDNA, designing primer, polymerase chain reaction, multiplication purifying, cloning it to pGEM-T Easy vector, transferring DH5 alpha cells for sequence test, fast multiplication to obtain end-to-end CDNA (1310 bp), and expressing proteinase in prokaryotic and eukaryotic organisms to generate cathepsin B with hydrolysis activity.

The bean metalloprotease PvFtsH2 gene and its encoded protein and application relate to the field of genetic engineering, in particular to a plant metalloprotease PvFtsH2 gene and its encoded protein and application. The invention provides a bean FtsH metalloprotease PvFtsH2 gene, its encoded protein and application. The nucleotide sequence of the PvFtsH2 gene is shown in SEQ ID NO: 1 in the sequence listing. The amino acid sequence of the encoded protein is shown in SEQ ID NO:2. The present invention confirms that the PvFtsH2 gene plays a role in the light-inhibition response process through plant physiology and molecular biology methods, and the mutation of the gene leads to F under light-inhibition conditions. V / F M The value decreased, the chlorophyll content decreased, and the D1 protein accumulated. The invention has important application value in increasing the yield of kidney beans by improving the utilization rate of light energy.

The invention belongs to the technical field of molecular biology, and in particular relates to a method for highly expressing recombinant human fibroblast growth factor 21 (rhFGF21) gene by using bacillus subtilis. First, the present invention adjusts the transcription and translation efficiency of the target protein gene by adding different types of cistron cistron sequences at the 5' end of the target protein gene, thereby optimizing its soluble expression level, which can be applied to optimize the expression of different types of heterologous proteins . Secondly, the present invention also optimizes the disulfide bond-related genes to construct the rhFGF21 disulfide bond folding efficiency; overexpresses the molecular chaperone system to construct the protein folding efficiency suitable for rhFGF21; The stable Bacillus subtilis genetically engineered strain finally provides a kind of Bacillus subtilis genetically engineered strain capable of efficiently secreting and expressing rhFGF21 protein with good application prospects.

The invention proposes a multiple primer, a kit and a detection method for detecting fermentation conditions that affect the expression of beer yeast protease A gene, which belong to the field of food detection and can solve the traditional problem of detecting protease A that affects beer yeast protease A by measuring the activity of protease A. The fermentation conditions of gene expression amount have the problems of long time-consuming, cumbersome detection process and long detection cycle. The technical scheme includes the design of multiple primers, reverse transcription and synthetic cDNA template using the total RNA of yeast strains extracted under different fermentation conditions as a template, multiple PCR amplification reactions, capillary electrophoresis separation, and the use of GeXP system for capillary electrophoresis separation. The results were analyzed; using the internal reference gene as a control, the expression level of the protease A gene was calculated in combination with the peak area of ​​the corresponding peak of the key gene, and the fermentation conditions affecting the expression level of the protease A gene in S. cerevisiae were judged according to the expression level of the protease A gene.

The invention discloses a monoclonal cell strain stably expressing a serine protease gene, a preparation method of the monoclonal cell strain, a kit containing the monoclonal cell strain, and application of the monoclonal cell strain. The monoclonal cell strain stably expressing the serine protease gene is cultured in a culture plate, and cell culture liquid is removed; classical swine fever virusraw liquid is subjected to doubling dilution, and the diluted virus liquid is added into the culture plate with the cell culture liquid being removed; and culture continues, a classical swine fever virus TCID50 is judged through a CPE phenomenon, and the TCID50 is calculated through a Reed-Muench method. The advantages that the monoclonal cell strain is used for visual detection of the classicalswine fever virus, the classical swine fever virus TCID50 is detected through the detection kit, a fluorescent microscope and a classical swine fever detection antibody are not needed, result judgement of the classical swine fever virus TCID50 is simple, and experimental repeatability is good are achieved.

The invention discloses an artificially-synthesized LSL gene for encoding a laetiporus sulphureus mushroom lectin N-acetyllactosamine binding domain and an expression vector and host cell containing the LSL gene. The nucleotide sequence of the LSL gene is SEQ ID NO. 1. The invention further discloses a target protein expression with LSL used as the fusion tag and a purification method thereof. The method specifically relates to LSL gene synthesis, construction of the expression vector containing the LSL gene and the target protein gene, construction of the expression vector containing the LSL gene and a protease gene, expression and purification of fusion protein, LSL protein tag removal, target protein purification and the like. The method is simple and easy to implement, one-step purification of target protein is achieved, high-purity target protein can be obtained, the protein purification cost is lowered, the method can be widely applied to activity protein in the fields of biological medicine, veterinary medicine and the like and large-scale preparation of vaccine antigen in the industry of biological products, and high practical value is achieved.

The invention provides soybean subtilisin gene Gmsbt1.6-3, which is the soybean subtilisin gene Gmsbt1.6-3 isolated and cloned from soybean root mutant material M18, and constructs a plant overexpression vector of the gene And RNAi expression vector, through the transformation of soybean plants, the transformed plants have the function of resisting Phytophthora root rot, and provide genetic resources and basic materials for the cultivation of new varieties resistant to Phytophthora root rot.

The invention discloses a Coxsackie virus B5 type virus-like particle, its preparation method and application, and belongs to the technical fields of genetic engineering and biomedicine. The coxsackie virus type B5 virus-like particle is a recombinant baculovirus that expresses the P1 capsid protein gene and the 3CD protease gene of the coxsackie virus type B5 to infect Sf9 insect cells, and cultures to more than 90% of the Sf9 insect cells were obtained after infection with lesion. The invention also discloses the preparation method and application of the Coxsackievirus B5 type virus-like particle. The coxsackievirus type B5 virus-like particle of the present invention has a similar appearance structure and size to wild virus particles, and its immunogenicity reaches or even exceeds the level of wild inactivated virus, and can be used to prepare coxsackievirus type B5 Virus-like particle vaccines.

The invention is a method that producing neutral proteinase by a gene recombination Pichia.The character is producing neutral proteinase by Pichia pastoris. Using Pichia pastoris to express extraneous source protein has many advantages such as better expression, better stability, and stronger secretion. As Pichia pastoris can not synthesize Bacillus subtilis proteinase itself,this invention carried Bacillus subtilis proteinase gene into Pichia pastoris to help producing much more Bacillus subtilis proteinase.Because Pichia pastoris is easy to ferment in high density and it has highly secrection, it is easy to industrial produce cheap Bacillus subtilis proteinase.

The invention discloses a streptomyces trypsin gene, construction of a heterologous expression carrier and enzymatic properties of the enzyme, belonging to the field of biotechnology. The full length of the GM2938 gene described in the present invention is obtained by mining the full gene data of Streptomyces endogenous A249. The full length of the gene is 759 bp, encoding 253 amino acids, and the gene is expanded in vitro by PCR. The present invention realizes the heterologous cloning, expression and purification of trypsin GM2938 gene in Bacillus subtilis SCK6 for the first time, and carries out the characterization of trypsin properties. The trypsin has good enzymatic properties, which will have broad prospects in the application of leather technology and food industry.