A monoclonal cell line stably expressing serine protease, its preparation method, a kit containing the cell line and its application
A serine protease and stable expression technology, applied in the field of monoclonal cell lines and their preparation, and kits containing the cell lines, can solve the problems of poor repeatability, expensive detection, and the need for fluorescent microscopes, etc., and achieve simple and accurate results. Good experimental repeatability
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[0027] 1. Materials
[0028] ST cells were purchased from ATCC Cell Preservation Center, MEM Alph medium and 0.25% trypsin were purchased from Gibco Company, newborn bovine serum (NBS) was purchased from Wuhan Sanli Company, Puromycin was purchased from Suleibao Company, The 96-well cell culture plate was purchased from Corning Company, the classical swine fever E2 antibody was purchased from MEDLAN Company, the goat anti-mouse FITC secondary antibody was purchased from Invitrogen Company, and the plasmid PLJM1 RagB 99L and CSFV-DNS3 were donated by Ma Ningning Laboratory of Shenyang Pharmaceutical University. Primer synthesis and sequencing services were completed at Thermo. ;EasyPfu DNA polymerase, dNTP and nucleic acid electrophoresis Marker were purchased from Quanshijin Biotechnology Co., Ltd. Competent cells DH5á were purchased from Bomed Biotechnology Co., Ltd.; plasmid small extraction kit, plasmid large extraction kit, agarose gel Gel DNA recovery kit and erythrocyte...
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