Candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste and construction method of candida utilis double-gene co-expression strain

A technology for producing Candida utilis and kitchen waste, which is applied in the fields of genetic engineering and fermentation engineering, can solve problems such as the influence of hydrolysis effect and the inability of protease to achieve the effect of action, and achieves the effect of good efficiency and large amino acid yield.

Pending Publication Date: 2021-04-20
GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the complexity of protein components in food waste, a single protease often cannot achieve a good effect, and multiple enzymes are required to work together, and the ratio of different enzymes will also have a great impact on the hydrolysis effect

Method used

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  • Candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste and construction method of candida utilis double-gene co-expression strain
  • Candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste and construction method of candida utilis double-gene co-expression strain
  • Candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste and construction method of candida utilis double-gene co-expression strain

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Embodiment

[0044]The pMD19-X recombinant plasmid is obtained by recombining the amplified target gene with the pMD19-T vector, that is, the recombinant plasmid containing the target gene X, where X represents the pepA gene or pepF gene. Digest the recombinant plasmid containing pepA or pepF, and at the same time, digest the Candida utilis expression vectors pcGAPGA and pcTEF1GA respectively, and connect the expression vector pcGAPGA and the target gene pepA obtained after digestion to obtain the pcGAPGA-pepA recombinant plasmid , ligating the expression vector pcTEF1GA obtained after enzyme digestion and the target gene pepF to obtain the pcTEF1GA-pepF recombinant plasmid; pcGAPGA, the structure of pcTEF1GA and the construction process of the recombinant plasmid are as follows Figure 12 shown. After the pcTEF1GA-pepF recombinant plasmid was electrotransformed into Candida utilis, the recombinant Candida utilis containing the single gene of carboxypeptidase pepF was obtained, and the bio...

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Abstract

The invention relates to the field of genetic engineering and fermentation engineering, and provides a candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste. The candida utilis double-gene co-expression strain is constructed by integrating carboxypeptidase and endoprotease genes onto a candida utilis genome through a candida utilis expression vector. According to the candida utilis double-gene co-expression strain capable of degrading the kitchen waste, and the candida utilis double-gene co-expression strain can specifically degrade the protein components in the kitchen waste and decompose and convert the protein components into small peptides and amino acids.

Description

technical field [0001] The invention relates to the fields of genetic engineering and fermentation engineering, and more specifically relates to a dual-gene co-expression strain of Candida utilis that hydrolyzes protein components in kitchen waste and a construction method thereof. Background technique [0002] The protein component in kitchen waste is the main component in kitchen waste except carbohydrates and fat. After the food waste is fermented, the microorganisms basically exhaust the carbohydrates in the food waste, but there are more protein substances left. Therefore, if the protein components in food waste are directly discharged into the environment, it is very easy to cause environmental pollution. How to convert and utilize protein in food waste is a difficult problem. [0003] Microbial fermentation is being valued by the industry at home and abroad. Fermentation technology is becoming an effective way to comprehensively utilize food waste, and truly achieve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12N15/57C12N15/81C12R1/72
CPCC12P21/06C12N15/815C12N15/70C12N9/62C12Y304/23018C12Y304/24C12N9/50C12R2001/72C12N9/485C12N2800/102C12N2800/22
Inventor 刘泽寰林蒋海孙涛
Owner GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
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