Prokaryotic expression method for sea cucumber cathepsin

Abstract

The invention discloses a prokaryotic expression method for sea cucumber cathepsin, the prokaryotic expression method comprises the following steps of carrying out cloning on gene sequence of the sea cucumber cathepsin; constructing fusion expression vector with the gene sequence of the sea cucumber cathepsin on the basis of obtaining the sea cucumber cathepsin cloned gene sequence; transforming the fusion expression vector to expression host strain for inducing the expression of target protein. The prokaryotic expression method provided by the invention has the advantages that escherichia coli is adopted to express the sea cucumber cathepsin at low cost, and the quality of expression product is easy to control.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a prokaryotic expression method of sea cucumber cathepsin. Background technique [0002] Procollagen is a structural protein of body tissue, and its many physiological functions have been systematically studied and widely recognized. With the rapid development of the collagen industry, the demand for protease, the key production factor in the collagen enzymatic hydrolysis process, is also increasing. At present, the commonly used enzymes generally have the disadvantage of low enzymatic hydrolysis efficiency of collagen, and the use of genetic engineering technology to develop new collagenase can make up for this fatal defect, and the quality of collagenase products produced by bioengineering technology is easy to control and the production cost is low. low. Apostichopus japonicus has a very strong autolysis ability, the essence of which is the efficient enzymolysis of proteases in ...

AI Technical Summary

Problems solved by technology

However, the screening and functional research of sea cucumber cathepsin genes and the development of related protease products have not been carried out systematically.