Phaseolus vulgaris metalloproteinase PvFtsH2 gene as well as encoding protein and application thereof

A metalloprotease and gene coding technology, applied in the field of plant metalloprotease PvFtsH2 gene and its encoded protein, can solve the problems of photosynthetic function decline, etc., and achieve the effect of increasing the yield of kidney beans and improving the utilization rate of light energy

Active Publication Date: 2021-02-12
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Light is a necessary factor for chloroplast development and photosynthesis, but under high light intensity conditions, light energy exceeds the amount that the photosynthetic sy

Method used

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  • Phaseolus vulgaris metalloproteinase PvFtsH2 gene as well as encoding protein and application thereof
  • Phaseolus vulgaris metalloproteinase PvFtsH2 gene as well as encoding protein and application thereof
  • Phaseolus vulgaris metalloproteinase PvFtsH2 gene as well as encoding protein and application thereof

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Experimental program
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specific Embodiment approach 1

[0036] Embodiment 1: The nucleotide sequence of the phase bean metalloprotease PvFtsH2 gene in this embodiment is shown in SEQ ID NO: 1 in the sequence listing.

specific Embodiment approach 2

[0037] Specific embodiment 2: The amino acid sequence of the protein encoded by the bean metalloprotease PvFtsH2 gene in this embodiment is shown in SEQ ID NO:2.

specific Embodiment approach 3

[0038] Specific embodiment three: the application of the bean metalloprotease PvFtsH2 gene in regulating the photoinhibition response process of leguminous plants in this embodiment.

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Abstract

The invention discloses a phaseolus vulgaris metalloproteinase PvFtsH2 gene as well as an encoded protein and application thereof, relates to the field of gene engineering, and particularly relates toa plant metalloproteinase PvFtsH2 gene as well as an encoded protein and the application thereof. The invention provides a kidney bean FtsH metalloproteinase PvFtsH2 gene as well as an encoding protein and application thereof. A nucleotide sequence of the PvFtsH2 gene is shown as SEQ ID NO: 1 in a sequence table. The amino acid sequence of the encoded protein is shown as SEQ ID NO: 2. Phytophysiology and molecular biology methods prove that the PvFtsH2 gene plays a role in the photoinhibition response process, and mutation of the gene causes reduction of the FV/FM value, reduction of the chlorophyll content and D1 protein accumulation under the photoinhibition condition. The method has important application value in the aspect of increasing the yield of kidney beans by improving the lightenergy utilization rate.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a plant metalloprotease PvFtsH2 gene and its encoded protein and application. Background technique [0002] Bean production is group production, and bean production also refers to group production. There are two main ways to increase the yield of kidney beans: one is to increase the absorption of the root system. A strong root system and a large absorption surface area are fundamental to maintaining the water and nutrients required by the stems, leaves, flowers, fruits and other organs on the ground; the other is to increase photosynthesis. 90% of the output of leguminous crops comes from photosynthesis, and the rate and efficiency of photosynthesis directly determine the yield of crops. Breeding with high light efficiency has long been a hot spot in the field of crop photosynthesis research and breeding. Improving the efficiency of light energy absorption, transmission, and c...

Claims

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Application Information

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IPC IPC(8): C12N15/57C12N9/50C12N15/82A01H5/00A01H6/54
CPCC12N9/63C12N15/8261C12N15/8251
Inventor 徐坤夏正俊朱金龙吴红艳翟红
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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