Prokaryotic expression method of sea cucumber cathepsin

Abstract

The invention discloses a prokaryotic expression method of sea cucumber cathepsin. The method includes: cloning a gene sequence of the sea cucumber cathepsin; on the basis of acquiring a gene sequence clone of the sea cucumber cathepsin, constructing a fusion expression vector of the gene sequence of the sea cucumber cathepsin, and transforming the fusion expression vector to an expressing host cell so as to perform induced expression of a target protein. Therefore, the method has the advantages that the sea cucumber cathepsin is expressed with Escherichia coli at low cost and quality of an expressed product is easy to control.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a prokaryotic expression method of sea cucumber cathepsin. Background technique [0002] Procollagen is a structural protein of body tissue, and its many physiological functions have been systematically studied and widely recognized. With the rapid development of the collagen industry, the demand for protease, the key production factor in the collagen enzymatic hydrolysis process, is also increasing. At present, the commonly used enzymes generally have the disadvantage of low enzymatic hydrolysis efficiency of collagen, and the use of genetic engineering technology to develop new collagenase can make up for this fatal defect, and the quality of collagenase products produced by bioengineering technology is easy to control and the production cost is low. low. Apostichopus japonicus has a very strong autolysis ability, the essence of which is the efficient enzymolysis of proteases in ...

AI Technical Summary

Problems solved by technology

However, the screening and functional research of sea cucumber cathepsin genes and the development of related protease products have not been carried out systematically.