Prokaryotic expression method of sea cucumber cathepsin
A cathepsin and prokaryotic expression technology, which is applied in the field of prokaryotic expression of sea cucumber cathepsin, can solve the problems of sea cucumber cathepsin gene screening and functional research that have not yet been systematically carried out, and achieve the effect of easy control of the quality of expression products
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment
[0024] Embodiment: a kind of prokaryotic expression method of sea cucumber cathepsin, comprising, wherein: comprise to sea cucumber cathepsin gene sequence cloning; On the basis of obtaining sea cucumber cathepsin gene sequence clone, construct the fusion expression vector with sea cucumber cathepsin gene sequence, then The fusion expression vector is transformed into an expression host bacterium to induce the expression of the target protein; the sea cucumber cathepsin gene sequence contains the sequence of SEQ1. The expression host bacteria is Escherichia coli. The primers used to amplify the DNA fragments of the target protein used in the cloning of the sea cucumber cathepsin gene sequence are those that increase EcoRI and NotI recognition sites
[0025] Primer 1: F: 5'-CCGGAATTCATGCCAGACACTGTTGATT-3'; (SEQ3)
[0026] Primer 2: R: 5'-ATAGTTTAGCGGCCGCTTAGACAGTTGGGTAACTG-3'. (SEQ4)
[0027] The restriction endonucleases used to construct the fusion expression vector were E...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com