HIV stain drug-resistant phenotype analytical cell model and special pseudotype lentivirus therefor

Abstract

The invention discloses a cell model of HIV strain phenotypic drug resistance analysis and special pseudotype slow virus thereof; the invention constructs plasmid for expressing Gag protein of HIV, recombinant slow-virus plasmid for expressing reporter gene and helper plasmid for expressing Rev protein of HIV; under the effect of the helper plasmid pVSV-G, the three plasmid and HIV reverse transcriptase and the gene fragment of protease which are amplified from strain can obtain the pseudotype slow virus of the HIV reverse transcriptase and protease gene containing strain. The pseudotype slow virus is used for infecting the cells of mammals, thus obtaining the strain phenotypic resistance cell model based on the reporter gene. The cell model of the invention can carry out phenotypic resistance analysis to the strain, the reporter gene leads the cell model to have super high sensitivity, and the cell model of HIV strain phenotypic drug resistance analysis is applicable to the phenotypic drug resistance analysis of HIV strain in Chinese population.

Description

technical field [0001] The invention relates to a cell model for analyzing drug-resistant phenotypes of HIV strains and a special pseudotyped lentivirus thereof. Background technique [0002] Human immunodeficiency virus (human immunodeficiency virus, HIV), also known as HIV, the disease caused by HIV infection is called AIDS, and its full name is acquired immunodeficiency syndrome (acquired immunodeficiency syndrome, AIDS). Some or all of them are lost, the number of CD4+ cells decreases, and then opportunistic infections, tumors, etc. occur, and the clinical manifestations are various. The disease spreads rapidly, has a high fatality rate, and is currently incurable, which has attracted the attention of governments and societies in various countries. The prevalence of HIV is distributed worldwide. Africa is the birthplace and hardest hit area of ​​HIV, and Europe and America are also the main epidemic areas. In recent years, the prevalence of HIV in Asia has shown a trend...

AI Technical Summary

Problems solved by technology

[0006] The large number of applications of anti-HIV drugs has led to the emergence of drug resistance, so establishing a fast, efficient, and low-cost HIV strain resistance analysis technology is a key problem that needs to be solved urgently

At present, there are two main methods for analyzing the drug resistance of HIV strains: one is to determine the nucleotide sequence of the virus strain and compare it with the HIV drug resistance mutation database to predict whether the strain will develop drug resistance. That is, the genotype drug resistance analysis method has certain guiding significance, but it is not accurate enough; the other is the phenotypic drug resistance analysis method, that is, the resistance of the virus strain to a certain drug is detected through the drug resistance test. The result of this method is accurate. However, the operation is complicated, the cycle is long, and the virus often needs to be isolated, and the isolation and culture of HIV is not an easy task, and the success rate is not very high

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