Method for detecting Aeromonas hydrophila by FQ-PCR (fluorescent quantitative polymerase chain reaction)

Abstract

The invention provides a kit for detecting Aeromonas hydrophila by FQ-PCR (fluorescent quantitative polymerase chain reaction). In a reagent packed in the kit, a sense primer and a reverse primer and TaqMan probe have one or two groups of A group and B group. Compared with the prior art, as the based factors only exist in the virulent Aeromonas hydrophila strain, the method can accurately detect whether the virulent Aeromonas hydrophila strain exists in the sample, thereby improving the early warning accuracy of the disease. Simultaneously, if the detection result shows that the template contains the hemolysin gene (hlyA) or serine proteinase gene (ahpA) by using the A group or B group reagent, this indicates that the disease has certain pathogenicity; and if the detection result shows that the template contains the hemolysin gene (hlyA) and serine proteinase gene (ahpA) by using the two groups of reagents, this indicates that the disease has high pathogenicity.

Description

technical field [0001] The invention relates to a method for detecting Aeromonas hydrophila by using FQ-PCR, belonging to a determination or inspection method involving enzymes or microorganisms in chemistry. Background technique [0002] Aeromonas hydrophila (Aeromonas hydrophila) is widely distributed in nature, commonly found in fresh water, sewage, silt, soil and human feces, can cause various animal infections, and humans will be infected with Aeromonas hydrophila Symptoms such as food poisoning and diarrhea occur, and it is an opportunistic pathogen for immunosuppressed patients and patients with liver function diseases. In addition to causing huge economic losses to the aquaculture industry, Aeromonas hydrophila also has a very serious impact on public health, especially in terms of clinical diseases and food safety. [0003] The traditional detection methods of Aeromonas hydrophila are physiological and biochemical identification and immunological diagnosis, but bec...

AI Technical Summary

Problems solved by technology

[0003] The traditional detection methods of Aeromonas hydrophila are physiological and biochemical identification and immunological diagnosis, but because many biochemical characteristics of the bacteria are unstable and the serotypes are also complicated, it is easy to cause misjudgment

Therefore, there have been many reports on the PCR detection method of Aeromonas hydrophila, such as the Chinese invention patent application "Aquatic animal and human pathogenic bacteria-Aeromonas hydrophila gene diagnostic kit and detection method (public number CN1560275) ", China's invention patent application "PCR detection kit and detection method for Aquatic experimental animal swordtail fish pathogen - Aeromonas hydrophila (public number CN1978665)", China invention patent application "PCR detection reagent for Aeromonas hydrophila Kit and its detection method (publication number CN1506468)", Chinese invention patent application "a double PCR rapid detection kit and detection method for Aeromonas and Aeromonas hydrophila (publication number CN 101736073A)" and Chinese invention patent Application for "Reagents for detection of pathogenic Aeromonas hydrophila and its detection method (public number CN101418335)", etc., but all the above-mentioned PCR detection methods for Aeromonas hydrophila are ordinary PCR detection, and all of them are Qualitative rather than quantitative

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