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Fish serine proteinases and their pharmaceutical and cosmetic use

A technology of serine protease and fish serine, applied in cosmetics, cosmetic preparations, applications, etc.

Inactive Publication Date: 2002-07-03
布亚纳森·乔·布拉吉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, trypsin and chymotrypsin from cod are not multifunctional enzymes

Method used

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  • Fish serine proteinases and their pharmaceutical and cosmetic use
  • Fish serine proteinases and their pharmaceutical and cosmetic use
  • Fish serine proteinases and their pharmaceutical and cosmetic use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 prepares cod protease mixture

[0050] Thaw about 100 kg of cod offal and pour it into an extraction vessel filled with four times its volume of drinking cold water, adjusting the pH to 8-9 with sodium hydroxide solution. The mixture was stirred at 0-5°C for 2-6 hours. After a brief period of coarse settling (approximately 30 min), the aqueous extract was pumped from the remaining insoluble guts and filled into a settling vessel. The water extract was placed in a cooled precipitation container for precipitation for 24-60 hours. The supernatant was then pumped from the supernatant container to a holding container. These supernatants were ultrafiltered and concentrated 10-20 fold and diafiltered to an acceptable level of ionic strength (conductivity below 3 mS / cm). About 10-15 liters of ultrafiltered and diafiltered protein concentrate are obtained.

Embodiment 2

[0051] Example 2 Purification of cod trypsin from concentrated cod viscera extract

[0052] Take 10 liters of the ultrafiltered and diafiltered concentrate obtained in Example 1, and load it on a series of continuously connected chromatographic columns of about 1 liter. The first column was equipped with CM fast flow cation exchange resin (CM fast flow cation exchange resin) (Sweden, Pharmacia), the second column was equipped with DEAE fast flow anion exchange resin (DEAE fast flow anion exchange resin) (Sweden, Pharmacia), the third Each column was loaded with p-aminobenzamidine affinity ligand (Pharmacia, Sweden) coupled to Sepharose resin. These fractal columns had been pre-equilibrated with about 10 column volumes of 25 mM Tris buffer (pH 7.8, containing 2.5 mM calcium chloride) (buffer A). The concentrate was pumped onto the lamina column at a flow rate of 100 ml per minute. After the concentrated solution was applied to the column, 8 liters of buffer A was used to wash...

Embodiment 3

[0055] Example 3 Another large-scale purification method for extracting cod trypsin from concentrated cod viscera extract

[0056] Ten liters of the ultrafiltered and diafiltered concentrate obtained in Example 1 were added to the p-aminobenzamidine affinity ligand coupled to Sepharose resin (Pharmacia, Sweden). The column had been pre-equilibrated with about 10 column volumes of 25 mM Tris buffer (pH 7.8, containing 2.5 mM calcium chloride) (buffer A). The concentrate was pumped onto the chromatography fold at a flow rate of 100 ml per minute. When the concentrated solution was loaded onto the column, 8 liters of buffer A was used to wash the residual material from the column.

[0057] Then the affinity column was washed with 5 column volumes of high salt solution-25Mm Tris buffer (pH7.5, containing 0.5M sodium chloride and 2.5mM calcium chloride). Cod trypsin was desorbed from the affinity ligand and eluted from the column with an acidic solution, namely 2.5 mM acetic acid...

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Abstract

Fish derived serine proteinases including trypsins and chymotrypsin derived from cod such as Atlantic cod is used for treating and / or preventing a variety of diseases and disorders such as inflammatory diseases, infectious diseases caused by viruses, bacteria and fungal species and diseases where a receptor binding mechanism is involved in the pathogenesis. Pharmaceutical and cosmetic compositions comprising the proteinases are described.

Description

technical field [0001] The invention relates to the field of disease prevention and treatment and the wide application in the field of cosmetics, in particular to the use of the serine protease obtained from fish in pharmacy and cosmetics. In particular, pharmaceutically or cosmetically active compositions are provided as active ingredients comprising trypsin and / or chymotrypsin isolated from Atlantic cod, and serine proteases related to these enzymes. Background technique [0002] US Patents 4,801,451 and 4,963,491 disclose a mixture of exogenous and endogenous endopeptidases isolated from Antarctic krill (Euphasia superba) and the use of the mixture as a cleaning solution. US Patent 4,801,451 discloses the use of this enzyme to remove foreign matter and dead tissue from wounds. International Patent Application WO 85 / 04809 discloses the use of krill enzymes as digestion enhancers. EP-A1-0170115 discloses the use of krill enzymes to dissolve blood clots. However, all of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/76A01N37/18A61KA61K8/02A61K8/66A61K8/72A61K9/00A61K9/06A61K38/00A61K38/46A61K38/48A61K47/10A61P9/00A61P17/00A61P17/02A61P17/06A61P19/02A61P25/04A61P29/00A61P31/00A61P31/04A61P31/10A61P31/12A61P33/00A61P33/10A61P37/00A61P37/02A61Q19/00A61Q19/08C12N9/64
CPCA61Q19/00A61K8/66A61K38/4826A61K2800/28A61Q19/08A61K9/0014A61K9/06A61P17/00A61P17/02A61P17/06A61P19/02A61P25/04A61P29/00A61P31/00A61P31/04A61P31/10A61P31/12A61P33/00A61P33/10A61P37/00A61P37/02A61P9/00A61K38/46
Inventor 布亚纳森·乔·布拉吉
Owner 布亚纳森·乔·布拉吉
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