Microbial fluorescent staining solution and application thereof

Abstract

The invention relates to a microbial fluorescent staining solution and an application thereof. The microbial fluorescent staining solution comprises lectin, fluorescein, nucleic acid dye, a buffer solution, an anti-quenching agent, a bacteriostatic agent and water; the microbial fluorescent staining solution can be used in microbial dyeing, specific recognition of agglutinin on glycoprotein and specific staining of nucleic acid by nucleic acid dye are utilized, according to the invention, the fungi are blue, green or red, the bacteria are red, the morphology is combined to achieve the detection on the microorganisms in a vaginal secretion sample, a cervical exfoliated cell sample, a skin sample and a sputum sample, the detection result can be visualized, and the microorganism variety can be directly and reasonably judged.

Description

technical field

[0001] The invention belongs to the field of microbial labeling and dyeing, and in particular relates to a microbial fluorescent dyeing solution and an application thereof. Background technique

[0002] Microorganisms exist widely in nature and play an extremely important role in the protection of the human body. There are different types and quantities of microorganisms on the human body surface and the cavities connected to the outside world, such as the oral cavity, respiratory tract, intestinal tract, genitourinary tract, and skin tissue. Together with the human environment, they constitute a micro-ecosystem and maintain a dynamic balance. Once the balance is broken, it may cause related diseases. Therefore, the detection of microorganisms is of great significance for human protection and disease prevention, diagnosis and treatment.

[0003] The traditional microbial detection technology includes the culture identification method: inoculate the sample c...

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