Microbial fluorescent staining solution and application thereof

A fluorescent dyeing and microbial technology, which is applied in the field of microbial fluorescent dyeing solution, can solve the problems of personnel operation hazards, strong fluorescence interference of fibers under the microscope, interference of clinical workers' diagnosis, etc., and achieve the effect of reasonable judgment

Active Publication Date: 2020-04-17
江苏美克医学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this staining solution can only be used for staining fungi, and the Evans blue reagent with carcinogenic components is added, which is harmful to personnel operations
Moreover, the detection process is susceptible to environmental pollution, and the fiber under the microscope will have strong fluorescence interference, which is disturbing to the diagnosis of clinicians.

Method used

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  • Microbial fluorescent staining solution and application thereof
  • Microbial fluorescent staining solution and application thereof
  • Microbial fluorescent staining solution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The present embodiment provides a kind of preparation method of microbial fluorescent staining solution, specifically:

[0036] Add 500ml of purified water to the beaker, and add wheat germ factor, fluorescein isothiocyanate, propidium iodide, sodium dihydrogen phosphate, disodium hydrogen phosphate, glycerol and Proclin 300 according to the formula concentration in Table 1, and finally use Dilute the purified water to 1L to obtain the microbial fluorescent staining solution.

[0037] Microbial fluorescent staining liquid formula composition in the embodiment 1 of table 1

[0038]

[0039]

Embodiment 2、 Embodiment 3

[0041]Embodiments 2 and 3 provide a method for preparing a microbial fluorescent staining solution. The difference from Embodiment 1 is that the concentration of raw materials is different, and the specific formula is shown in Table 2.

[0042] Microbial fluorescence staining solution formula composition in table 2 embodiment 2,3

[0043] Composition Example 2 Concentration Example 3 Concentration Wheat Germ 5g / L 2.5g / L fluorescein isothiocyanate 1g / L 0.5g / L propidium iodide 50mg / L 25mg / L Sodium dihydrogen phosphate 50g / L 25g / L Disodium phosphate 200g / L 100g / L Glycerol 50ml / L 25ml / L Proclin 300 10g / L 5g / L purified water Set volume to 1L Set volume to 1L

Embodiment 4～ Embodiment 8

[0045] Embodiments 4 to 8 provide a method for preparing a microbial fluorescent staining solution. The difference from Example 3 is that the composition of the raw materials is different, but the concentrations of the same type of components are the same. The specific formula is shown in Table 3.

[0046] Microbial fluorescent staining liquid formula composition in the embodiment 4~8 of table 3

[0047]

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Abstract

The invention relates to a microbial fluorescent staining solution and an application thereof. The microbial fluorescent staining solution comprises lectin, fluorescein, nucleic acid dye, a buffer solution, an anti-quenching agent, a bacteriostatic agent and water; the microbial fluorescent staining solution can be used in microbial dyeing, specific recognition of agglutinin on glycoprotein and specific staining of nucleic acid by nucleic acid dye are utilized, according to the invention, the fungi are blue, green or red, the bacteria are red, the morphology is combined to achieve the detection on the microorganisms in a vaginal secretion sample, a cervical exfoliated cell sample, a skin sample and a sputum sample, the detection result can be visualized, and the microorganism variety can be directly and reasonably judged.

Description

technical field [0001] The invention belongs to the field of microbial labeling and dyeing, and in particular relates to a microbial fluorescent dyeing solution and an application thereof. Background technique [0002] Microorganisms exist widely in nature and play an extremely important role in the protection of the human body. There are different types and quantities of microorganisms on the human body surface and the cavities connected to the outside world, such as the oral cavity, respiratory tract, intestinal tract, genitourinary tract, and skin tissue. Together with the human environment, they constitute a micro-ecosystem and maintain a dynamic balance. Once the balance is broken, it may cause related diseases. Therefore, the detection of microorganisms is of great significance for human protection and disease prevention, diagnosis and treatment. [0003] The traditional microbial detection technology includes the culture identification method: inoculate the sample c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N21/64
CPCC12Q1/04G01N21/6486
Inventor 孟凡茹吴加一周淑鑫吴自学孙康俊黄宝福
Owner 江苏美克医学技术有限公司
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