An electrochemical biochip sensor array for rapidly detecting mycobacterium tuberculosis and a preparing method

Abstract

The invention belongs to the technical fields of bioelectrochemistry and biochip sensors and relates to an electrochemical biochip sensor array for mycobacterium tuberculosis 16SrDNA and for detecting mycobacterium tuberculosis and a preparing method thereof. Starting with molecular level detection of the mycobacterium tuberculosis 16SrDNA, Fe3O4@SiO2 composite nanometer particles and a nanometer Au structure material are adopted as mediators, a novel and functionalized nanometer electrochemical biochip superparamagnetism nano-detection microsphere sensor interface is designed, the mycobacterium tuberculosis is subjected to double enrichment extraction and purification detection, and the electrochemical biochip sensor array for rapidly detecting the mycobacterium tuberculosis is constructed. A clinical sample containing the mycobacterium tuberculosis is directly added into a magnetic reaction micropore. A mycobacterium tuberculosis 16SrDNA specific sequence can be subjected to double enrichment and biological stabilization through utilizing a capture probe labeled by the Fe3O4@SiO2 composite nanometer particles and a nanometer Au labeled detecting probe respectively. A high mycobacterium tuberculosis target molecule separating efficiency and high detection sensitivity are expected to be achieved by utilization of nano-detection microsphere superparamagnetism. In addition, target molecule "on-chip" separation is expected to be achieved through characteristics of the electrochemical biochip sensor array without the need of extra separating steps, thus largely simplifying operation procedures and shortening the detection time.

Description

technical field [0001] The invention belongs to the technical field of bioelectrochemistry and biochip sensing, and relates to a Mycobacterium tuberculosis 16S r An electrochemical biochip sensing array of DNA (ribosomal DNA) and a preparation method thereof are used for detecting Mycobacterium tuberculosis. Background technique [0002] Mycobacterium tuberculosis is the pathogen that causes pulmonary tuberculosis, which causes about 2 million deaths every year. It is also an important factor that induces respiratory tract infections and brings great harm to human health. The number of global tuberculosis patients is increasing rapidly at a rate of 2% per year. The incidence of tuberculosis in my country ranks second in the world, with an infection rate of 50%, and China is one of the 22 countries with a high burden of tuberculosis. One of the most important reasons for the high incidence of tuberculosis in countries with low per capita income is the lack of accurate, reliab...

AI Technical Summary

Problems solved by technology

Now there are many kinds of TB detection methods: (1) such as Mycobacterium tuberculosis culture method, although it has high specificity and accuracy, although the isolation culture method has high accuracy and sensitivity, it takes at least a few weeks (2) Mycobacterium tuberculosis rapid acid-fast staining method has low sensitivity, and the detection rate fluctuates greatly among people with different infection backgrounds

(3) Immunochromatographic detection technology is characterized by simple operation and fast detection speed; however, the detection markers are single, and the sensitivity and specificity are low