PCR (Polymerase Chain Reaction) high-resolution melting detection kit for 15 animal-derived components and detection method
An animal-derived component, high-resolution melting technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of cross-contamination of open-tube operation, complex array chip process, and complicated design of primers. The effect of overcoming competition problems, improving detection efficiency, and reducing reaction costs
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[0061] 1) Select the target gene and sequence alignment
[0062] Based on the mitochondrial genes of 15 species of cattle, buffalo, yak, goat, sheep, rabbit, horse, donkey, pig, dog, cat, mouse, fox, mink, and raccoon dog, 5 pairs of universal primers were selected, in order to theoretically analyze the 15 species For sequence differences, refer to the complete mitochondrial sequences of 15 mammals provided by NCBI, and compare the sequences of the amplified products of three pairs of primers (results in figure 1 ), it can be seen that there are differences in varying degrees between the amplified sequences of the three pairs of primers.
[0063] 2) Feasibility analysis
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