PCR (Polymerase Chain Reaction) high-resolution melting detection kit for 15 animal-derived components and detection method

Abstract

The invention discloses a PCR (Polymerase Chain Reaction) high-resolution melting detection kit for 15 animal-derived components and a detection method. According to the technical scheme, the invention discloses a PCR (Polymerase Chain Reaction) high-resolution melting detection kit for 15 animal-derived components and a detection method. Through primer optimization screening, three pairs of universal primers are adopted for combined detection, the melting temperature Tm of species is used as a characteristic value to establish a standard library, and 15 animal-derived components of cattle, buffalo, yak, goat, sheep, rabbit, horse, donkey, pig, dog, cat, mouse, fox, mink and raccoon dog in meat and meat products can be rapidly, simply and conveniently detected. The method is simple, convenient and rapid, agarose electrophoresis is not needed, cross contamination is avoided, and corresponding specific primers do not need to be designed for each species; by referring to PCA and LDA analysis methods in statistics, the detection result is more visual; compared with various traditional PCR technologies, the method has the advantages that the problem of competition among primers is solved, the detection efficiency is greatly improved, and the reaction cost is reduced.

Description

technical field [0001] The invention belongs to the field of safety detection of meat and meat products, and in particular relates to a PCR high-resolution melting detection kit and detection method for 15 kinds of animal-derived components. Background technique [0002] With the rapid development of social economy, food adulteration crimes driven by profiteering are increasing day by day. In recent years, due to the "horse meat incident" in Europe, Wal-Mart's "donkey meat adulteration" and other food fraud problems have emerged one after another, which has aroused people's great attention to the safety of meat products. [0003] At present, the adulteration of meat products in the Chinese market is mainly divided into three categories according to the form of adulteration. The first category is that merchants mix some low-priced meat (pig, chicken, duck) with high-priced meat (beef and mutton), and after some processing The processing methods make it difficult to distingui...

AI Technical Summary

Problems solved by technology

However, most of these methods are based on specific primers, and the design of primers is cumbersome and complicated; the process required for array chip detection is complicated, and the operation of opening tubes is easy to cause cross-contamination, which limits its popularization and application

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