High-salt heterotrophic nitrification-aerobic denitrification dephosphorization brachybacterium and application of brachybacterium in wastewater treatment

An aerobic denitrification and Brevibacterium microbacteria technology, which is applied in aerobic process treatment, biological water/sewage treatment, water/sludge/sewage treatment, etc. Effect

Active Publication Date: 2014-05-21
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the reports on high-salt wastewater at home and abroad focus on the removal of organic matter and nitrogen. At present, there are almost no reports on strains capable of removing phosphorus under high-salinity conditions, and the simultaneous removal of wastewater under high-salinity conditions. Nitrogen and phosphorus removal is a challenging subject

Method used

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  • High-salt heterotrophic nitrification-aerobic denitrification dephosphorization brachybacterium and application of brachybacterium in wastewater treatment
  • High-salt heterotrophic nitrification-aerobic denitrification dephosphorization brachybacterium and application of brachybacterium in wastewater treatment
  • High-salt heterotrophic nitrification-aerobic denitrification dephosphorization brachybacterium and application of brachybacterium in wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Implementation Example 1: Simultaneous Denitrification and Phosphorus Ability Determination of Brachybacterium at a Salinity of 3%

[0039] Inoculate the Brachybacterium strain (the same below) preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee (CGMCC for short) with the preservation number CGMCC No. Invade and maintain the growth vitality of the bacteria, and carry out enrichment culture. The cultured bacterial solution was centrifuged, washed three times with sterile water, and the optical density (OD 600 ) for the bacterial suspension of 1-2.

[0040] Take 10mL of the above bacterial suspension and add two test media containing 90mL of 3% salinity (calculated as NaCl) (0.94g glucose, 0.153g NH 4 Cl, 0.035g KH 2 PO 4 , 0.1gMgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, pH 7.0~7.5) Erlenmeyer flasks, sealed with 9 layers of gauze, and cultured in a shaker at 30°C and 150rpm. The culture medium that was not...

Embodiment 2

[0042] Implementation Example 2: Denitrification and phosphorus removal effects of strains under different salinity conditions

[0043] Using glucose as the carbon source and ammonia nitrogen as the nitrogen source, the Brachybacterium strains described in the examples were tested for their ability to remove ammonia nitrogen at different salinities. The specific implementation steps are as follows:

[0044] Inoculate the Brachybacterium strain into 100ml inorganic salt medium (0.94g glucose, 0.153g NH 4 Cl, 0.035g KH 2 PO 4 , 0.1gMgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, 30g-150g NaCl, pH7.0~7.5), pre-cultivate in a shaker at 30°C and 150rpm. When the strain grows to the late logarithmic growth phase, take 10ml of the bacterial solution and insert it into 90ml of fresh inorganic salts with a salinity (calculated as NaCl) of 1%, 3%, 5%, 8%, 10% and 13% for culture Culture medium was shaken at 30°C and 150 rpm in a shaker. The culture medium that was not inoculated with th...

Embodiment 3

[0047] Implementation example 3: Denitrification and phosphorus removal experiments of bacterial strains under different pH conditions

[0048] The Brachybacterium strain was inoculated in 100 ml of a 3% inorganic salt medium (as in Example 2) with a salinity (calculated as NaCl), and pre-cultured in a shaker at 30° C. and 150 rpm. When the strain grows to the late logarithmic phase, take 10ml of the bacterial liquid and put it into 90ml of fresh inorganic salt medium, adjust the pH of the medium to 6.5-7.0, 7.0-7.5 and 7.5-8.0 respectively, at 30°C Shaking culture was carried out in a shaker at 150 rpm. The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the same conditions. Take a small amount of reaction solution at 6h, 12h, 18h, 24h, 36h and 48h, a part of which is directly used to measure the optical density of the bacteria, and the rest is centrifuged at 8000rpm for 10min, and the supernatant is take...

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Abstract

The invention relates to high-salt heterotrophic nitrification-aerobic denitrification dephosphorization brachybacterium and an application of the brachybacterium in wastewater treatment. The brachybacterium has high endurance capacity to a high-salt environment, grows well under a high-salt condition, can metabolize by utilizing organic carbon as a sole carbon source and ammonia nitrogen as a sole nitrogen source, directly converts ammonia nitrogen into a gas product through the heterotrophic nitrification-aerobic denitrification action, and achieves denitrification; and the brachybacterium can also take nitrate nitrogen as a sole nitrogen source, converts nitrate nitrogen into a gas product through the aerobic denitrification action, and can take inorganic phosphorus in under an aerobic condition to convert into a self-component so as to remove phosphorus from sewage. The brachybacterium is applicable to high-salt wastewater treatment, can achieve synchronous removal of nitrogen and phosphorus under a single aerobic condition, is conducive to effectively achieving biological decarbonization, dephosphorization and denitrification under the high-salt condition, and has a wide application prospect.

Description

technical field [0001] The invention relates to a strain of Brachybacterium and its application in high-salt wastewater treatment. The strain has the function of heterotrophic nitrification-aerobic denitrification, and can realize the process of simultaneous nitrification and denitrification denitrification under high-salt conditions, and at the same time complete the removal of phosphorus-containing pollutants in sewage. Background technique [0002] High-salt wastewater refers to wastewater with a total salt content (calculated by NaCl content) of at least 1%, which contains a large amount of inorganic salts, such as Cl, in addition to a large amount of high-concentration organic matter. - 、Na + , Ca 2+ , SO 4 2- Wait. With the direct use of seawater and the massive discharge of high-salt industrial wastewater, new requirements have been put forward for the treatment of high-salt wastewater. The high-concentration organic matter or nutrients in high-salt wastewater, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/01
CPCY02W10/10
Inventor 倪晋仁邓若男
Owner PEKING UNIV
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