Adapter modified nano silver, reagent kit and application thereof

An aptamer and nano-silver technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as increasing the workload of operators, polluting the environment, and interfering with normal results.

Active Publication Date: 2012-07-04
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, RAST also has many disadvantages: high cost, long time-consuming, radioactive isotope is easy to expire and pollutes the environment, different reference sera of kits from different sources are not easy to compare with each other, and normal results may be interfered when the serum to be ...

Method used

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  • Adapter modified nano silver, reagent kit and application thereof
  • Adapter modified nano silver, reagent kit and application thereof
  • Adapter modified nano silver, reagent kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Embodiment 1: Preparation of nano-silver solution.

[0079] Under ice bath conditions, add 40mL 2mmol / L silver nitrate solution dropwise to 80mL 3mmol / L sodium borohydride solution, stir continuously until the reaction is complete, and continue stirring to room temperature to obtain nano silver solution.

Embodiment 2

[0080] Embodiment 2: Preparation of nano-silver solution.

[0081] Under ice bath conditions, add 40mL of 10mmol / L silver nitrate solution dropwise to 40mL of 30mmol / L sodium borohydride solution, stir continuously until the reaction is complete, and continue stirring to room temperature to obtain the nano silver solution.

Embodiment 3

[0082] Embodiment 3: the preparation of nano-silver solution.

[0083] Add 40mL of 20mmol / L silver nitrate solution dropwise to 400mL of 10mmol / L sodium borohydride solution in an ice bath, keep stirring until the reaction is complete, and continue stirring to room temperature to obtain the nano silver solution.

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Abstract

The invention discloses adapter modified nano silver. A nano silver ball with the diameter of 10nm to 30nm is used as a kernel; and the outer surface of the silver ball is bonded with an adapter chain and an oligonucleotide chain. The invention also discloses a preparation method of the adapter modified nano silver, a reagent kit comprising the adapter modified nano silver, and application of the adapter modified nano silver to preparation of a reagent for detecting IgE (Immunoglobulin E). When used for carrying out the detection of IgE, the nano silver material provided by the invention has high sensitivity, short detection time (about 2h to 3h), simple and feasible operation and visual detection result; downhole semi-quantitative detection can be achieved in a visual mode without the need of an especially expensive detection apparatus; and the adapter modified nano silver has low cost, can realize bulk detection, and is suitable for popularization and utilization.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to an aptamer-modified nano-silver for detecting IgE, a kit and application thereof. Background technique [0002] Allergic reactions are a large class of diseases common in modern society, which are caused by eating or contacting allergens in various environments. Most of these reactions are type I hypersensitivity reactions caused by IgE immunoglobulin E . The detection of IgE in serum samples can help in medical diagnosis. [0003] The IgE content in normal human serum is extremely low, about 50 ng / mL. Commonly used methods for detecting IgE include radioimmunosorbent assay and enzyme-linked immunosorbent assay. [0004] Radioimmunosorbent test (RAST) is a method for detecting serum IgE by absorbing anti-IgE antibody onto a solid-phase carrier. The double-antibody sandwich method is commonly used clinically, and filter paper is mostly used as the carrie...

Claims

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Application Information

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IPC IPC(8): G01N33/531
Inventor 许丹科李周敏赵熙睿李慧魏霞
Owner NANJING UNIV
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