Adapter modified nano silver, reagent kit and application thereof

Abstract

The invention discloses adapter modified nano silver. A nano silver ball with the diameter of 10nm to 30nm is used as a kernel; and the outer surface of the silver ball is bonded with an adapter chain and an oligonucleotide chain. The invention also discloses a preparation method of the adapter modified nano silver, a reagent kit comprising the adapter modified nano silver, and application of the adapter modified nano silver to preparation of a reagent for detecting IgE (Immunoglobulin E). When used for carrying out the detection of IgE, the nano silver material provided by the invention has high sensitivity, short detection time (about 2h to 3h), simple and feasible operation and visual detection result; downhole semi-quantitative detection can be achieved in a visual mode without the need of an especially expensive detection apparatus; and the adapter modified nano silver has low cost, can realize bulk detection, and is suitable for popularization and utilization.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to an aptamer-modified nano-silver for detecting IgE, a kit and application thereof. Background technique [0002] Allergic reactions are a large class of diseases common in modern society, which are caused by eating or contacting allergens in various environments. Most of these reactions are type I hypersensitivity reactions caused by IgE immunoglobulin E . The detection of IgE in serum samples can help in medical diagnosis. [0003] The IgE content in normal human serum is extremely low, about 50 ng / mL. Commonly used methods for detecting IgE include radioimmunosorbent assay and enzyme-linked immunosorbent assay. [0004] Radioimmunosorbent test (RAST) is a method for detecting serum IgE by absorbing anti-IgE antibody onto a solid-phase carrier. The double-antibody sandwich method is commonly used clinically, and filter paper is mostly used as the carrie...

AI Technical Summary

Problems solved by technology

However, RAST also has many disadvantages: high cost, long time-consuming, radioactive isotope is easy to expire and pollutes the environment, different reference sera of kits from different sources are not easy to compare with each other, and normal results may be interfered when the serum to be tested contains the same specific IgG

However, the reaction time of each well is very important to the repeatability of the results. If more than one microplate is used, it is required to repeat the measurement of the standard curve separately, which will undoubtedly increase the workload of the operator.

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