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Two phase Roe's culture medium and preparation method thereof

A technology similar to Roche culture medium and Roche culture medium, applied in the field of medical testing, can solve the problems of long time, low positive rate, inability to meet clinical diagnosis and treatment and disease prevention, etc., to promote rapid growth, improve culture positive rate, Conducive to the effect of rapid preliminary identification

Inactive Publication Date: 2005-11-23
胡忠义
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  • Description
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AI Technical Summary

Problems solved by technology

This culture medium takes a long time (usually 4-8 weeks), and the positive rate is not high (usually 10%-30%), which is far from meeting the needs of clinical diagnosis and treatment and disease prevention.

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0033] Roche's culture medium among the present invention is exactly the improved Roche's culture medium general at home and abroad at present, adds (also can not add) 4-6ml 0.1% chromogen TTC solution in 1000ml improved Roche's culture medium; , growth promoters and antibacterial agents.

[0034] The composition of the basic medium is as follows: copper sulfate 0.001 g, 0.0015 g or 0.002 g, zinc sulfate 0.001 g, 0.0015 g or 0.002 g, calcium chloride 0.001 g, 0.0016 g or 0.002 g, magnesium sulfate 0.01 g, 0.018 g or 0.02 g gram, 0.01 gram, 0.016 gram or 0.02 gram of oleic acid, 0.05 gram, 0.07 gram or 0.1 gram of ferric ammonium citrate, 0.1 gram, 0.15 gram or 0.2 gram of sodium citrate, 0.2 gram, 0.3 gram or 0.4 gram of ammonium sulfate , Sodium pyruvate 0.5g, 0.7g or 1g, Tween-800.5g, 0.7g or 1g, Asparagine 1g, 1.3g or 2g, Potassium dihydrogen phosphate 1g, 1.5g or 2g, 2 grams, 3 grams or 4 grams of sodium dihydrogen phosphate, 8 milliliters, 10 milliliters or 12 milliliter...

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Abstract

The present invention discloses a preparation method of a new type incubation medium, belonging to the medical inspection field. The method can promote growth of mycobacteria. The incubation medium comprises a solid part and a liquid part: the solid part is the modified Roche's incubation medium that is generally used at home and abroad and the liquid part consists of basic incubation medium, growth promoter and bacteria inhibitor. The incubation medium can not only promote growth of mycobacteria, but also inhibit other bacteria. The growing mycobacteria can form violet red bacteria colony on the solid inclined plane of incubation medium, which is beneficial to elementary appraisal. The incubation medium can shorten incubation time obviously, increase incubation positive rate considerably, very suitable for fast inspection of mycobacteria for the hospitals and the sanitation and anti-epidemic institutions.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a novel culture medium capable of promoting the rapid growth of mycobacteria and a preparation method thereof. Background of the invention [0002] Mycobacteria are a group of bacteria widely distributed in nature, some of which can cause human and animal disease. In recent years, the number of diseases caused by mycobacterial infection has been widely reported at home and abroad. In particular, secondary infections in immunocompromised patients and outbreaks of nosocomial infections have increased significantly. For the diagnosis of infectious diseases caused by mycobacteria, the diagnosis can be confirmed as long as the mycobacteria are cultured from the sample to be tested. [0003] At present, the modified Roche medium is commonly used at home and abroad to isolate and culture mycobacteria (see the Chinese Medical Association's clinical technical operati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 胡忠义
Owner 胡忠义
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