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Method for producing A ring degradation products through plant sterol fermentation

A technology for phytosterols and degradants, which is applied in the field of fermenting phytosterols to produce A-ring degradants, can solve the problems of difficulty in product accumulation, great environmental impact, and difficulty in extraction, and achieves the effects of improving yield and inhibiting degradation.

Inactive Publication Date: 2015-03-11
江西赣亮医药原料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] A ring degradant (δ-Lactone) is an important intermediate for the synthesis of estrone, estradiol and their derivatives, but during the conversion process, the steroid nucleus A ring and B ring are opened, which is very easy to degrade and the product is difficult to accumulate. Difficult to meet industrial standards
The Chinese patent application with application number 201410029845.3 discloses a Mycobacterium fortuitousus and its application in fermentative production of δ-lactone, but soybean oil is used in fermentative transformation, which is difficult to extract and has a great impact on the environment

Method used

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  • Method for producing A ring degradation products through plant sterol fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of Liquid Inoculum Containing Mycobacterium fortuitum

[0023] a1) Inclined seed culture

[0024] Slant seed medium is sterilized, inoculated with Mycobacterium fortuitum after cooling, and cultivated at 30°C for 3 to 5 days. The formula of described slant seed medium is as follows: yeast extract 12g / L, sodium nitrate 6.35g / L, potassium dihydrogen phosphate 1.0g / L, dipotassium hydrogen phosphate 2.0g / L, glucose 6g / L, potassium chloride 0.2 g / L, magnesium sulfate heptahydrate 0.8g / L, agar 20g / L, adjust the pH value to 7.1-7.2.

[0025] a2) Primary seed cultivation

[0026] The liquid seed medium was sterilized, cooled to room temperature, and the colony cultivated by the above-mentioned slope seeds was added to the primary liquid seed medium for cultivation. The cultivation temperature was 30° C., the cultivation rotation speed was 190 rpm, and the cultivation time was 48 hours. The formula of the primary liquid seed medium is as follows: corn steep liquor...

Embodiment 2

[0037] Preparation of Liquid Inoculum Containing Mycobacterium fortuitum

[0038] a1) Inclined seed culture

[0039] Slant seed medium was sterilized, inoculated with Mycobacterium fortuitum after cooling, and cultured at 32°C for 3 to 5 days. The formula of described slant seed medium is as follows: yeast extract 13g / L, sodium nitrate 6.4g / L, potassium dihydrogen phosphate 1.1g / L, dipotassium hydrogen phosphate 2.1g / L, glucose 6.5g / L, potassium chloride 0.15g / L, magnesium sulfate heptahydrate 0.9g / L, agar 22g / L, adjust the pH value to 7.1-7.2.

[0040] a2) Primary seed cultivation

[0041] The liquid seed medium was sterilized, cooled to room temperature, and the colony cultivated by the above-mentioned slope seeds was added to the primary liquid seed medium for cultivation. The cultivation temperature was 32° C., the cultivation speed was 200 rpm, and the cultivation time was 45 hours. The formula of the primary liquid seed medium is as follows: corn steep liquor 21g / L, s...

Embodiment 3

[0052] Preparation of Liquid Inoculum Containing Mycobacterium fortuitum

[0053] a1) Inclined seed culture

[0054] Slant seed medium was sterilized, inoculated with Mycobacterium fortuitum after cooling, and cultured at 28°C for 3 to 5 days. The formula of described slant seed medium is as follows: yeast extract 11g / L, sodium nitrate 6.3g / L, potassium dihydrogen phosphate 0.9g / L, dipotassium hydrogen phosphate 1.9g / L, glucose 5.5g / L, potassium chloride 0.1g / L, magnesium sulfate heptahydrate 0.7g / L, agar 18g / L, adjust the pH value to 7.1-7.2.

[0055] a2) Primary seed cultivation

[0056] The liquid seed medium was sterilized, cooled to room temperature, and the colony cultivated by the above-mentioned slope seeds was added to the primary liquid seed medium for cultivation. The cultivation temperature was 28° C., the cultivation rotation speed was 180 rpm, and the cultivation time was 50 hours. The formula of the primary liquid seed medium is as follows: corn steep liquor ...

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Abstract

The invention discloses a method for producing A ring degradation products through plant sterol fermentation. The method comprises the following steps: (A) preparing a liquid inoculant containing mycobacterium fortuitum; (B) carrying out fermentation culture; (C) extracting and separating so as to obtain the A ring degradation products. The provided fermentation method for preparing A ring degradation products is carried out in an oil-free condition, thus the ring opening of A ring and B ring is effectively inhibited, thus the degradation is inhibited, the yield is increased, and the weight yield can reach 35 to 40%.

Description

technical field [0001] The invention relates to a method for preparing an important intermediate of steroid hormones, in particular to a method for fermenting phytosterols to produce A ring degradation products. Background technique [0002] In modern times, the research results on the preparation of steroid intermediates from phytosterols using microbial methods have been widely reported and studied. The earliest related reports can be traced back to 1937. Mamoli and Vercellone published two patents Ber.70470 and Ber.702079 , discloses the reduction of 17-keto-steroids to 17-β-hydroxysteroids by yeast fermentation. Thereafter, Peterson and Murray disclosed in US Patent No. 2602769 a method of 11-a-hydroxylation of progesterone using Rhizopus fungus. In 1972, Kraychy et al disclosed in US Patent No. 3684657 the method of using Mycobacterium SP. NRRL B-3683 to degrade the 17-position aliphatic chain to prepare 4-AD, ADD. In 1973, Marsheck et al disclosed in U.S. Patent No. ...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12R1/33
Inventor 应正平蒋青锋陈小良杨坤
Owner 江西赣亮医药原料有限公司
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