Devices and methods for measurement of a
hydroxysteroid analyte concentration in a host is provided, including a sensor housing, the sensor housing comprising an implantable
analyte sensing portion configured to generate a
signal associated with a concentration of a
hydroxysteroid analyte, the analyte sensing portion comprising at least one (NAD(P)+)- dependent
hydroxysteroid dehydrogenase enzyme; and at least one NAD(P)H oxidase. Detecting a
signal corresponding to the hydroxysteroid analyte of the implantable portion after implantation of the implantable portion is also provided.