Mycobacterium cosmeticum with capacity of degrading benzene compound and application thereof

A technology of benzene series compounds and mycobacteria, which is applied in microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of rareness, the degradation efficiency needs to be further improved, and achieve the effect of rapid degradation

Active Publication Date: 2010-01-13
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These degrading strains can only degrade one or two kinds of benzene compounds, and the degradation efficiency of most s...

Method used

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  • Mycobacterium cosmeticum with capacity of degrading benzene compound and application thereof
  • Mycobacterium cosmeticum with capacity of degrading benzene compound and application thereof
  • Mycobacterium cosmeticum with capacity of degrading benzene compound and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation, purification and identification of Mycobacterium cosmeticum byf-4 1. Isolation and purification of Mycobacterium cosmeticum byf-4

[0030] Mycobacterium cosmeticum byf-4 is a gram-negative bacterium that was domesticated, isolated and purified from the activated sludge in the aeration tank of Zhejiang Zhenhai Refining and Chemical Wastewater Treatment Plant. Specific steps are as follows:

[0031] (1) Directed domestication of sludge

[0032] Activated sludge from the aeration tank of Zhejiang Zhenhai Refining and Chemical Wastewater Treatment Plant was taken. The activated sludge was subjected to directional domestication of benzene series compounds (BTEX), and every 5 days was a domestication cycle, and the domestication period was 3 months until the activated sludge achieved stable and efficient degradation of benzene series compounds.

[0033] (2) Screening and purification of bacterial strains

[0034] The benzene series degrading bacteria ...

Embodiment 2

[0044] Embodiment 2: Detection of Mycobacterium cosmeticum byf-4 to BTEX biodegradation performance

[0045] 1. Biodegradation characteristics of Mycobacterium cosmeticum byf-4 on BTEX at different temperatures

[0046] The degradation experiment of Mycobacterium cosmeticum byf-4 on BTEX was carried out at different temperatures, and it was found that it has a high ability to degrade benzene compounds at 25-35°C. From the perspective of practical application, 30°C is the most suitable temperature. At this time The removal rate is the highest, and the specific implementation steps are as follows:

[0047] In the inorganic medium containing benzene compounds as the only carbon source (the final concentration is composed of: benzene 50mg / L, toluene 50mg / L, ethylbenzene 50mg / L, o-xylene 50mg / L, NH 4 Cl 0.1g / L, MgCl 2 ·6H 2 O 0.08g / L, K 2 HPO 4 0.25g / L, CaCl 2 2H 2 O 0.1g / L, the solvent is water, pH 7.2~7.4) add fresh cultured byf-4 bacterial suspension, OD 600 0.2, the ino...

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Abstract

The invention provides a mycobacterium cosmeticum byf-4 with the capacity of degrading a benzene compound and an application thereof. The mycobacterium cosmeticum byf-4 is stored in Chinese typical culture storage center which has an address: Wuhan University Wuhan China, 430074, a storage date: Oct. 29, 2008 and a storage number: CCTCC No. M 208180. The invention has the advantages that the strain can rapidly degrade various benzene compounds with high efficiency and has an important meaning to the engineering practice of biologically purifying contaminants caused by the benzene compounds and the derivatives thereof.

Description

(1) Technical field [0001] The invention relates to a strain of mycobacterium (Mycobacterium cosmeticum) byf-4 capable of degrading benzene series compounds and its application. (2) Background technology [0002] Benzene compounds are a class of volatile monocyclic aromatic compounds, including benzene, toluene, ethylbenzene, xylene, etc., referred to as BTEX, widely used in petroleum, chemical, pesticide, textile, papermaking, paint, shoemaking and other industries It is a kind of toxic compound widely distributed in the environment. BTEX has "three effects", has been listed as a priority pollutant by many countries, and has been confirmed as a strong carcinogen. It is very important and urgent to develop BTEX pollution control technology in wastewater and waste gas. Currently, biodegradation technology is one of the most effective ways to degrade such substances. [0003] One of the keys to using biological methods to purify toxic compounds such as BTEX in wastewater an...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34C02F1/58B01D53/84C12R1/32
CPCY02A50/20
Inventor 陈建孟张丽丽王家德朱润晔
Owner ZHEJIANG UNIV OF TECH
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