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Origin fermentation method and origin fermentation product prepared by same

A kind of origin and fermenter technology, applied in the direction of fermentation, skin care preparations, pharmaceutical formulas, etc., can solve the problems of complex enzyme preparation process steps, consumption of many types of excipients, increased production costs, etc., to achieve reasonable composition, small molecular weight, step-by-step simple effect

Active Publication Date: 2014-11-19
北京翰若生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the enzyme preparation process has complex steps, high energy consumption, and many types of auxiliary materials; due to the use of liquid saccharification process, its production cost increases; a small amount of organic solvent remains in the product, which affects the quality; therefore, this process limits its use in Applications in Industry

Method used

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  • Origin fermentation method and origin fermentation product prepared by same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Get fresh aloe leaves, beating and crushing to obtain leaf slurry, and the crushed particle size is greater than 15 mesh;

[0056] Put the leaf slurry into the fermentation tank, add the aqueous solution of ammonium sulfate, ammonium dihydrogen phosphate, ammonium bicarbonate, and corn steep liquor dry powder into the fermentation tank after continuous sterilization. The composition of the fermentation tank is: by mass percentage, the leaves 40% of slurry, 1.9% of ammonium sulfate, 0.7% of ammonium dihydrogen phosphate, 1.1% of ammonium bicarbonate, 0.5% of corn steep liquor dry powder, the balance is water, and the substances contained in the fermentation tank account for 1 / 3 of the volume of the fermentation tank;

[0057] The early stage of fermentation is 2 hours, the middle stage is 4 hours, and the late stage is 2 hours; the temperature in the early stage is 35°C, the temperature in the middle stage is 37°C, and the temperature in the late stage is 38°C. The relati...

Embodiment 2

[0060] Get fresh ginkgo tender leaves, beating and crushing to obtain leaf slurry, and the crushed particle size is greater than 15 mesh;

[0061] Put the leaf slurry into a fermentation tank, add the aqueous solution of ammonium sulfate, ammonium dihydrogen phosphate, ammonium bicarbonate, and corn steep liquor dry powder into the fermentation tank after continuous sterilization, and the composition of the fermentation tank is: by mass percentage , 50% of leaf slurry, 2.0% of ammonium sulfate, 0.8% of ammonium dihydrogen phosphate, 1.2% of ammonium bicarbonate, 0.6% of corn steep liquor dry powder, the balance is water, and the substances contained in the fermentation tank account for 1 / 2 of the volume of the fermentation tank ;

[0062] The early stage of fermentation is 3 hours, the middle stage is 6 hours, and the late stage is 3 hours; the temperature in the early stage is 36°C, the temperature in the middle stage is 36°C, and the temperature in the late stage is 37°C. Th...

Embodiment 3

[0065] Take fresh young verbena leaves, beat and crush to obtain leaf slurry;

[0066] Put the leaf slurry into a fermenter, add sterilized ammonium sulfate, ammonium dihydrogen phosphate, ammonium bicarbonate, corn steep liquor dry powder aqueous solution into the fermenter, the composition of the fermenter is as follows: In terms of mass percentage, 60% of leaf slurry, 2.1% of ammonium sulfate, 0.8% of ammonium dihydrogen phosphate, 1.3% of ammonium bicarbonate, 0.7% of corn steep liquor dry powder, and the balance is water. 2 / 3;

[0067] The early stage of fermentation is 2.5 hours, the middle stage is 5 hours, and the late stage is 2.5 hours; the temperature in the early stage is 35.5°C, the temperature in the middle stage is 36°C, and the temperature in the late stage is 37°C. The relative dissolved oxygen in the early stage is 70%, and the relative dissolved oxygen in the middle stage The amount is 70%, and the relative dissolved oxygen amount in the later stage is 0%, ...

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Abstract

The invention relates to the field of biological fermentation and in particular relates to an origin fermentation method and an origin fermentation product prepared by the same. The origin fermentation method comprises the following steps: acquiring fresh leaves, pulping and crushing to obtain a leaf slurry; placing the leaf slurry into a fermentation tank, adding sterilized ammonium sulphate, ammonium dihydrogen phosphate, ammonium hydrogen carbonate and an aqueous solution of corn steep liquor dry powder into a fermentation tank so as to be fermented; carrying out cell breakage, ultrafiltration, low-temperature drying and concentrating on a fermentation product, and packaging, so that the origin fermentation product is obtained. The origin fermentation method provided by the invention has the advantages that fresh leaves are crushed, bacteria and enzymes carried by the leaves are directly utilized for carrying out fermentation, no organic solvent, saccharifying enzyme or yeast is added in a fermentation process, the fermentation time is short, main ingredients of the obtained origin fermentation product are active substances beneficial to skin, such as micromolecule plant proteins, plant growth factors, plant activity auxin and part of cell growth factors, and the quality of the obtained origin fermentation product is high.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method of origin fermentation and the obtained origin fermentation product. Background technique [0002] Enzymes were first discovered from fermented yeast, which are called enzymes in European and American countries, and in Japan and other countries. Enzymes and enzymes are generally distinguished in this way, the whole of the enzyme is called an enzyme, and a member of the whole of the enzyme is called an enzyme. Enzymes exist in living organisms, use protein as a carrier, and have catalytic vitality. [0003] In the world we live in, enzymes are ubiquitous organisms that are tenacious but sensitive to the environment in which they live. The ability of enzymes is very great. Human beings can hardly survive without enzymes. Enzymes can serve chemists to manufacture and synthesize many very special substances, such as special medicines, food and so on. From the functi...

Claims

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Application Information

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IPC IPC(8): C12P21/00A61K8/64A61Q19/02
Inventor 黄海军王若林
Owner 北京翰若生物科技有限公司
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