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Method for producing glutamic acid through double-feeding fermentation optimization of corn steep liquor and glucose

A fed-batch fermentation and glucose technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of increasing energy consumption of fermentation production, affecting bacterial growth, prolonging fermentation time, etc., and shortening the adaptation period and bacteria doubling time, production cost reduction, and simple process operation

Active Publication Date: 2013-08-14
山东祥维斯生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, glutamic acid fermentation mainly adopts the method of adding enough corn steep liquor and glucose to the fermentation medium, adding glucose during or later in the fermentation process, and maintaining the sugar content in the fermentation broth within a certain range for fermentation. There is an influence of substrate concentration on the osmotic pressure of the bacteria, which affects the growth of the bacteria to a certain extent, prolongs the fermentation time, and increases the energy consumption of fermentation production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The strain used is a glutamic acid temperature-sensitive mutant strain (Brevibacterium glutamicum); culture method: wash the slant bacteria with 10ml sterile water, transfer to a 5000mL seed bottle containing 1200mL seed medium, The culture temperature is 32°C, 160rpm, shaking culture for 16h to the logarithmic phase, and all the seed liquid is inserted into the 15L fully automatic control system containing the fermentation medium (initial corn steep liquor and glucose addition amount is 5g / L) according to the inoculum amount of 12%. In the fermenter, the initial culture temperature is controlled at 32°C, and the pH value is adjusted to 7.0 by adding liquid ammonia dropwise; the dissolved oxygen is controlled in a staged oxygen supply mode: 20% for 0~10h, 5% for 10~out of the tank; at the beginning of fermentation, Add corn steep liquor at an amount of 15g / L, and control the whole process for 7 hours; add concentrated glucose solution until the end of fermentation, and c...

Embodiment 2

[0030]The strain used is a glutamic acid temperature-sensitive mutant strain (Corynebacterium glutamicum); culture method: Wash the slant bacteria with 10ml sterile water, transfer to a 5000mL seed bottle containing 1200mL seed medium, and cultivate Temperature 32°C, 160rpm, shaking culture for 16h to the logarithmic phase, all the seed liquid was added to a 15L fully automatic controlled fermentation containing fermentation medium (initial corn steep liquor and glucose addition 10g / L) according to the inoculation amount of 12% In the tank, the initial culture temperature is controlled at 31°C, and the pH value is adjusted to 7.2 by adding liquid ammonia dropwise; the dissolved oxygen is controlled in a staged oxygen supply mode: 20% from 0 to 12 hours, and 5% from 12 to the tank; when the fermentation starts, corn Add 12g / L of slurry, and control the whole process for 8 hours; add 80% glucose concentrate until the end of fermentation, and control the amount of residual sugar i...

Embodiment 3

[0035] The strain used is a glutamic acid temperature-sensitive mutant strain (Brevibacterium glutamicum); culture method: wash the slant bacteria with 10ml sterile water, transfer to a 5000mL seed bottle containing 1200mL seed medium, Cultivate at 32°C, 160rpm, vibrate for 15 hours to the logarithmic phase, and insert the seed liquid into the fermentation medium (glucose 5g / L, corn steep liquor 5g / L, K 2 HPO 4 6g / L, MgSO 4 ·7H 2 O 2g / L, VB1 150μg / L, VH 700μg / L, FeSO 4 ·7H 2 O, MnSO 4 ·H 2 O each 25mg / L, betaine 2g / L, urea 0.6g / L. In a 15L fully automatic control fermenter (sterilized at 121°C for 15min), the initial culture temperature was controlled at 32°C, and the pH value was adjusted to 7.1 by adding liquid ammonia dropwise; dissolved oxygen was controlled by staged oxygen supply mode: 20% for 0~9h, 9 Hours to put in the tank is 5%; at the beginning of fermentation, corn steep liquor is added in an amount of 16g / L, and the whole process is controlled for 6 hours;...

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Abstract

The invention discloses a method for producing glutamic acid through double-feeding fermentation optimization of corn steep liquor and glucose, which comprises the following steps of: in fermentation culture of glutamic acid, initially adding 1-10g / L of glucose to a fermentation culture medium, and initially adding 1-10g / L of corn steep liquor; after the fermentation culture starts, starting feeding the corn steep liquor and glucose at the same time, wherein 5-19g / L of corn steep liquor is fed and completely added within 5-10 hours since the fermentation starts, and the total amount of the corn steep liquor is 15-20g / L; feeding the concentrated solution of glucose until the fermentation is over; maintaining the amount of residual sugar in the fermentation culture medium at 0.05-10g / L; and controlling the pH value of the liquid ammonia in the whole process of fermentation to 6.8-7.3. The method disclosed by the invention effectively increases the growth speed of thalli and shortens the adaptation period in the initial stage of thallus fermentation and the thallus doubling time, thus shortening the overall fermentation time and reducing the overall production cost of glutamic acid.

Description

technical field [0001] The invention relates to the technical field of amino acid production by fermentation, in particular to a method for optimizing the production of glutamic acid by double-flow fermentation of carbon source and organic nitrogen source. Background technique [0002] At present, the situation of my country's glutamic acid production industry is grim: on the one hand, the market demand for glutamic acid continues to expand, and the output cannot meet the demand; Amino acid profits fell. Therefore, seeking a better process to optimize fermentation conditions, improve fermentation quality and yield, and save energy to reduce production costs is an important issue faced by all glutamic acid production enterprises. [0003] At present, glutamic acid fermentation mainly adopts the method of adding enough corn steep liquor and glucose to the fermentation medium, adding glucose during or later in the fermentation process, and maintaining the sugar content in the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12R1/13C12P13/14C12R1/15
Inventor 马兴群刘雨宋琦吕丽娟
Owner 山东祥维斯生物科技股份有限公司
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