Production method for functional amino-acid humic-acid microecological preparation for aquatic animal and poultry

A micro-ecological preparation and functional technology, which is applied in the production field of functional amino acid humic acid micro-ecological preparations for aquatic livestock and poultry, can solve problems such as lack of research, and achieve the effects of improving the breeding environment, enriching nutrition, and preventing and curing diseases. Good results

Inactive Publication Date: 2016-03-30
辽宁威兰生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many studies on the three major categories of Bacillus subtilis, Bacillus lichen

Method used

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  • Production method for functional amino-acid humic-acid microecological preparation for aquatic animal and poultry
  • Production method for functional amino-acid humic-acid microecological preparation for aquatic animal and poultry
  • Production method for functional amino-acid humic-acid microecological preparation for aquatic animal and poultry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] (1) Preparation of arginine concentrated bacterial liquid wet powder:

[0082] The arginine strain is inoculated into the arginine fermentation medium, and the arginine fermentation medium is: 1% of glucose, 3% of corn steep liquor, 1% of ammonium sulfate, and 2% of sodium glutamate. The inoculation amount is 5%.

[0083] Obtain arginine fermentation liquid through 64 hours of fermentation. Put 16 tons of arginine fermentation broth into a 20-ton container and let it stand. Add the flocculant sodium polyacrylate with a final concentration of 250ppm to the arginine fermentation broth, adjust the pH value to 6.5 with sulfuric acid with a mass fraction of 40%, react at a temperature of 50°C, and the reaction time is 80min, and then use a pressure of 3MPa The reacted arginine fermentation hydraulic pressure is poured into a filter press, and the filter press time is 60 minutes to obtain arginine concentrated bacterial liquid wet powder with a water content of 35%.

[008...

Embodiment 2

[0113] The functional amino acid humic acid micro-ecological preparation product for aquatic livestock and poultry obtained in Example 1 was applied in the young ginseng breeding in aquaculture, and the test group 1 used functional amino acid humic acid micro-ecological preparations for aquatic livestock and poultry with 5g of each water body per day. Ecological preparation, 5g amino acid microecological preparation (produced by Hebei Jingjing Pharmaceutical Biological Feed Co., Ltd.) for each water body of test group 2; 5 milliliters of seawater for each water body of the control group every day, and the test results are shown in Table 2: test 1 and matched group t test The results of mathematical statistical analysis of P value results are shown in Table 3: the results of mathematical statistical analysis of test 2 and control group t test P value results are shown in Table 4:

[0114] Table 2 Effect of functional amino acid humic acid microecological preparations for aquatic...

Embodiment 3

[0132] The functional amino acid humic acid microecological preparation product obtained in Example 1 for aquatic livestock and poultry was added into the feeding test in the whole ration of livestock and poultry breeding in beef cattle breeding, and the addition amount of test group 1 was 2% amino acid microecological Preparation product (produced by Hebei Jingjing Pharmaceutical Biological Feed Company); test 2 is the functional amino acid humic acid microecological preparation product for aquatic livestock and poultry with 2% addition, and the control group is 2% beef cattle complete ration. The retest results are shown in Table 5;

[0133] Table 5. Effects of functional amino acid humic acid microecological preparations for aquatic livestock and poultry on daily gain of beef cattle

[0134]

[0135] It can be seen from Table 5 that the daily gain of the test group was 3.60kg, the ratio of feed to meat was 1.39:1, the daily gain of the control group was 3.50kg, and the r...

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Abstract

The invention discloses a production method for a functional amino-acid humic-acid microecological preparation for aquatic animal and poultry. The production method comprises: employing arginine concentrated-bacterial-liquid wet powder, humic acid powder, corn-steep-liquor dry powder, soybean meal and wheat bran as fermentation raw materials, and employing bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae and lactobacillus acidophilus as beneficial microbial flora; separately performing single-colony separation, solid seed culture and liquid seed culture on the four microorganisms, mixing seed liquids of the four microorganisms, performing solid piling fermentation, and introducing air for aerobic solid fermentation, so as to obtain the microecological preparation. By employing the arginine concentrated-bacterial-liquid wet powder, humic acid powder and corn-steep-liquor dry powder as the main fermentation raw materials, the product is high in content of protein, amino acids, short-chain peptide and compound enzyme. The product is abundant in nutrition, good in disease-preventing disease-controlling effect, and obvious in growth promoting effect of cultured animals. The product also is capable of improving culture environment and purifying water quality, and is a green high-quality functional microecological preparation for aquatic animal and poultry.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a production method of a functional amino acid humic acid microecological preparation for aquatic livestock and poultry. Background technique [0002] Bacillus subtilis is an aerobic microorganism capable of producing spores. It belongs to prokaryotes in taxonomy, with single cells (0.3~0.5×1.5~3.0μm), arranged in pairs or chains, Gram-positive, without capsule, with perinatal flagella, and able to move. The spores are large (0.2~0.4×1.0~1.5μm), elliptical to columnar, located in the center of the fungus or slightly biased. After the formation of spores, the bacterial body does not expand, and the surface of the colony is rough, stained white or yellowish, and often forms wrinkles in liquid culture. Mainly distributed in oceans, rivers, lakes, activated sludge and soil. Bacillus subtilis has fast propagation speed and is easy to cultivate. It can secrete proteas...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12N1/20C12N1/18C12R1/10C12R1/125C12R1/23C12R1/865
Inventor 薛德林刘志国李铁军战晓燕习凤安王晓霞
Owner 辽宁威兰生物技术有限责任公司
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