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Fermentation method of erythromycin fermentation liquid

A fermentation method and erythromycin technology, applied in the field of medicine and biology, can solve the problems of low utilization rate of sugar and nitrogen, large consumption of raw materials, etc., and achieve the effect of improving the utilization rate of sugar and nitrogen, reducing the degree of pollution, and improving bioavailability

Active Publication Date: 2012-04-11
YILI CHUANNING BIOTECH CO
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, domestic existing research about erythromycin fermentation technology, such as the erythromycin fermentation technology optimization research that people such as Han Dequan of East China University of Science and Technology carry out (East China University of Science and Technology Engineering Master's Degree Thesis in 2004); Also described in the Chinese patent specification CN101134972B Improve the fermentation process of erythromycin by applying the technology of bacteria residue, etc. With the production of high-yield strains and continuous improvement of fermentation methods, the level of fermentation units has been continuously improved, but the problems of large consumption of raw materials and low utilization rate of sugar and nitrogen have not been solved.

Method used

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  • Fermentation method of erythromycin fermentation liquid

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Experimental program
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Effect test

Embodiment 2

[0056] (1) Erythromycin seed liquid culture: 1. female slant spore preparation: the erythromycin Streptomyces (producing bacteria) spores frozen in the sand tube were inoculated on the sterilized slant medium, and the culture medium consisted of: Starch 0.2g, corn steep liquor 0.1g, agar 0.2g, glucose 0.8g, sodium chloride 1.5g, calcium chloride 0.8g, dilute to 100ml with tap water; culture at 34°C for 8 days; Spore preparation: Inoculate the female slant spores on the sterilized slant medium, the medium consists of: 0.2g starch, 0.15g corn steep liquor, 0.6g agar, 0.2g glucose, 0.8g sodium chloride, calcium chloride 0.8g, dilute to 100ml with tap water; cultivate for 8 days at a temperature of 34°C; ③Seed culture: inoculate the spores of the sporozoites into the sterilized seed medium, and undergo three-stage seed expansion cultivation to obtain erythromycin prime seed liquid;

[0057] (2) Fermentation: Inoculate erythromycin seed liquid in 20% ratio in the sterilized medium...

Embodiment 3

[0061] (1) Erythromycin seed liquid culture: 1. female slant spore preparation: the erythromycin Streptomyces (producing bacteria) spores frozen in the sand tube were inoculated on the sterilized slant medium, and the culture medium consisted of: Starch 0.4g, corn steep liquor 0.08g, agar 0.8g, glucose 0.6g, sodium chloride 1.2g, calcium chloride 0.4g, dilute to 100ml with tap water; culture at 33℃ for 9 days; Spore preparation: Inoculate the female slant spores on the sterilized slant medium, the medium consists of: 0.3g starch, 0.1g corn steep liquor, 0.7g agar, 0.5g glucose, 1.0g sodium chloride, calcium chloride 1.0g, dilute to 100ml with tap water; cultivate for 9 days at a temperature of 33°C; ③Seed culture: Inoculate the spores of the sporozoites into the sterilized seed medium, and undergo three-stage seed expansion cultivation to obtain erythromycin prime seed liquid;

[0062] (2) Fermentation: inoculate erythromycin seed liquid in 15% ratio in the sterilized medium,...

Embodiment 4

[0066] (1) Erythromycin seed liquid culture: 1. female slant spore preparation: the erythromycin Streptomyces (producing bacteria) spores frozen in the sand tube were inoculated on the sterilized slant medium, and the culture medium consisted of: Starch 0.9g, corn steep liquor 0.07g, agar 0.9g, glucose 0.3g, sodium chloride 1.0g, calcium chloride 0.2g, dilute to 100ml with tap water; culture at 33℃ for 7 days; Spore preparation: Inoculate the female slant spores on the sterilized slant medium, the medium consists of: 0.3g starch, 0.1g corn steep liquor, 0.7g agar, 0.5g glucose, 1.0g sodium chloride, calcium chloride 1.0g, dilute to 100ml with tap water; cultivate for 9 days at a temperature of 33°C; ③Seed culture: Inoculate the spores of the sporozoites into the sterilized seed medium, and undergo three-stage seed expansion cultivation to obtain erythromycin prime seed liquid;

[0067] (2) Fermentation: inoculate erythromycin seed solution in 15% ratio in the sterilized mediu...

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Abstract

The invention discloses a fermentation method of erythromycin fermentation liquid, comprising the following steps of: inoculating streptomyces erythreus (producing bacterium) spores, culturing in a slant culture medium, performing three-stage seed intermediate culture, then inoculating into a sterilized culture medium, introducing sterile air and stirring, adding nutrient substances, and culturing. According to the invention, by optimizing the culture medium and improving the fermentation method, the utilization ratios of raw material such as glucose, soybean meal, corn steep liquor and the like are improved, the kinds and consumption of raw and auxiliary materials for fermentation are reduced, and the bioavailability of the raw and auxiliary materials is high.

Description

technical field [0001] The invention relates to a microbial fermentation method, in particular to a low-sugar and low-nitrogen fermentation method for erythromycin, and belongs to the field of medical biotechnology. Background technique [0002] Erythromycin is an alkaline antibiotic isolated from the culture medium of Streptomyces erythreus in 1952. Erythromycin has a broad-spectrum antibacterial effect, and has a strong inhibitory effect on Gram-positive bacteria, such as Staphylococcus, Streptococcus pyogenes, Streptococcus viridans, Streptococcus pneumoniae, Streptococcus faecalis, Clostridium, and Bacillus diphtheria . It also has considerable inhibitory effect on Gram-negative bacteria, such as Neisseria gonorrhoeae, Helicobacter, pertussis, Brucella, Legionella, Haemophilus influenzae, and Bacteroides. my country has successfully trial-produced it since 1957 and gradually put it into mass production. [0003] At present, domestic existing research about erythromyci...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12R1/465
Inventor 邹太平王利春刘思川梁隆程志鹏邓芸黄海玉
Owner YILI CHUANNING BIOTECH CO
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