Process for producing propionate

A propionate and process technology, which is applied in the production process field of microbial fermentation to produce propionate, can solve the problem of high total cost, and achieve the effects of saving production cost, low price and increasing yield

Inactive Publication Date: 2012-10-03
沈阳科纳提克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the domestic industry often uses yeast powder and peptone as the organic nitrogen source to ferment and produce propionic acid, and the obtained propionic acid is further rea...

Method used

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  • Process for producing propionate
  • Process for producing propionate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Fermentation process of the present invention produces calcium propionate

[0024] Strains: Propionibacterium acidipropionici (CGMCC1.2232);

[0025] Anaerobic bottle seed medium formula: yeast powder 10g / L, trypticase soybean broth 10g / L, K 2 HPO 4 2g / L, KH 2 PO 4 1g / L;

[0026] Seed pot medium formula (weight and volume percentage): 6% glycerin, 1% yeast extract, 0.5% tryptone, 0.3% dipotassium hydrogen phosphate, 0.3% potassium dihydrogen phosphate, and the balance is water;

[0027] Fermentation medium formula: corn steep liquor dry powder 10g / L, soybean powder 5g / L, K 2 HPO 4 0.5g / L, KH 2 PO 4 0.1g / L, glycerin 10g / L. Among them, corn steep liquor dry powder was purchased from Shandong Yuncheng Kangyuan Biotechnology Co., Ltd., soybean powder was purchased from Anyang Mantianxue Food Engineering Co., Ltd., and the trade name was deodorized sterilized soybean protein powder.

[0028] Inoculate the Propionibacterium acidogens preserved in conv...

Embodiment 2

[0032] Example 2 Existing fermentation process produces calcium propionate

[0033] Use the fermentation medium of existing technology (formulation is: yeast powder 5g / L, peptone 5g / L, K 2 HPO 4 0.5g / L, KH 2 PO 4 0.1g / L, glycerol 10g / L), calcium propionate was produced under the same conditions as those used in Example 1. The content of propionic acid is measured by high performance liquid chromatography. When the content of propionic acid is 31.1g / L, the pH value does not change any more, the content of propionic acid no longer increases, and the fermentation stops. The time used for fermentation is 239h, and the final volume of fermented liquid is 7.44m 3 .

[0034] Filter the obtained fermented liquid through a filter press, take the filtrate, send it to a spray tower for spray drying, and obtain 309.6 kg of calcium propionate after drying.

[0035] Obtainable by the fermentation effect of embodiment 1, embodiment 2, technique of the present invention has received bet...

Embodiment 3

[0036] Embodiment 3 Fermentation process of the present invention produces calcium propionate

[0037] Strains: Propionibacterium acidipropionici (CGMCC1.2232);

[0038] Anaerobic bottle seed medium formula: yeast powder 10g / L, trypticase soybean broth 10g / L, K 2 HPO 4 2g / L, KH 2 PO 4 1g / L;

[0039]Seed pot medium formula (weight and volume percentage): 6% glycerin, 1% yeast extract, 0.5% tryptone, 0.3% dipotassium hydrogen phosphate, 0.3% potassium dihydrogen phosphate, and the balance is water;

[0040] Fermentation medium formula: corn steep liquor dry powder 25g / L, soybean powder 15g / L, K 2 HPO 4 3.5g / L, KH 2 PO 4 2.5g / L, glycerol 50g / L. Among them, corn steep liquor dry powder was purchased from Shandong Yuncheng Kangyuan Biotechnology Co., Ltd., soybean powder was purchased from Anyang Mantianxue Food Engineering Co., Ltd., and the trade name was deodorized sterilized soybean protein powder.

[0041] Inoculate the Propionibacterium acidogens preserved in con...

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Abstract

The invention discloses a microbial fermentation culture medium and a process for producing propionate by using the fermentation culture medium. According to the fermentation process, the fermentation culture medium in which corn steep liquor dry powder and soybean meal are taken as an organ nitrogen source is used, so that the fermentation time is shortened, and the yield of the propionate is also improved; and raw materials are industrial by-products, so that the cost is saved, and the environment problem is also solved.

Description

technical field [0001] The invention relates to a microbial fermentation medium and a production process for producing propionate by microbial fermentation. Background technique [0002] Propionates such as calcium propionate, sodium propionate, potassium propionate, etc. can effectively inhibit mold and aerobic bacillus, and are basically harmless to the human body. They are good antiseptic preservatives. Therefore, it is widely used, especially in medicine, food, feed, spices, organic synthesis and so on. [0003] Propionate has great application value, and its production methods mainly include chemical synthesis and microbial fermentation. Because of the cost problem, the current industry is mainly based on chemical synthesis. In recent years, with the increasing attention to food safety issues and the increasing awareness of global environmental protection, more and more people have carried out a lot of research work on the production of propionate by microbial ferment...

Claims

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Application Information

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IPC IPC(8): C12P7/52C12R1/01
Inventor 姜黎黎栾娈李平顺
Owner 沈阳科纳提克生物科技有限公司
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