Method for preparing feedstuff yeast from maize peel hydrolysis solution

A technology for feed yeast and hydrolyzate, applied in animal feed, animal feed, fungi, etc., can solve the problems of low utilization rate of corn soaking water and corn husk, achieve significant economic and social benefits, reduce costs, and produce continuous production. Effect

Inactive Publication Date: 2007-10-17
SHANDONG LUZHOU FOOD GROUP
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  • Summary
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of low utilization rate of corn husks and corn husks, the present invention provides a method for preparing feed yeast by fermenting corn husk hydrolyzate as a raw material. The method can complete fermentation in a non-sterile environment, and the produced feed yeast It can ensure the nutritional integrity of yeast and can completely replace fish meal for livestock and poultry breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for preparing feed yeast with corn husk hydrolyzate as raw material, the steps are as follows, all in mass ratio:

[0036] (1) Culture medium preparation

[0037] According to the growth characteristics of Candida utilis, the growth of Candida utilis mainly depends on growth factors such as nitrogen source, carbon source, inorganic salts, trace elements in the medium, and these nutrients are mainly provided by the above-mentioned ingredient medium; The base is prepared as follows:

[0038] ① The concentration of corn soaking water with 4% protein content and 1% reducing sugar content is 10%; adjust the pH to 6.0 with 3mol / L NaOH solution, and filter for later use;

[0039] ②Corn bran is hydrolyzed with sulfuric acid, the acid concentration is 1%; acid solution: corn bran (dry basis) = 10:1; hydrolysis temperature is 105°C, hydrolysis time is 5 hours; after hydrolysis is completed, filter, and the filtrate is separated by simulated moving bed chromatography Th...

Embodiment 2

[0053] A method for preparing feed yeast with corn husk hydrolyzate as raw material, the steps are as follows, all in mass ratio:

[0054] (1) Culture medium preparation

[0055] ① The concentration of corn soaking water with 5.5% protein content and 1.5% reducing sugar content is 12%; use 5mol / L NaOH solution to adjust the pH to 6.0, and filter for later use;

[0056] ②Corn bran is hydrolyzed with sulfuric acid, the acid concentration is 3%; acid solution: corn bran (dry basis)=8:1; hydrolysis temperature is 110°C, hydrolysis time is 4 hours; after hydrolysis is completed, filter, and the filtrate is separated and recovered by simulated moving bed chromatography Sulfuric acid, the recovered hydrolyzate is stored for subsequent use;

[0057]③ Mix the above-mentioned corn soaking water and the hydrolyzed solution after acid recovery at a mass ratio of 1.5:1, adjust the pH to 5.0 with hydrochloric acid, sterilize at 80°C for 25 minutes, and use it as a feed yeast culture medium...

Embodiment 3

[0070] A method for preparing feed yeast with corn husk hydrolyzate as raw material, the steps are as follows, all in mass ratio:

[0071] (1) Culture medium preparation

[0072] ① The concentration of corn soaking water with 7% protein content and 2.0% reducing sugar content is 15%; use 8mol / L NaOH solution to adjust pH=6.0, filter for later use;

[0073] ②Corn bran is hydrolyzed with sulfuric acid, the acid concentration is 8%; acid solution: corn bran (dry basis)=5:1; hydrolysis temperature is 120°C, hydrolysis time is 3.5 hours; after hydrolysis is completed, filter, and the filtrate is separated by simulated moving bed chromatography Sulfuric acid is recovered, and the recovered hydrolyzate is stored for subsequent use;

[0074] ③ Mix the above-mentioned corn soaking water and the hydrolyzed solution after acid recovery in a mass ratio of 1:1, adjust the pH to 6.0 with phosphoric acid, sterilize at 75°C for 30 minutes, and use it as a feed yeast culture medium for subseq...

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PUM

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Abstract

The present invention relates to method for preparing feedstuff yeast using corn husk hydrolyzed liquor as material. Acid is separated and recovered from the hydrolyzed liquor adopting simulated moving bed chromatogram separation system. The normal neutralization is substituted. The method is : mixing corn-soaking water, corn husk hydrolyzed liquor for preparing culture medium, carrying out three-stage culture at bevel medium, and ferment culture, concentration and evaporation, spray drying for preparing feedstuff yeast. The inventive medium is enriched with nitrogen source, carbon, inorganic salt, microelement and vitamin, growth factor etc. The inventive method can ensure the feedstuff yeast nutrient integrity, and substitute for the fish powder in fowl and stock breeding and effectively reduce the cost. The present invention promote the additive value of product, reduce the environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for preparing feed yeast, in particular to a method for preparing feed yeast by using corn husk hydrolyzate as a raw material. Background technique [0002] Corn soaking water and corn hulls are by-products of the starch sugar industry; corn soaking water is rich in amino acids, minerals and various growth factors, and starch sugar companies generally concentrate it into corn steep liquor, which is used as a nitrogen source for microbial pharmaceuticals and fermentation Industry; corn hulls are mainly used in the livestock industry as fiber feed. However, with the continuous expansion of the starch sugar industry in recent years, the supply of corn steep liquor and corn bran has exceeded the demand, and the sales of corn steep liquor are not smooth, which seriously hinders the development of the starch sugar industry; and many manufacturers wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K1/16A23K10/12
Inventor 牛纪超赵玉斌王新增李法田张杰刘培政牛继星
Owner SHANDONG LUZHOU FOOD GROUP
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