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Surface-modified fluorescent quantum dot/silica composite microspheres, preparation method thereof, and application thereof

A technology of fluorescent quantum dots and composite microspheres, which is applied to surface-modified fluorescent quantum dots/silica composite microspheres. Its preparation and application fields can solve the problems that cannot be directly used as fluorescent probes, and achieve stable optical properties. Highly biologically active, easy-to-operate effects

Inactive Publication Date: 2012-10-31
INST OF CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Recently, silica fluorescent nanoparticles compounded with quantum dots with controllable core-shell structure have achieved a series of research results (Adv. Mater. 2005, 17, 2354; Chem. Mater. 2007, 19, 4123; Chem. Mater.2010, 22, 420; 200410009941), however, these composite microspheres with high fluorescence efficiency cannot be directly used as fluorescent probes, because anti-nonspecific adsorption and active reactive groups on the surface are necessary for the preparation of molecular probes based on nanoparticles condition

Method used

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  • Surface-modified fluorescent quantum dot/silica composite microspheres, preparation method thereof, and application thereof
  • Surface-modified fluorescent quantum dot/silica composite microspheres, preparation method thereof, and application thereof
  • Surface-modified fluorescent quantum dot/silica composite microspheres, preparation method thereof, and application thereof

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preparation example Construction

[0039] The preparation method of anti-bioabsorption containing fluorescent quantum dots / silica fluorescent microspheres of the present invention comprises the following steps:

[0040] (1) The preparation method containing fluorescent quantum dots / silica composite microspheres was prepared according to existing literature (M.Y.Gao et al, J.Phys.Chem., 1998, 102, 8360; Y.J.Gong et al, Chem.Mater. , 2005,17,2648; Y.H.Yang et al, Adv.Mater., 2005,17,2354; L.H.Jing et al, Chem.Mater., 2010,22,420-427), the specific process of preparing fluorescent quantum dots is as follows : The cadmium salt (including cadmium perchlorate, cadmium chloride, cadmium acetate) is prepared into a solution between 0.001mol / L~0.15mol / L, preferably 0.005mol / L~0.05mol / L, and then adding a mercapto modifier (The molar ratio of cadmium salt to mercapto modifier is between 1:1~1:5, preferably between 1:1.0~1:2.5), and the pH value is adjusted in the range of 10~13, preferably 11.0~12.5. Then the tellurium ...

Embodiment 1

[0059] Weigh 0.631g cadmium perchlorate (Cd(ClO 4 ) 2 ·6H 2 O) Add to 75mL deaerated secondary deionized water, then add 0.182g mercaptoglycerol and 0.058g thioglycolic acid stabilizer, then adjust its pH value to 12.0 with NaOH aqueous solution to form a solution containing mercapto compounds and cadmium ions. On the other hand, inject 7.5mL of 0.5mol / L sulfuric acid solution into a container containing 0.129g of aluminum telluride (Al 2 Te 3 ) in the flask, the generated H 2 Put all Te into the above-mentioned cadmium ion solution, stir for 30 minutes, heat and reflux for 10 minutes to 10 days, and obtain a cadmium telluride fluorescent quantum that is co-stabilized with mercaptoglycerol and thioglycolic acid and whose fluorescence emission center peak is between 510 and 700 nanometers. Dot the water solution. Fluorescence spectrum see figure 1 .

Embodiment 2

[0061] Weigh 0.70g cadmium perchlorate (CdCl 2 2H 2 O) Add to 75mL of deaerated secondary deionized water, then add 0.185g of thioglycolic acid stabilizer, and then follow the Cd:S 2- =1:0.5 Add Na 2 S aqueous solution, and then use NaOH aqueous solution to adjust its pH value to 12.0, and heat the solution to reflux to obtain a water-soluble cadmium sulfide fluorescent quantum dot aqueous solution with stable thioglycolic acid and a fluorescent emission center peak position between 450 and 550 nanometers.

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Abstract

The invention relates to anti-bio-sorption surface-modified fluorescent quantum dot / silica composite microspheres, a preparation method thereof, and an application thereof. The method provided by the invention is characterized in that: fluorescent quantum dot / silica composite microspheres are first prepared with a reverse micro-emulsion method, and then biocompatible molecules and active functional groups are modified on the surfaces of the microspheres. The method is characterized by simple technology and convenient operation. The surface-modified fluorescent quantum dot / silica composite microspheres can overcome nonspecific interactions thereof upon biological molecules and cells, and can realize covalent coupling thereof with biological molecules. Therefore, surface-bio-functionalized molecular fluorescent probes are constructed. The fluorescent probes can be further applied in fields such as cell biology, bacteria detection, biological macromolecule detection, and immunofluorescence analysis.

Description

technical field [0001] The technical field of the present invention is material chemistry, nanoscience, surface chemistry, chemical biology and biomedicine, and particularly relates to a preparation method and biological application of fluorescent quantum dot / silicon dioxide composite microspheres that are resistant to biological adsorption. Specifically, biocompatible molecules and active functional groups are simultaneously modified on the surface of fluorescent quantum dots / silica composite microspheres by co-modification method, thereby constructing fluorescent quantum dots / silica fluorescent composites that are resistant to biosorption. The microspheres are then covalently coupled with biomolecules with different biological functions to obtain surface biofunctionalized fluorescent quantum dots / silica fluorescent composite microspheres that can resist biosorption. Background technique [0002] The unique optical properties of molecular fluorescent probes based on inorgan...

Claims

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Application Information

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IPC IPC(8): C09K11/88C09K11/59C12Q1/68G01N21/76G01N33/58
Inventor 高明远荆莉红乔瑞瑞
Owner INST OF CHEM CHINESE ACAD OF SCI
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