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Method for extracting hydrolyzed collagen from bovine cartilage

A technology for hydrolyzing collagen and cartilage, which is applied in the field of bioengineering, can solve problems such as poor molecular weight uniformity in the step-by-step range, lower biological activity, and affect product taste, so as to achieve the effects of ensuring excellent quality, increasing product content, and shortening process time

Active Publication Date: 2011-01-19
SHANGHAI AL AMIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, in the process of producing hydrolyzed collagen at home and abroad, most of the hydrolyzed collagen obtained by hydrolyzing animal bones, skins and other tissues by conventional methods has a wide molecular weight range and poor molecular weight uniformity; moreover, a large number of free amino acids produced during the hydrolysis process make the product The biological activity is greatly reduced, which not only affects the taste of the product, but also affects the efficacy

Method used

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  • Method for extracting hydrolyzed collagen from bovine cartilage

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Cut off fat and other sundries from the bovine cartilage taken out of the halal slaughterhouse, weigh 100kg, cut into small pieces, soak in 3% sodium hydroxide for 24 hours, and wash. Add 200kg of water, heat to 45°C, add papain at a ratio of 8500-9000U / g substrate, then add 0.03% M0111-9 compound enzymolysis protection agent, keep at 45-50°C, stir for 5 hours ; Then use sodium hydroxide to adjust the pH to 9.0-9.5, add 2709 alkaline protease according to the ratio of 5000U / g substrate, stir the enzymolysis at a constant temperature of 40-45°C for 3 hours, and inactivate the enzyme at 80-85°C For 10 minutes, cool to 30-40°C, add 3600ml (1.2% of the amount of feed liquid) of hydrogen peroxide, keep warm at 30-40°C for 2.5 hours; add 6kg of activated carbon to the feed liquid, stir for 2.5 hours, and use clarification The filter plate was filtered, and the obtained filtrate was subjected to ultrafiltration through a 200 Da nanofiltration membrane, and the retentate was sp...

Embodiment 2

[0039] Cut off fat and other sundries from the bovine cartilage taken out of the Muslim slaughterhouse, weigh 500kg, cut into small pieces, soak in 3% sodium hydroxide for 24 hours, and wash. Add 1000kg of water, heat to 45°C, add papain at a ratio of 8500-9000U / g substrate, then add 0.03% M0111-9 compound enzymolysis protection agent, keep at 45-50°C, stir for 5 hours ; Then use sodium hydroxide to adjust the pH to 9.0-9.5, add 2709 alkaline protease according to the ratio of 5000U / g substrate, stir the enzymolysis at a constant temperature of 40-45°C for 3 hours, and inactivate the enzyme at 80-85°C 10 minutes, cool to 30-40°C, add 18000ml (1.2% of the amount of feed liquid) of hydrogen peroxide, keep warm at 30-40°C for 2.5 hours; then add 30kg of activated carbon to the feed liquid, stir for 2.5 hours, Filtrate through a filter plate, and the obtained filtrate is subjected to ultrafiltration through a 200 Da nanofiltration membrane, and the retentate is spray-dried under t...

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Abstract

The invention provides a method for extracting hydrolyzed collagen from bovine cartilage. The method sequentially comprises the following steps of: processing fresh bovine cartilage which is obtained from a Muslim slaughter house and serves as a raw material with solution of sodium hydroxide; adding papain and alkali protease into the obtained product by stages and simultaneously adding a composite enzymolysis protecting agent into the obtained product; raising temperature for inactivating enzyme after two enzymolysis processes; decoloring by oxidation; filtering and performing ultra-filtering; collecting retained solution; and performing spray-drying on the solution to obtain the hydrolyzed collagen. In the method, the bovine cartilage of the Muslim slaughter house is used as a basic rawmaterial, and other animal derived enzyme preparations are not added during the entire process, so that the Muslim property and no pollution of the product can be ensured.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for preparing hydrolyzed collagen, in particular to a method for extracting hydrolyzed collagen from bovine cartilage. Background technique [0002] Collagen hydrolyzate is the most abundant animal protein in mammals. It mainly exists in animal bones, tendons, muscle sheaths, ligaments, sarcolemma, cartilage and skin. It is an extremely important type of connective tissue. Structural proteins play the role of supporting organs and protecting the body, and are also the most important functional proteins that make up the intercellular matrix. Collagen is composed of three peptide chains, two α(I) chains and one α(II) chain. These three peptide chains are intertwined with a unique left-handed α chain to form a right-handed complex helix of collagen. The characteristic is that the amino acid presents a chain Gly-x -Y periodic structure, extensive cross-linking between ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 苏翰苏有录唐甜
Owner SHANGHAI AL AMIN BIOTECH
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