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High-tolerance environment-friendly microbial modifier as well as preparation method and application thereof

A technology of tolerance and microorganisms, applied in the field of crack repair, can solve the problems of only wide and shallow cracks, limited application, and reduced urea hydrolysis rate, so as to achieve enhanced reinforcement or repair effects, avoid bacterial peeling, shorten The effect of reaction time

Active Publication Date: 2022-04-08
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the existing MICP repair technology is still limited to the indoor test stage, the main reason is that during the repair process, the bacteria are greatly affected by environmental factors, and it is difficult for deep micro-cracks, anoxic and unsuitable temperature, pH and other environments. All conditions will have an adverse effect on the bacteria, resulting in a greatly reduced urea hydrolysis rate and a reduced calcium carbonate deposition, thus affecting the repair effect of cracks
For example, Chinese patent CN111548040 discloses a preparation method and application method of a green microbial modifier for dispersible soil, which utilizes the intracellular urease solution extracted from the microbial bacterial liquid to react with urea and calcium sources, which can only be used for Wide and shallow cracks, and it cannot be used in alkaline environments with high pH
In addition, this method takes a long time to repair cracks, which greatly limits its application in practical engineering.

Method used

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  • High-tolerance environment-friendly microbial modifier as well as preparation method and application thereof
  • High-tolerance environment-friendly microbial modifier as well as preparation method and application thereof
  • High-tolerance environment-friendly microbial modifier as well as preparation method and application thereof

Examples

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preparation example Construction

[0028] see figure 1 , the present invention provides a kind of preparation method of the environment-friendly microorganism modifying agent of high tolerance, and this environment-friendly microorganism modifying agent of high tolerance comprises reagent A liquid and reagent B liquid, and described reagent A is centrifuged and purified, A mixed solution of enriched and cultured bacterial solution and a high-concentration urea solution, the reagent B is a mixed solution of nutrients required for bacterial metabolism, a high-concentration calcium source solution, and a low-concentration urea solution. The preparation method of the environment-friendly microbial modifier comprises the following S1-S6.

[0029] S1: Centrifuge the bacterial suspension after enrichment and culture of Bacillus pasteurianus or the previous generation of bacterial liquid to obtain bacterial sediment and bacterial supernatant.

[0030] The Bacillus pasteurianus in this example originated from the Ameri...

Embodiment 1

[0061] Step 1: Mix the bacterial liquid after centrifugal purification and enrichment culture treatment with the 2mol / L first concentration urea solution according to the volume ratio of 1:1, and cultivate for 24 hours at an ambient temperature of 30°C and an oscillation speed of 160rpm. Obtain reagent A solution.

[0062] Step 2: the calcium chloride solution with a concentration of 2mol / L and the second concentration urea solution with a concentration of 0.5mol / L are mixed according to a volume ratio of 1:1, and the initial pH of the mixed solution is adjusted to 6.0 using dilute hydrochloric acid to obtain Reagent B solution.

[0063] In this embodiment, the prepared microbial modifier is applied to figure 2 The concrete test block shown was used to test the formation of calcium carbonate crystals on the crack surface after using the microbial modifier to repair deep cracks. The specific steps of the test are as follows:

[0064] Step 1: Plug the cracks: use sandpaper t...

Embodiment 2

[0070] Step 1: Mix the bacterial liquid after centrifugal purification and enrichment culture treatment with 1.5mol / L first concentration urea solution according to the volume ratio of 1:1, and cultivate for 24 hours under the conditions of ambient temperature 30°C and shaking speed 160rpm , to obtain reagent A solution.

[0071] Step 2: mixing the calcium chloride solution with a concentration of 1.5 mol / L and the second concentration urea solution with a concentration of 1 mol / L at a volume ratio of 1:1, and using dilute hydrochloric acid to adjust the initial pH of the mixed solution to 6.0 to obtain Reagent B solution.

[0072] Using the corresponding application method in Example 1, the calcium carbonate coverage area of ​​the cracked surface of the repaired concrete block is 63% of the cracked surface area, and it is roughly in a uniform adhesion mode.

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Abstract

The invention belongs to the related technical field of crack repair, and discloses a high-tolerance environment-friendly microbial modifier and a preparation method and application thereof, and the method comprises the following steps: carrying out centrifugal treatment on a bacterial suspension obtained after enrichment culture of bacillus pasteurii or a last-generation bacterial liquid to obtain a bacterial precipitate and a bacterial supernatant; sequentially carrying out cell disruption treatment and centrifugal treatment on part of the bacterial precipitate to obtain cell disruption liquid; mixing the bacterial supernate, the other part of bacterial precipitate and the cell breaking liquid to obtain a bacterial enzyme mixture; inoculating the bacterial enzyme mixture into the liquid culture medium, and culturing to obtain bacterial liquid; mixing the bacterial liquid with first-concentration urea to obtain a reagent A liquid; and mixing the calcium source solution with the second-concentration urea to obtain a reagent B solution. The method can be applied to restoration of engineering bodies with severe microbial growth environments such as saline-alkali soil, the crack restoration effect is remarkably improved, and the method has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field related to crack repair, and more specifically relates to a high-tolerance environment-friendly microbial modifier and its preparation method and application. Background technique [0002] In recent years, the use of microbial-induced calcium carbide deposition (MICP) technology to repair material cracks to restore their damaged mechanical properties and durability has gradually become a major trend in crack repair technology. MICP technology cultivates specific microorganisms and provides sufficient nutrients and free calcium ions to generate calcium carbonate crystals with good cementing ability, so as to achieve the purpose of filling, cementing and repairing material cracks. [0003] At present, the research on the repair of material cracks using MICP technology is mainly focused on the passive repair of cracked materials, that is, after the material cracks are generated, the material environment require...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B41/62C04B41/45
Inventor 郑俊杰徐智韬陈一航蒋文松郑志善田聪曾晨
Owner HUAZHONG UNIV OF SCI & TECH
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