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Method for producing docosahexaenoic acid (DHA) through fermenting straw hydrolyzate

A docosahexaenoic acid and hydrolyzate technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem that the efficient and comprehensive utilization of straw resources cannot be truly realized, it is unfavorable for industrialization promotion, and the utilization rate is low and other problems, to achieve the effect of simple and easy fermentation process, low price and stable output

Active Publication Date: 2014-03-05
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Straw is a common lignocellulosic raw material in rural areas. At present, the common treatment methods for straw in rural areas in my country include incineration, composting, returning to the field, and making simple materials. The utilization rate is low and it is not conducive to the promotion of industrialization.
In addition, the comprehensive utilization methods of straw resources include direct fermentation to produce biogas, fermentation to produce fuel ethanol, lactic acid, etc., but the added value is low, and it is impossible to truly realize the efficient comprehensive utilization of straw resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, corn stalk hydrolyzate produces DHA

[0047] (1) Pretreatment of corn stalks by ammonia blasting method

[0048] The corn stalks were dried in an air blast oven at 80°C for about 2 days, ground with a straw grinder (Henan Hongyun Machinery Manufacturing Factory), and filtered through a mesh sieve with a pore size of 5 mm to obtain corn stalk powder. Take 100kg of dry straw powder, adjust the water content to 40% with distilled water, and place it in 1m 3 in the high pressure reactor. Take 100kg of ammonia water in a 200L stainless steel pressure tank, heat it to make the pressure reach 3.0MPa, and then pass it into a 1m tank filled with corn stalk powder. 3 high pressure reactor. Heat to 80°C with jacket steam and maintain for 60min. Then open the vent valve to allow the pressure in the high pressure reactor to drop. The straw powder pretreated with ammonia water was left overnight to allow the residual ammonia water to evaporate, and stored at 4°C fo...

Embodiment 2

[0060] Embodiment 2, rice straw hydrolyzate produces DHA

[0061] (1) Pretreatment of rice straw by ammonia blasting method

[0062] The rice straw was dried in an air blast oven at 80°C for about 2 days, ground with a straw grinder (Henan Hongyun Machinery Manufacturing Factory), and filtered through a mesh sieve with a pore size of 5 mm to obtain rice straw powder. Take 100kg of dry straw powder, adjust the water content to 45% with distilled water, and place it in 1m 3 in the high pressure reactor. Take 100kg of ammonia water in a 200L stainless steel pressure tank, heat it to make the pressure reach 3.5MPa, and then pass it into a 1m tank filled with rice straw powder. 3 high pressure reactor. Heat to 90°C with jacket steam and maintain for 45min. Then open the vent valve to allow the pressure in the high pressure reactor to drop. The straw powder pretreated with ammonia water was left overnight to allow the residual ammonia water to evaporate, and stored at 4°C for l...

Embodiment 3

[0074] Embodiment 3, sugarcane stalk hydrolyzate produces DHA

[0075] (1) Pretreatment of sugarcane straw by ammonia blasting method

[0076] The sugarcane straw was dried in an air blast oven at 80°C for about 2 days, ground with a straw grinder (Henan Hongyun Machinery Manufacturing Factory), and filtered through a mesh sieve with a pore size of 5 mm to obtain sugarcane straw powder. Take 100kg of dry straw powder, adjust the water content to 50% with distilled water, and place it in 1m 3 in the high pressure reactor. Take 100kg of ammonia water in a 200L stainless steel pressure tank, heat it to make the pressure reach 4.0MPa, and then pass it into a 1m tank filled with sugarcane straw powder. 3 high pressure reactor. Heat to 95°C with jacket steam and maintain for 30min. Then open the vent valve to allow the pressure in the high pressure reactor to drop. The straw powder pretreated with ammonia water was left overnight to allow the residual ammonia water to evaporate...

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Abstract

The invention discloses a method for producing docosahexaenoic acid (DHA) through fermenting a straw hydrolyzate. The method comprises the following steps of (1) pretreating straw powder by using an ammonia gas blasting method, so as to obtain pretreated straw powder; (2) carrying out enzymolysis on the pretreated straw powder by using cellulase, beta-galactosidase and pectinase, so as to obtain the straw hydrolyzate; (3) adding a culture medium into the straw hydrolyzate, then, inoculating Aurantiochytrium to carry out fermentation culture, so as to obtain Aurantiochytrium fermentation liquor; (4) extracting the Aurantiochytrium fermentation liquor by using mixed liquid of ethanol and n-hexane, so as to obtain an extract, and drying the extract, thereby obtaining the DHA. According to the DHA prepared by the method, the yield is stable; in the case of not adding exogenous glucose, by adopting the method, the dry weight of obtained thalli can reach 58-66g / L, oil and fat account for 40.8-53.3% of the dry weight of cells, the DHA accounts for 42.1-45.3% of the dry weight of the oil and fat, and the DHA yield is 10.3-15.4g / L.

Description

technical field [0001] The invention relates to a method for producing docosahexaenoic acid by fermenting straw hydrolyzate. Background technique [0002] Docosahexaenoic acid (DHA for short) is a long-chain highly unsaturated fatty acid necessary for the human body. It has significant anti-cardiovascular disease, lowering blood fat, lowering blood pressure, preventing various cancers, and promoting infant intelligence Development, improving the brain function of adults and preventing senile dementia and other important physiological functions have been developed into various medicines, health care products, high-grade cosmetics, food and feed, etc., with a huge market. The traditional resource of DHA is deep-sea fish oil, but due to overexploitation of deep-sea fish, fish oil resources are difficult to meet people's growing demand for DHA. In recent years, the use of microbial fermentation to produce oil rich in DHA has become a hot topic. The marine fungus Schizochytrium...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/645
Inventor 黄建忠江贤章张明亮
Owner FUJIAN NORMAL UNIV
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