84results about How to "Does not destroy biological activity" patented technology

This invention provides a method for preparing phycocyanin and allophycocyanin simultaneously. The method comprises: (1) preparing crude extract of phycocyanin; (2) filling a column with hydroxyapatite, and balancing the chromatographic column; (3) eluting phycocyanin and allophycocyanin, respectively; (4) concentrating, desalting and freeze-drying the eluate to obtain phycocyanin and allophycocyanin. The method utilizes powder of artificially bred Spirulina platensis as the raw material, which can be purchased in a large quantity, and has low cost and high purity. Besides, the method adopts hydroxyapatite column chromatography to separate phycocyanin and allophycocyanin, thus simplifying the preparation process. Hydroxyapatite can be easily prepared, thus lowering the cost. The method is ideal for preparing phycocyanin and allophycocyanin simultaneously.

The invention relates to a triad integration freckle-removing functional nano cream for skin external use and a method for preparing the same. The three types (night use type, daytime use type and auxiliary type) freckle-removing nano creams improve and optimize a novel international passive target transdermal drug delivery system (TDDS) as a carrier, and organically combine and solubilize freckle-removing functional components with different biological efficacies, action ways and physical and chemical properties to form a good mutual aid synergic function system which has remarkable effect of controlling and relieving colored patches (such as chloasma, inflammatory pigmentation, sunburn, and the like) caused by abnormal facial metabolism of pigment. The product has the advantages of tiny particle sizes of spherical particles, obvious passive target function, excellent adaptability, homodromous property, fluidity and dispersion, strong penetrability, even texture, subtleness, lubricity and luster, is simple and convenient to use, and is comfortable without pungency and side effects; besides, the appearance is light yellow with slight opalescence, is glittering and translucent, clear and transparent, sticky but not viscous, thick but not dense, and oily but not greasy, has good stability, excellent curative effect, short industrialization cycle and high production efficiency, saves time, materials and energy sources, dose not need special devices and heat energy, and is easy for popularization and application.

The invention discloses a bone growth factor controlled release type bone repairing material as well as a preparation method and applications thereof. The bone repairing material provided by the invention is characterized in that the pore surface of a porous bone repairing inorganic material is coated with two layers of membranes from inside to outside, wherein the first-layer membrane is mainly composed of a bone growth factor and chitosan; and the second-layer membrane is formed by mixing a bone growth factor with biodegradability poly anions at a mass ratio of (0-0.001):1. The preparation method provided by the invention comprises the following steps: soaking the porous bone repairing inorganic material into a bone growth factor/chitosan solution, and airing; and soaking in a bone growth factor/biodegradability poly anion aqueous solution, and airing to obtain the bone growth factor controlled release type bone repairing material. The preparation method provided by the invention is simple; and the obtained bone repairing material has good mechanical property and biocompatibility, and ensures that the continuous slow united release or sequential release of various bone growth factors can be realized, and the repairing effect of a bone defect tissue is improved, thereby having a good clinical application prospect in bone repairing.

The invention relates to a new traditional Chinese medicine effective components extraction technique, in particular to a new auxiliary method to effectively extract total flavonoids from ginkgo biloba via ultrasonic. The invention adopts orthogonal experiment design scheme and is based on technical parameters like ultrasonic occurring mode in screening extraction process, ultrahigh ultrasonic intensity, rapid action time and mode, solvents, system temperature and stirring method, etc, to prepare fresh ginkgo leaf tablets; the tablets are baked for 24 hours under conditions of 60 degrees centigrade, sieved (0.45 Mu m) to produce raw material; the raw material is put in ultrasonic extraction pot and is added with alcohol with 60 percent alcohol, with a ratio of material to liquid (Kg:L)1:15 to 17, and is mixed. The ultrasonic occurring mode is: separately stricken ultrasonic sound generating mechanism, radial vibration columnar transducer; the ultrasonic power is: 100W; the ultrasonic frequency is 33kHz;the distilling way is: ultrasonic function every two minutes for 3 minutes, with the total extraction time 25 minutes; the extraction temperature is 55 degrees centigrade; and the stirring mode is air-lift stirring. Prepared in the above method, the extraction rate of the ginkgo biloba total flavonoides is as high as 97.35 percent.

The invention discloses a preparation process of efficient humic acid diammonium phosphate, which belongs to the field of novel fertilizers and in particular relates to a production process for preparing an efficient humic acid diammonium phosphate product through modifying diammonium phosphate in the traditional method. The preparation process comprises the following steps of: (1) utilizing 30-50wt% of humic acid ammonium containing water soluble humic acid as an activating agent, and adding the powder or paste humic acid ammonium to a returning charge according to 10-20wt% of adding amount in the returning charge part of the course of producing diammonium phosphate by adopting the traditional method; and (2) uniformly mixing the diammonium phosphate returning charge containing the added humic acid ammonium, then adding the diammonium phosphate returning charge into a cylinder for pelletizing, pelletizing and aminating the diammonium phosphate returning charge together with pre-neutralizing slurry, and drying. The preparation process of the efficient humic acid diammonium phosphate has the advantages that because the humic acid ammonium containing high water soluble humic acid is added in the returning charge part in the production process of the diammonium phosphate by utilizing the traditional method, the physiological activity of the humic acid is not destroyed, the traditional production process of the diammonium phosphate is not affected, the production cost of the diammonium phosphate can be effectively reduced, and the obtained efficient humic acid diammonium phosphate has excellent agricultural effect and ecological effect.

The invention relates to a novel technology for extracting the effective component of the traditional Chinese medicine and concretely relates to a novel technical method of ultrasonic extraction, which is to high efficiently extract the effective component of paeoniflorin form the compound traditional Chinese medicines of a honeysuckle, a red peony root and a male fern rhizome. The method adopts the design scheme of orthogonal experiment to obtain the dry honeysuckle, red peony root and male fern rhizome with the weight ratio of 3 to 3 to 1 by the filtration and ultrasonic extraction process based on the technical parameters of the ultrasonic intensity, the ultrasonic acting time and acting manner, the solvent type, the system temperature, the mixing manner etc., and the dry honeysuckle, red peony root and male fern rhizome are crushed and sieved (0.45 microns) as the material to be mixed with water, and the material-liquid volume ratio is 1 to 5 to 7. The ultrasonic power is 100W; the ultrasonic frequency is 25kHz; the ultrasonic acting manner is a separate excitation type ultrasonic vocalization device and a columnar radial-vibration transducer; the ultrasonic acting time is 40 minutes; the extraction temperature is 45 DEG C; the mixing manner is the gas lift-type mixing. The extracted liquid is filtered and condensed for the comparative consistency of 1.08 to 1.16, and ethanol is added till the alcohol content is 50 percent, and the liquid is mixed uniformly to be aged overnight and filtered; the filtrate is regenerated the ethanol and condensed; medical powder granules are obtained by spraying and drying at the extract flow velocity of 4 to 6 ml/min and the atomizing pressure of 1 to 2 kg. According to the method, the yield of the medical powder granular matter is 32 percent; the content of the paeoniflorin is 5.92 percent.

This invention relates to a method preparing phycocyanin a method of extracting phycocyanin from blue-green alga. This method character in that it has low cost, easy operation way, high quality and quantity compared with traditional way, is also has a strongpoint of high purity, and bioactivity of protein, is prone to a industrial production of phycocyanin.

The invention discloses an antibacterial treatment method of a silicon surface. The method comprises the following steps: (1) treating a Si (100) surface of a silicon-based material by virtue of hydrofluoric acid; (2) dissolving silane coupler by taking ethanol as solvent, and obtaining silane coupoler solution; (3), adding a click reaction solution containing antibacterial polypeptide into the silane coupler solution in the step (2), and carrying out the click chemical reaction; (4) spinning drying the solution after the reaction of the step (3), adding a mixed solution of water and ethanol, and re-dissolving a product to obtain a solution; (5) dropwise adding the solution obtained in the step (4) onto the Si (100) surface, carrying out the curing treatment to obtain a silicon-based material with the antibacterial surface. By adopting the method, the antibacterial performance of the silicon material is remarkably improved, and a prepared Si (100) substrate has low biological toxicity.

The invention discloses a medical hemostasis antibacterial dressing. The medical hemostasis antibacterial dressing is prepared from the raw materials in parts by weight: 60 to 80 parts of polyglutamicacid-Pulullan cross-linked polymer, 25 to 35 parts of collagen, 13 to 17 parts of bamboo vinegar powder, 12 to 18 parts of glycollic acid, 6 to 10 parts of garlic polysaccharide, 5 to 15 parts of hyaluronic acid, and 50 to 60 parts of pure water. The invention also discloses a preparation method of the medical hemostasis antibacterial dressing. The medical hemostasis antibacterial dressing of theinvention has excellent performance for covering wounds, helping the wound healing, efficiently stopping bleeding, preventing the bacterial invasion, promoting the tissue restoration and preventing the adhesion, can rapidly absorb the water in the blood when contacting the wound, can keeping the environment wet, and can effectively seal the bleeding wound.

The invention relates to a method for extracting plant oil from plants, in particular to taxus chinensis oil and an extracting method thereof. The extracting method comprises the following steps that seeds of taxus chinensis fruit are taken as raw materials, oil is obtained through extraction by adopting a microwave method and a solvent method, residual solvent odor and impurities are removed through a supercritical CO2 method, standing, refined filtering and refining are performed on a re-extraction product under the low temperature condition, and then the taxus chinensis oil is obtained. According to the method, re-extracting and refining are performed by adopting the microwave-assistant method and the solvent method to be combined with supercritical CO2 technology equipment, and therefore not only is the basic characteristic that the biological activity is not destroyed under the low temperature condition guaranteed, but also the residual solvent odor and the impurities are removed more effectively and the high standard of the green quality is achieved.

The present invention relates to an extraction technology of effective components in Chinese medicine. Specifically, the present invention relates to a novel method of using ultrasound to extract an effective component quercetin in ginkgo leaves. The method uses a design program of orthogonal experiment. Based on technical parameters of the generation method, ultrasonic intensity, functional duration, functional method, solvent type, temperature of the system, and the mixing method of ultrasound through screening and extraction process, the fresh ginkgo leaves can be prepared. The ginkgo leaves are dried at a temperature of 60 DEG C for 24 hours and crushed and screened (0.45 Mu m) to be a material. The ginkgo leaves are filled into ethanol solution of 45 percent in an ultrasonic extraction reactor; the material and solution are mixed according to a ratio (Kg: L) of 1 to from 14 to 15. The generation method of ultrasound comprises a simulated audible device of ultrasound and a radial cylindrical vibration transducer. The ultrasonic power is 100W, and the ultrasonic frequency is 28 kHz. In the extraction method, the ultrasound functions once with an interval of 5 minutes; the functional time of ultrasound is 3 minutes; the total extraction time is 32 minutes; the extraction temperature is 45 DEG C; the stirring method is an air-lift stirring way. With the methods, the extraction rate of the effective component quercetin in ginkgo leaves can reach 96.28 percent.

The present invention relates to a novel extraction technology of effective components in Chinese medicine. Specifically, the present invention relates to a novel method of using ultrasound to extract an effective component isorhamnetin in ginkgo leaves. The method uses a design program of orthogonal experiment. Based on technical parameters of the generation method, ultrasonic intensity, functional duration, functional method, solvent type, temperature of the system, and the mixing method of ultrasound through screening and extraction process, the fresh ginkgo leaves can be prepared. The ginkgo leaves are dried at a temperature of 60 DEG C for 24 hours and crushed and screened (0.45 Mu m) to be a material. The ginkgo leaves are filled into ethanol solution of 45 percent in an ultrasonic extraction reactor; the material and solution are mixed according to a ratio (Kg: L) of 1 to from 17 to 19. The generation method of ultrasound comprises a simulated audible device of ultrasound and a radial cylindrical vibration transducer. The ultrasonic power is 100W, and the ultrasonic frequency is 28 kHz. In the extraction method, the ultrasound functions once with an interval of 3 minutes; the functional time of ultrasound is 3 minutes; the total extraction time is 36 minutes; the extraction temperature is 45 DEG C; the stirring method is an air-lift stirring way; the content of isorhamnetin in the extraction solution is analyzed by using high-efficiency liquid-phase chromatogram and the quantity is fixed in a standard-material external marking way. With the methods, the extraction rate of the effective component isorhamnetin in ginkgo leaves can reach 96.77 percent.

The invention discloses a pomelo seed polysaccharide extraction process which comprises the steps: drying pomelo seeds, crushing to obtain pomelo seed powder, carrying out degreasing treatment on the pomelo seed powder, carrying out water extraction combined with an enzyme method, simultaneously carrying out ultrasonic extraction to obtain seed polysaccharide, and sequentially carrying out deproteinization and desalination treatment on the obtained crude polysaccharide, finally obtaining purified pomelo seed polysaccharide through ion exchange chromatography and molecular sieve chromatography. According to the process, degreasing pretreatment, hot water extraction, enzyme extraction and ultrasonic extraction are adopted so that the extraction time and cost of the pomelo seed polysaccharide are greatly reduced, and the extraction rate is increased. According to the process the grapefruit seeds are used as a new polysaccharide resource, so that the process has a wide utilization prospect, and has a positive promotion effect on deep processing of the grapefruit grains and development of the grapefruit industry.

The invention provides an ultrasonic-assisted method for extracting total flavonoid in alfalfa.The method comprises the following steps: (1) alfalfa is dried and smashed to obtain alfalfa powder; (2) an organic solvent (such as petroleum ether) is added to the alfalfa powder obtained in the step (1), heating reflux is performed for 4-24 hours, filtering is performed so as to remove the organic solvent, the alfalfa powder is obtained, and then the alfalfa powder is dried; (3) an ethanol solution with the volume fraction ranging from 20% to 70% and the alfalfa powder obtained in the step (1) are mixed according to the liquid-to-solid ratio of mL:g ranging from (20:1) to (70:1), extraction is performed at the temperature of 30 DEG C to 80 DEG C and under ultrasonic assistance, and an alfalfa total flavonoid extraction solution is obtained.The extraction rate of the total flavonoid can be improved with the method, the ultrasonic extraction is a physical process, the optimum temperature of the extraction process is about 60 DEG C, active ingredients of the flavonoid material in the alfalfa can be prevented from being changed by high temperature, and the extraction efficiency of the total flavonoid in the alfalfa is improved.

The invention provides a method for separating and purifying 5'-cytidylic acid and derivatives thereof. The nanofiltration membrane separation technology is applied to the separation and purificationof the 5'-cytidylic acid and the derivatives thereof, and the separating and purifying process comprises desalting operation and concentrating operation. The method has the advantages of no phase change, no chemical reaction, no damage to bioactivity, no need of heating and normal-temperature operation in the separating process, simple steps, high selectivity, low energy consumption and the like.

The invention discloses a dielectrophoresis-based micro-fluidic chip for separation of deformable microparticles. The dielectrophoresis-based micro-fluidic chip is formed by connection of a primary pipeline and a secondary pipeline, wherein an electric field is arranged in the primary pipeline and can drive deformable microparticles to move towards the secondary pipeline, and the secondary pipeline is divided into an upper pipeline and a lower pipeline and used as a separation pipeline for the microparticles after movement. To-be-separated microparticles are allowed to move towards secondary pipelines located at different directions after passing through a contraction structure by setting different parameters like electric field intensity and the radius of a contraction opening. The dielectrophoresis-based micro-fluidic chip provided by the invention has the advantages that the deformable microparticles may be biological cells, and the biological properties and activity of the biological cells are not damaged in the process of separation; through setting of different parameters, particles with radiuses in a certain range can be perfectly separated; a separation structure is simple; separation process is good in operability; a separation channel is short; separation time is short; and separation efficiency is high.

The invention relates to an extraction method for tea leaves, in particular to a method for extracting active ingredients from Camellia chrysantha leaves. The method comprises the following steps: the Camellia chrysantha leaves are collected and washed with clear water; the Camellia chrysantha leaves are cut into small leaf strips by a herbal medicine slicing machine, the small leaf strips are mashed by a mashing machine, and Camellia chrysantha pulp is obtained; the Camellia chrysantha pulp is transferred into a multifunctional stainless steel extraction tank, and pectinase is added to the stainless steel extraction tank; heating sterilization is performed; ethyl alcohol is added, an ultrasonic extraction unit is started for oscillating extraction, filtering is performed after extraction, and residues and a primary filtrate are obtained; water and ethyl alcohol are added to the residues, the mixture is subjected to secondary ultrasonic oscillating extraction, filtering is performed, and a secondary filtrate is obtained; the filtrates are filtered by a 200-mesh plate and frame filter; the primary filtrate and the secondary filtrate are concentrated by a vacuum drier, and a concentrated Camellia chrysantha solution containing the active ingredients is obtained. The extraction method cannot damage biological activity and adopts simple process and normal-temperature concentration, so that loss of physiologically active ingredients is reduced, energy consumption is reduced, and extraction rate is high.

The invention discloses a polyurethane-based cellular material compounding vessel growth promotion factors and a preparation method of the polyurethane-based cellular material. The polyurethane-based cellular material is obtained by arranging a polyurethane-based cellular material compounding glycosaminoglycan in a phosphate buffered solution containing 0.1 to 3 microgram/mL of the vessel growth promotion factors, soaking for 1 to 120 minutes under the temperature of 4 to 37 DEG C, then repeatedly rinsing for multiple times with the phosphate buffered solution, and freeze-drying, wherein each rinsing lasts for 1 to 120 minutes. The polyurethane-based cellular material compounding the vessel growth promotion factors has the advantages of having good air permeability and hygroscopicity, promoting wound vessel regeneration and skin tissue repair, reducing scar formation and the like.