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Simplified method for extracting a great lot phycocyanin

A technology of phycocyanin and cyanobacteria, which is applied in the field of extracting phycocyanin, can solve the problems of high price of phycocyanin products and no process methods, etc., and achieve the effect of simple operation, low cost and high purity

Inactive Publication Date: 2005-10-26
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is mainly because the extraction of phycocyanin from blue-green algae and blue-green algae is still in the laboratory research stage, and there is no good process method suitable for industrial production, which makes the commodity price of phycocyanin expensive and limited in application. 【8】

Method used

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  • Simplified method for extracting a great lot phycocyanin
  • Simplified method for extracting a great lot phycocyanin
  • Simplified method for extracting a great lot phycocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Extraction of phycocyanin from fresh algae using bacterial culture solution

[0018] A) Spirulina culture

[0019] Spirulina is inoculated in Zarrouk medium [12], and this medium comprises (g / l): NaHCO 3 16.8, NaNO 3 2.5,K 2 HPO 4 0.5,K 2 SO 4 1.0, NaCl 1.0, MgSO 4 ·7H 2 O 0.2, CaCl 2 0.04, FeSO 4 7H 2 O 0.01, EDTA 0.08 and trace elements. Shaking culture at 130rpm / min, constant temperature at 30°C, 100μmol / m 2 / s continuous light, culture until the OD value A560 of the algae solution reaches about 1.2, filter through a filter with a pore size of 0.45 μm to obtain fresh algae mud, and use it in the following steps without cleaning.

[0020] B) Anabaena and Synechocystis culture

[0021] Inoculate Anabaena sp.595 and Synechocystis PCC6803 (algae species from the Institute of Hydrobiology, Chinese Academy of Sciences) on BG-11 medium, see literature for ingredients 【13】 Medium, (g / l): NaNO 3 , 1.5; K 2 HPO 4 ·3H 2 O, 0.04; MgSO 4 ·7H 2 O, ...

Embodiment 2

[0039] Example 2 Extraction of phycocyanin in spirulina algae powder using bacterial culture solution

[0040] Extraction of phycocyanin was performed in the same manner as in Example 1. The difference is that algal powder is used instead of fresh algae. The specific steps are as follows: Weigh 500 g of 100% algae powder of Yunnan-produced Xiangfeng brand (Beijing Sunshine Yuhongfeng Product Technology Development Company) and place it in a container, add 5000 ml of various bacterial liquids cultivated in Example 1 to mix, and leave it at room temperature At about 25°C, after half an hour, phycocyanin precipitates, and the supernatant is clear and blue, and the supernatant is poured out. Remaining steps are identical with embodiment 1, obtain similar result. It should be noted that the purity of the phycocyanin crude extract obtained by using algae powder is not as high as that obtained by using fresh algae mud, but the precipitation time of phycocyanin is doubled. The stru...

Embodiment 3

[0041] Embodiment 3 Comparison of different crude extraction methods of phycocyanin

[0042] In order to compare the extraction method of the method of the present invention and conventional phycocyanin, fresh algal mud and / or algae powder are extracted according to various phycocyanin extraction methods recorded in the literature under control conditions, and the results are shown in Table 1 .

[0043] Table 1 Comparison chart of phycocyanin in fresh algae mud (spirulina) crudely extracted by different methods

[0044] Extraction method Phycocyanin concentration Phycocyanin purity Scale and equipment

[0045] (μg / ml) (A 620 / A 280 )

[0046] Ultrasonic crushing at 4°C 300±12 0.30 Strict equipment requirements

[0047] grid, small amount each time

[0048] Difficult to control temperature

[0049] Pressure at room temperature 25°C 369±15 0.40 Special eq...

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Abstract

This invention relates to a method preparing phycocyanin a method of extracting phycocyanin from blue-green alga. This method character in that it has low cost, easy operation way, high quality and quantity compared with traditional way, is also has a strongpoint of high purity, and bioactivity of protein, is prone to a industrial production of phycocyanin.

Description

technical field [0001] The invention relates to a method for biologically extracting phycocyanin, more specifically, the invention relates to a method for extracting phycocyanin from blue-green algae and blue-green algae with a culture solution of microorganisms. Background technique [0002] Phycocyanin has high development and utilization value 【1】 . First of all, phycocyanin is a unique photosynthetic pigment of red algae and cyanobacteria. It has bright and bright color. It is the first choice of pure natural pigment for food, high-end eye shadow and lipstick. It can be used as a pure natural pigment for food, cosmetics and dye industries. 【2】 . Then it can be processed into health food and medicine 【3】 . Studies have shown that phycocyanin can significantly reduce and gradually eliminate the damage to hematopoietic function caused by radiation and chemotherapy 【4】 , can also improve the activity of lymphocytes, and actively remove oxygen free radicals in cells 【5】...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 朱毅李永兴王可玢白克智匡廷云
Owner INST OF BOTANY CHINESE ACAD OF SCI
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