Method for preparing quick magnetic separation electrochemistry immunosensor and method for detecting staphylococcus aureus
An immunosensing and staphylococcus technology, applied in the field of biosensing, can solve the problems of high dependence on automated instruments and high detection costs, and achieve the effects of good practicability, strong biological affinity and good specificity
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[0030] The present invention is described in further detail below in conjunction with accompanying drawing embodiment, and concrete operation method is as follows:
[0031] (1) Preparation of CdTe quantum dots
[0032] 26 μL of 6 mM mercaptopropionic acid (MPA) was added to 50 mL of 2 mM CdCl 2 In the solution, the pH was adjusted to 9.0 with 1M NaOH solution, and N 2 Mix for 30 minutes, then slowly add 0.8mL of 0.0625M NaHTe solution to form dark yellow CdTe quantum dots. Cd 2+ / MPA / The - The material mass ratio is 1:3:0.5. The obtained quantum dot solution was refluxed for 10 hours, the attainable particle size was 2.6 nm, and the concentration was 4.7 μM. The prepared quantum dot solution was ultrafiltered at 15,000 g for 10 min at 4°C to remove excess mercaptopropionic acid, rinsed twice, and finally adjusted to 700 μL with PBS with pH 7.4, and placed in a refrigerator at 4°C save.
[0033] (2) Functionalization of CdTe quantum dots
[0034] The cross-linking reage...
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