Method for abstracting active ingredient of Isorhamnetin in folium ginkgo with ultrasound wave

Abstract

The present invention relates to a novel extraction technology of effective components in Chinese medicine. Specifically, the present invention relates to a novel method of using ultrasound to extract an effective component isorhamnetin in ginkgo leaves. The method uses a design program of orthogonal experiment. Based on technical parameters of the generation method, ultrasonic intensity, functional duration, functional method, solvent type, temperature of the system, and the mixing method of ultrasound through screening and extraction process, the fresh ginkgo leaves can be prepared. The ginkgo leaves are dried at a temperature of 60 DEG C for 24 hours and crushed and screened (0.45 Mu m) to be a material. The ginkgo leaves are filled into ethanol solution of 45 percent in an ultrasonic extraction reactor; the material and solution are mixed according to a ratio (Kg: L) of 1 to from 17 to 19. The generation method of ultrasound comprises a simulated audible device of ultrasound and a radial cylindrical vibration transducer. The ultrasonic power is 100W, and the ultrasonic frequency is 28 kHz. In the extraction method, the ultrasound functions once with an interval of 3 minutes; the functional time of ultrasound is 3 minutes; the total extraction time is 36 minutes; the extraction temperature is 45 DEG C; the stirring method is an air-lift stirring way; the content of isorhamnetin in the extraction solution is analyzed by using high-efficiency liquid-phase chromatogram and the quantity is fixed in a standard-material external marking way. With the methods, the extraction rate of the effective component isorhamnetin in ginkgo leaves can reach 96.77 percent.

Description

1. Technical field

[0001] The invention relates to a new technology for extracting effective components of traditional Chinese medicine, in particular to a new method for extracting isorhamnetin, an effective component in ginkgo leaves, by ultrasonic waves. 2. Background technology

[0002] The chemical meaning of extraction is to use a suitable solvent and an appropriate method to leach the required components from the sample material as completely as possible, avoiding or reducing the introduction of harmful components or other impurities. Extracting chemical components from plants for medicinal purposes has a history of nearly 5,000 years in the traditional medicine of the motherland. Traditional extraction methods can be divided into two categories according to the different energy requirements of the extraction process. One is the heating extraction method, which mainly includes different methods such as decoction extraction, reflux extraction and Soxhlet extraction; t...

Examples

Embodiment Construction

[0013] The best technical solutions obtained are:

[0014] Fresh ginkgo leaves were dried at 60°C for 24 hours, crushed, and sieved (0.45 μm) as the material. Put it into an ultrasonic extraction kettle, add 45% ethanol, the ratio of material to liquid (Kg:L) is 1:17-19, and mix. Ultrasonic generation method: other excitation ultrasonic sounding device, radial vibration columnar transducer; ultrasonic power: 100W; ultrasonic frequency: 28kHz; extraction method: interval 3min, ultrasonic action once, ultrasonic action time 3min, total extraction time 36min; extraction Temperature: 45°C; stirring method: air-lift stirring; the content of isorhamnetin in the extract was analyzed by high-performance liquid chromatography and quantified by the external standard method of standard substances. According to the above method, the extraction rate of isorhamnetin, the active ingredient in ginkgo leaves, can reach 96.77%.

1. Technical field

[0001] The invention relates to a new technology for extracting effective components of traditional Chinese medicine, in particular to a new method for extracting isorhamnetin, an effective component in ginkgo leaves, by ultrasonic waves. 2. Background technology

[0002] The chemical meaning of extraction is to use a suitable solvent and an appropriate method to leach the required components from the sample material as completely as possible, avoiding or reducing the introduction of harmful components or other impurities. Extracting chemical components from plants for medicinal purposes has a history of nearly 5,000 years in the traditional medicine of the motherland. Traditional extraction methods can be divided into two categories according to the different energy requirements of the extraction process. One is the heating extraction method, which mainly includes different methods such as decoction extraction, reflux extraction and Soxhlet extraction; t...

Examples

Embodiment Construction

[0013] The best technical solutions obtained are:

[0014] Fresh ginkgo leaves were dried at 60°C for 24 hours, crushed, and sieved (0.45 μm) as the material. Put it into an ultrasonic extraction kettle, add 45% ethanol, the ratio of material to liquid (Kg:L) is 1:17-19, and mix. Ultrasonic generation method: other excitation ultrasonic sounding device, radial vibration columnar transducer; ultrasonic power: 100W; ultrasonic frequency: 28kHz; extraction method: interval 3min, ultrasonic action once, ultrasonic action time 3min, total extraction time 36min; extraction Temperature: 45°C; stirring method: air-lift stirring; the content of isorhamnetin in the extract was analyzed by high-performance liquid chromatography and quantified by the external standard method of standard substances. According to the above method, the extraction rate of isorhamnetin, the active ingredient in ginkgo leaves, can reach 96.77%.

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