The present invention relates to a novel extraction technology of effective components in Chinese
medicine. Specifically, the present invention relates to a novel method of using
ultrasound to extract an effective component
isorhamnetin in
ginkgo leaves. The method uses a design program of orthogonal experiment. Based on technical parameters of the generation method, ultrasonic intensity, functional duration,
functional method,
solvent type, temperature of the
system, and the mixing method of
ultrasound through screening and extraction process, the fresh
ginkgo leaves can be prepared. The
ginkgo leaves are dried at a temperature of 60 DEG C for 24 hours and crushed and screened (0.45 Mu m) to be a material. The ginkgo leaves are filled into
ethanol solution of 45 percent in an ultrasonic extraction reactor; the material and solution are mixed according to a ratio (Kg: L) of 1 to from 17 to 19. The generation method of
ultrasound comprises a simulated audible device of ultrasound and a radial cylindrical vibration
transducer. The ultrasonic power is 100W, and the ultrasonic frequency is 28 kHz. In the extraction method, the ultrasound functions once with an interval of 3 minutes; the functional time of ultrasound is 3 minutes; the total extraction time is 36 minutes; the extraction temperature is 45 DEG C; the stirring method is an air-lift stirring way; the content of
isorhamnetin in the extraction solution is analyzed by using high-efficiency liquid-phase chromatogram and the quantity is fixed in a standard-material external marking way. With the methods, the extraction rate of the effective component
isorhamnetin in ginkgo leaves can reach 96.77 percent.