Latex dispersion preparation method and kit

A technology of dispersion and latex, which is applied in the field of dispersion of latex for protein carriers, can solve the problems of low production efficiency and long production time, and achieve the effects of increased production capacity, good detection sensitivity and improved working environment

Active Publication Date: 2019-09-20
SHANGHAI UPPER BIO TECH PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in order to solve the problems of long production time and low production efficiency, there is also a need to provide a new method of dispersing latex to obtain products with good performance and enlarged production capacity, and at the same time, it can process a large number of products in a short time to save time and cost , but also improve the working environment

Method used

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  • Latex dispersion preparation method and kit
  • Latex dispersion preparation method and kit
  • Latex dispersion preparation method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] 1) Preparation of latex precipitate:

[0103] The latex obtained by styrene-based polymerization is activated with NHS and EDC, and then chemically coupled with the corresponding antibody after the treatment. The latex conjugate with large particles was collected, and the latex solution was processed by centrifugation to obtain about 10 L of latex precipitate.

[0104] 2) The latex sediment is broken up:

[0105] The latex precipitate in 1) is equally divided into two parts (0.5 L per part), one part adopts the current technology (magnetic stirring or manual milling) to process, and the other part adopts the high-speed shearing machine of the present invention to carry out mechanical milling. With the same processing capacity, the present invention breaks up the processing time for about 5 to 6 minutes, while the prior art requires at least 2 hours for processing. After processing, it is large particle latex.

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Abstract

The invention relates to a latex dispersion preparation method and a kit. The method is a method for preparing the dispersion of polymer latex loaded with a bioactive substance. The method comprises the steps that a) the latex is mechanically milled in a high-speed shear to acquire the dispersion containing large particle latex particles, wherein the average particle diameter of the large particle latex particles is 50 to 1000 microns; and b) the acquired dispersion containing the large particle latex particles is homogenized in a high-pressure homogenizer at 0 to 30 DEG C to acquire the latex dispersion, wherein the average particle diameter of latex particles in the latex dispersion is 50 to 500 nm. The latex dispersion preparation method provided by the invention has the advantages of short time, high efficiency and the like, can acquire products with good performance and enlarged capacity, and at the same time improves a working environment.

Description

technical field [0001] The invention belongs to the field of clinical medical diagnostic products, in particular to a method for dispersing latex, especially protein carrier latex used in the field of point of care testing (POCT). Background technique [0002] Currently, latex dispersions based on bioactive substances have been widely used. Through various modification or loading means, specific functional substances, such as antibodies, are loaded on the particle surface of the latex dispersion to achieve various detection applications. Typically, such biologically active latex dispersions can be used in kit-type assays. [0003] Generally, the material of latex can be particles formed from various organic polymers, and biologically active substances are loaded on the surface by chemical and / or physical means. For example, currently in the field of POCT (point-of-care detection), latex and NHS (N-hydroxysuccinimide), EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543C08J3/02C08L25/06
CPCG01N33/531G01N33/54313C08J3/02C08J2325/06
Inventor 邹勇辉龚杰范宏周
Owner SHANGHAI UPPER BIO TECH PHARMA
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