Deproteinized calf blood extract composition as well as injection and preparation method thereof

Abstract

The invention discloses a method for preparing a deproteinized calf blood extract. The method comprises the following steps: (a) performing negative catalysis; (b) performing acid precipitation; (c) freezing at a low temperature, raising the temperature, dissolving, centrifuging, and taking the supernatant; (d) performing alkali precipitation; (e) repeating the step (c); (f) performing alcohol precipitation and centrifuging the supernatant in the step (e), and taking the supernatant; (g) performing ultrafiltration and vacuum concentration on the supernatant in the step (f), thereby obtaining concentrated ultrafiltrate; and (h) performing ultrafiltration on the concentrated ultrafiltrate in the step (g), collecting the ultrafiltrate, thereby obtaining the product. According to the preparation method, the biological activity is not damaged, exogenous ions are not introduced, and hybrid proteins in the extract can be effectively removed. The injection prepared from the deproteinized calf blood extract does not cause anaphylactic reaction, is high in safety, and can effectively treat brain cell metabolic disorder diseases such as cerebral apoplexy, cerebral trauma, cerebral insufficiency and cerebral dementia and improve the cell oxygen content and microcirculation. According to a preparation method of the injection, the quality stability and safety of a final product can be fully guaranteed, a medicinal effect of the preparation is guaranteed, and side effects of the conventional preparation are eliminated.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a deproteinized calf blood extract and its injection and preparation method. technical background [0002] Calf blood deproteinized extract water injection, formerly known as: blood activity element injection, is made from fresh calf blood or serum through deproteinization, concentration, ultrafiltration or dialysis, and contains inorganic substances and small molecular organic substances sterile aqueous solution. It can enhance the uptake and utilization of oxygen and glucose by tissue cells, improve the hypoxic state of cells and the internal environment of the body, increase the blood flow of the heart, brain, liver and other organs, and improve microcirculation. It is used for the treatment of brain cell metabolic disorders such as cerebral ischemia, brain dementia, traumatic brain injury and brain insufficiency. At present, some preparation processes of calf blood prote...

AI Technical Summary

Problems solved by technology

These methods all have the problem of incomplete removal of protein. During clinical use, adverse reactions will occur due to the ineffective removal of miscellaneous proteins. Organic substances such as amino acids, small peptides, nucleotides, purines, etc. are polymerized into macromolecular substances. It is easy to cause allergic reactions; and when removing impurity proteins by enzymatic hydrolysis, high temperature, acid-base and other methods, there are disadvantages of destroying the original biological activity and reducing its medicinal value; in addition, the removal of impurity proteins by acid-base methods will also introduce foreign substances Source ions, which will reduce its medicinal value; in addition, due to poor process reproducibility, it is difficult to produce on a large scale

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