35 results about "Cellular oxygen" patented technology

The invention relates to the technical field of biological medicines, and particularly discloses a nanocapsule SMCPM and application thereof in medicines for treating or preventing osteosarcoma and pulmonary metastasis. The SMCPM is composed of a photosensitizer Ce6 carried by mesoporous silicon oxide-manganese oxide particles, an NRF2 inhibitor ML385 and an OS targeting peptide PT-7. Experiments prove that the SMCPM is high in penetrating power and strong in cell killing effect; by down-regulating NRF2 expression and reversing nuclear translocation, a biochemical inhibitory microenvironment can be effectively repaired, the tolerance of cells to active oxygen and epithelial-mesenchymal transition activated and induced by a NOTCH1 signal axis are remarkably reduced, the oxidative stress level of the cells is enhanced, and PIT-induced immunogenic cell death is promoted; the released Mn < 2 + > and double-stranded DNA can regulate and control an immunosuppressive microenvironment by activating an STING pathway in cells, thereby inhibiting the growth of osteosarcoma and preventing lung metastasis.

This invention discloses a traditional Chinese medicine prebiotic composition for treating high-altitude hypoxia and fatigue, and its preparation method. It belongs to the field of traditional Chinese medicine technology. The prebiotic composition comprises the following raw medicinal materials in parts by weight: 2-20 parts of Polygonatum odoratum, 2-20 parts of chicory, 2-20 parts of wolfberry, 3-30 parts of Codonopsis pilosula, 4-40 parts of Astragalus membranaceus, 2-20 parts of Angelica sinensis, 2-20 parts of jujube, and 2-20 parts of turmeric. The composition of this invention can improve the body's aerobic metabolism, enhance cellular oxygen utilization, strengthen high-altitude adaptability, and improve work capacity under hypoxic conditions. Furthermore, this composition helps relieve central and musculoskeletal fatigue, while having no toxic side effects, and exhibits significant anti-fatigue, hypoxia tolerance, and immune-regulating effects.

The invention discloses an application of an SIRT3 enriched exosome in preparation of a medicine for improving myocardial ischemia reperfusion injury. The preparation method comprises the following steps: screening to obtain polypeptide molecular gel G12 targeting HSP60, forming recombinant protein SIRT3-G12 from the G12 and SIRT3, constructing recombinant plasmids for expressing the recombinant protein, transfecting cells for co-expression, and extracting exosomes, thereby obtaining the SIRT3-enriched exosome SIRT3-Exo. In a cell experiment, the SIRT3-Exo has the effects of improving the mitochondrial metabolism function and reducing the oxidative stress and apoptosis level of the cells on hypoxia reperfusion myocardial cells. In an animal experiment, SIRT3-Exo is injected into a myocardial ischemia area of an I / R rat, so that the cardiac function is remarkably improved, the oxidative stress and inflammatory injury level is reduced, and angiogenesis is promoted. The result shows that the SIRT3-Exo has an obvious improvement effect on the myocardial ischemia reperfusion injury.

The application discloses application of cell endogenous metabolites in preparation of medicines for treating and / or preventing aging. The cell endogenous metabolites are any one or combination of phosphocreatine, hypotaurine and / or phosphoethanolamine. The application finds that phosphocreatine, hypotaurine and phosphoethanolamine can effectively restore cell growth, reduce cell SA-beta-Gal staining intensity, improve cell mitochondrial function and increase ATP yield, restore cell mitochondrial membrane potential, alleviate damage caused by cell oxidative stress and premature aging, and also prolong the life of nematodes and restore exercise capacity in the middle of aging. Meanwhile, the three metabolites have a synergistic effect, and by adjusting the matching concentration of the three metabolites, the same cell protection effect can be achieved by mixed administration. Since the endogenous metabolites are accurate, efficient and safe, the three cell endogenous metabolites provided by the application can be used for preparing anti-aging medicines.

The invention belongs to the technical field of fungal polysaccharide application, and particularly relates to a yellow-rose yellow-meat boletus polysaccharide as well as a preparation method and application thereof. The preparation method comprises the following steps: extracting crude polysaccharide from boletus rosea sporocarp through a water extraction and alcohol precipitation method, and then carrying out deproteinization, decoloration and DEAE-52 cellulose column chromatography separation and purification to obtain the purified polysaccharide BPP-1 of the boletus rosea water component. CCl4 is selected as an inducer of oxidative stress injury of the MIHA cells to construct a hepatic cell oxidative injury model, and the model is utilized to evaluate the protective effect of BPP-1 on CCl4-induced MIHA cell hepatic injury. The result shows that the BPP-1 reduces the release of ALT and AST, improves the GSH level and the SOD enzyme activity at the same time, inhibits the generation of a lipid peroxidation product MDA, and effectively reduces the damage of CCl4 to liver cells. The results show that BPP-1 has a certain protection effect on CCl4-induced hepatocyte injury, and BPP-1 may be a potential antioxidant and can alleviate oxidative injury of liver.

The present disclosure provides the use of catalase or a variant thereof or a nucleic acid sequence or a carrier or a delivery system in the preparation of a pharmaceutical composition or a cosmetic composition for scavenging reactive oxygen species (ROS), alleviating oxidative stress.

Peptide compounds and their use in the treatment of diseases and disorders caused by or characterized by cellular oxidative stress. The disclosed compounds comprise or consist of an amino acid sequence having the formula Gly-X-Thr-Pro, where X is a sequence of 3 amino acids selected from Arg-Ala-Chs(acid). Further, the disclosed compounds comprise or consist of the amino acid sequence Gly-ArgGly-Cys(acid)-Gly-Gly-Gly-Asp-Gly.

The application discloses a two-photon fluorescent probe and uses the same for tumor microenvironment monitoring. The application opens the two-photon fluorescent characteristics of the material by responding to the overexpression of SO2 and H2O2 at the tumor site in stages, monitors and indicates the changes of cell oxidative stress and homeostasis balance, and is further used for early diagnosis of diseases caused by internal environment homeostasis imbalance. The application regulates the cancer cell uptake capacity of the molecule by introducing suitable lipophilic and hydrophilic groups, increases the recognition site of biological thiols by introducing conjugated olefin bonds, and further realizes the step-by-step recognition of endogenous signal molecules by using the easiness of substitution reaction, enhances the fidelity of the signal, and is expected to realize the in-situ activated two-photon fluorescence, early and accurate diagnosis of a series of diseases such as cardiovascular diseases, Alzheimer's disease and the like induced by internal environment homeostasis imbalance.

The invention belongs to the technical field of biological medicine, and particularly relates to superoxide dismutase HSTM-SOD and application thereof. The amino acid sequence of the superoxide dismutase HSTM-SOD provided by the invention is selected from at least one of sequences as shown in SEQ ID NO: 1 to SEQ ID NO: 8, or a sequence with more than 80% of homology with the amino acid sequence. The HSTM-SOD with different sequences, which is provided by the invention, generally has excellent thermal stability, pH stability, good compatibility and super-strong membrane crossing capability; the HSTM-SOD shows remarkable capabilities in the aspects of promoting cell sunburn repair, promoting cell oxidative damage repair, resisting ferroptosis caused by TalaA, promoting wound healing of diabetic patients, improving age-related macular degeneration and the like, and has a wide application prospect.

The invention belongs to the technical field of biological medicine, and particularly relates to application of pseudo-ginseng carbon quantum dots in preparation of a medicine for treating renal ischemia-reperfusion injury, the carbon quantum dots are synthesized by taking pseudo-ginseng as a raw material through a hydrothermal method, and the pseudo-ginseng carbon quantum dots have the antioxidant enzyme activity property, can effectively relieve cell oxidative stress and inhibit secretion of proinflammatory cytokines, and can be used for preparing a medicine for treating renal ischemia-reperfusion injury. The pseudo-ginseng carbon quantum dots specifically gather in the damaged kidney, so that the renal ischemia reperfusion injury of the mouse is obviously relieved; the pseudo-ginseng carbon quantum dots can relieve kidney injury through the following ways: reducing abundance of escherichia coli-shigella, reducing the level of indol sulfate in the kidney, regulating tryptophan-indole metabolism, regulating an AHR-Raf-ERK signal channel, down-regulating the levels of TNF-alpha and malonaldehyde, and regulating the level of glutathione. A catalytic mechanism and a multi-omics regulatory pathway of the pseudo-ginseng carbon quantum dots in renal ischemia reperfusion injury are disclosed, and experimental evidence is provided for'intestinal tract-kidney axis' in kidney disease treatment.

The present application belongs to the technical field of bioengineering, and particularly relates to a tuna fish bone antioxidant oligopeptide and a preparation method thereof.The tuna fish bone antioxidant oligopeptide has good scavenging activity, and has good thermal stability and digestion resistance; the mechanism of action is to promote the release of nitric oxide (NO), improve the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and total antioxidant capacity (T-AOC), and reduce the levels of malondialdehyde (MDA) and lactate dehydrogenase (LDH); JQYG-1 can protect HUVEC cells from oxidative damage induced by hydrogen peroxide; JQYG-1 can exert an antioxidant effect and protect vascular endothelial cells, and can provide a candidate drug for the prevention and treatment of cardiovascular and cerebrovascular diseases such as hypertension, coronary heart disease, cerebral thrombosis, myocardial infarction, atherosclerosis and heart failure.

The application provides application of pig's tooth saponin volatile oil in preparation of a medicine for treating cerebral ischemia-reperfusion injury, and a preparation method of the pig's tooth saponin volatile oil is as follows: pig's tooth saponin decoction pieces are taken, water and n-hexane are added, and volatile oil is obtained through steam distillation; the volatile oil is collected, anhydrous sulfuric acid is added, and water is removed; and the mixture is left overnight to obtain the pig's tooth saponin volatile oil. The prepared pig's tooth saponin volatile oil acts on key target points of VEGFA, SRC, MAPK8, PIK3CA and TNF through active ingredients of paeonol, linalool, methyl eugenol, anethole and eugenol, regulates multiple signal pathways of sphingolipids, TNF and VEGF, inhibits cell oxidative stress injury, inhibits release of inflammatory factors and regulates nerve function, so as to play a role in treating cerebral ischemia-reperfusion injury, and lay a foundation for further in-depth study on a treatment mechanism of pig's tooth saponin in treating 'opening the orifices and connecting the heart and the lung'.

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

The invention provides a preparation method of 5-methyl kaempferol carbon quantum dots. The method has the advantages that a green synthesis process is adopted, the safety is high, and the biocompatibility is good; the carbon quantum dots of the 5-methyl kaempferol prepared by the invention are used as an efficient ROS (reactive oxygen species) scavenger, have obvious antioxidant and anti-inflammatory effects, can obviously reduce oxidative injury of pulmonary epithelial cells, and can effectively promote lung injury repair of acute lung injury; compared with common kaempferol, the prepared carbon quantum dot of 5-methyl kaempferol has higher anti-inflammatory activity and better intracellular delivery efficiency, and has a better repairing effect on acute lung injury.

The invention relates to a ventricular assist device integrated with lactic acid detection and a flow control method thereof.The ventricular assist device comprises a lactic acid sensor which comprises a signal conversion module and an electrode in direct contact with blood, and the electrode is fixed to the inner wall of a blood flow channel of the ventricular assist device; the output end of the controller generates a rotating speed adjusting instruction; and the magnetic suspension pump changes the pump rotating speed according to the rotating speed adjusting instruction. Monitoring faults between physical indexes such as blood pressure and flow and cell oxygen utilization can be solved; the problem that complication development cannot be recognized in time due to time delay in traditional lactic acid detection is solved; a doctor is helped to accurately judge the condition of a patient, accurately find the time point of using or evacuating the ventricular auxiliary equipment and optimize use parameters; doctors are helped to accurately judge patient conditions and evaluate the use effect of the ventricular assist equipment. Compared with the prior art, lactic acid detection can be carried out on a patient in the ventricular assist process, and the lactic acid change can be rapidly judged.

The invention belongs to the technical field of chemistry, and particularly relates to an artificial synthesis method and application of a compound theanine diglucoside TFG. Theanine-fructosyl-glucose (TFG) is a Maillard reaction product, has obvious antioxidant activity, and is not systematically studied at present, so that the antioxidant activity of the theanine-fructosyl-glucose (TFG) can be obviously improved, and the antioxidant activity of the theanine-fructosyl-glucose (TFG) can be obviously improved. The invention provides an efficient artificial synthesis method of the compound TFG, the synthesis cost is reduced, a material basis is provided for subsequent research, and the method has the characteristics of economy, high efficiency, simplicity in operation, safety and the like, and is suitable for industrial production. Subsequently, activity screening of HT22 mouse hippocampal neuronal cells and HUVEC human vascular endothelial cells in vitro proves that the compound TFG can remarkably relieve cell oxidative damage induced by hydrogen peroxide (H2O2). Based on the synergistic protection effect of the TFG on neurons and vascular endothelial systems, the TFG is suitable for developing antioxidant drugs for treating Alzheimer's disease.

The present invention concerns an aqueous solution comprising molecular hydrogen and at least one salt, wherein said solution has an ionic strength in the range from 0.001 mol / l to 1 mol / l and comprises ascorbic acid or a salt thereof. The solution can be used for ameliorating the activation of cell mechanisms that reduce cellular oxidative stress and cell toxicity by transdermal administration to a subject and for use in treating oxidative stress-related diseases.

The invention discloses an octapeptide with antioxidant activity. The octapeptide is characterized in that the sequence of the octapeptide is amino terminal-SPDIFNPQ-carboxyl terminal. The antioxidant activity of the peptide is evaluated through the protective effect of the peptide on oxidative stress injury of HepG2 cells. Results show that the peptide can effectively relieve H2O2-induced cell oxidative stress injury, and can be further developed into a functional food additive or a medicine for relieving and treating oxidative stress related diseases.

The subject invention relates to a novel compound and method for treating cancer in a subject using photodynamic therapy (PDT). The novel compound comprises zinc(II) phthalocyanine substituted with two or four nitric oxide (NO)-releasing moieties that release NO upon interaction with the intracellular glutathione, which reduces the cellular oxygen consumption rate, thus relieving the hypoxic status of cancer cells. Upon light irradiation, the novel compound generates reactive oxygen species (ROS), induces cytotoxicity under a hypoxic condition, overcoming the oxygen-dependent nature of PDT, and elicits the release of damage-associated molecular patterns, inducing the maturation of dendritic cells and triggering an antitumor immune response.

The application discloses a nano drug based on nitrogen-doped selenized carbon dots and a preparation method and application thereof, and belongs to the technical field of biological medicines, wherein the preparation method of the nano drug comprises the following steps: placing an amino acid compound and a small molecule drug in a solvent to mix, adjusting the pH value to be alkaline, then performing a heating reaction to obtain a reaction solution; the amino acid compound is one or more of L-selenocystine, L-selenocysteine and L-selenomethionine; the small molecule drug is metformin hydrochloride or phenformin; and the nano drug is obtained by separating and purifying from the reaction solution. The nano drug can significantly relieve cell oxidative damage induced by hydrogen peroxide, can effectively remove ROS excessively accumulated in a damage site, and can inhibit the progress of secondary damage. In addition, the nano drug can regulate four key signal paths of antioxidation, anti-inflammation, anti-apoptosis and axon regeneration and myelination, and can synergistically promote the nerve repair process after spinal cord injury.

The application provides a near-infrared response magnesium-releasing and antioxidant double-layer microneedle and a preparation method and application thereof. The double-layer microneedle comprises a needle tip layer and a backing layer, the needle tip layer contains MoS2@PDA@Mg 2+ The application discloses a composite particle, which is composed of molybdenum disulfide nanosheets, surface-coated polydopamine and coordination-loaded magnesium ions. The preparation method comprises the following steps: one-step reaction of molybdenum disulfide, dopamine and magnesium salt to obtain the composite particle, and then preparation of the composite particle into a needle tip layer precursor solution together with GelMA, PVA and the like, and two-step light cross-linking molding with a backing layer precursor solution. The double-layer microneedle can rapidly release magnesium ions under near-infrared irradiation, the release amount is 4.6 times that of a non-irradiation group, can significantly scavenge DPPH, ABTS and other free radicals, can relieve cell oxidative stress induced by lipopolysaccharide, and can effectively promote new bone formation and trabecular reconstruction in a rat tooth socket model. The application realizes the synergistic effect of light-controlled magnesium release and antioxidant, and is suitable for the fields of neural microenvironment reconstruction and bone regeneration.

The invention provides a preparation method of carnosic acid carbon quantum dots. The method has the advantages that a green synthesis process is adopted, the safety is high, and the biocompatibility is good; the carbon quantum dots of the carnosic acid carbon quantum dots prepared by the preparation method disclosed by the invention are used as an efficient ROS scavenger, have remarkable antioxidant and anti-inflammatory effects, can remarkably reduce oxidative injury of pulmonary epithelial cells, and can effectively promote lung injury repair of acute lung injury; compared with carnosic acid, the carnosic acid carbon quantum dot prepared by the invention has higher anti-inflammatory activity and better intracellular delivery efficiency, and has a better repair effect on acute lung injury.

Peptide compounds and their use in treating diseases and disorders that cause, are caused by, or are characterized by cellular oxidative stress.

This invention belongs to the field of biomedical technology and discloses the application of the small molecule tripeptide Ser-Ser-Cys (SSC) in inhibiting the phenotypic transformation of vascular smooth muscle cells (VSMCs). SSC is an endogenous differentially expressed tripeptide screened and identified from the serum of hypertensive non-atherosclerotic patients using non-targeted metabolomics technology. In vitro experiments show that SSC can effectively inhibit angiotensin II (Ang II)-induced abnormal proliferation, migration, and phenotypic transformation from contractile to synthetic VSMCs. Its mechanism of action is related to the regulation of the Keap1 / Nrf2 signaling pathway, improvement of cellular oxidative stress, and mitochondrial function. This invention provides a new approach for using SSC in the preparation of drugs or functional foods for the prevention and treatment of hypertension-related vascular remodeling.

The present invention belongs to the technical field of daily chemical products, and relates to an anti-aging composition for targeting and activating cell energy, a preparation method thereof and an application. The composition includes β-nicotinamide mononucleotide, Paeonia lactiflora root extract, Bacillus fermentation product, and Caesalpinia spinosa gum. The composition product of the present invention aims at the energy shortage caused by skin mitochondrial damage, combs and improves mitochondrial dysfunction, activates mitophagy, increases the expression of silent information regulator 3 (SIRT3) in mitochondria, activates cellular oxygen consumption, protects mitochondria from oxidative stress, increases intracellular energy reserve (ATP), rationally combines various components, promotes cell metabolism in multiple dimensions, accelerates skin renewal, realizes targeted anti-aging of the skin, and with the sustained-release effect brought by Caesalpinia spinosa gum, ensures the immediate and long-term anti-aging activity of the composition, improves bioavailability, is mild and non-irritating to the skin, and can be widely applied in the cosmetics industry.

The invention provides application of a PPP1R15A gene in regulation and control of oxidative stress of cells, and belongs to the technical field of gene engineering. Experiments show that oxidative stress of bovine mammary epithelial cells can be inhibited by inhibiting expression of the PPP1R15A gene. The invention also provides an application of the siRNA for knocking out or inhibiting expression of the PPP1R15A gene in preparation of a medicine for inhibiting oxidative stress of cells, wherein the sequence of the siRNA is shown as SEQ ID NO.11 and SEQ ID NO.12. The invention also provides an application of the siRNA for knocking out or inhibiting expression of the PPP1R15A gene in preparation of a medicine for inhibiting oxidative stress of the cells. The siRNA provides a new direction for searching a novel anti-oxidative stress treatment strategy.

Peptide compounds and their use in treating diseases and disorders that cause, are caused by, or are characterized by cellular oxidative stress.

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

The invention relates to the technical field of pharmaceutical preparations, and discloses a composite oral soluble film for dispelling effects of alcohol and protecting liver and a preparation method thereof. The preparation method comprises the following steps: providing a nanofiber reservoir solution containing silk fibroin, a deep-eutectic solvent and polyethylene glycol; providing a backing layer solution containing silk fibroin and polyethylene glycol; carrying out electrostatic spinning by adopting the nanofiber reservoir solution to obtain a nanofiber reservoir; carrying out electrostatic spinning by adopting the backing layer solution, and forming a backing layer on the nanofiber reservoir, so as to obtain a composite oral soluble film; wherein the deep eutectic solvent contains the dihydromyricetin. The composite oral soluble film prepared by adopting the preparation method has good biocompatibility and biodegradability, can enable drugs to be released and diffused to mucosa in one direction and enter the liver through blood circulation to accelerate alcohol metabolism and inhibit oxidative damage of hepatocytes, and has wide application prospects.

The invention discloses an oleic acid-crocetin compound preparation and application thereof, oleic acid and crocetin are adopted to form a mixed system to act on PC12 cells damaged by H2O2 induced oxidation, the survival rate of the PC12 cells can be obviously improved compared with the single use of crocetin with the same concentration, and the obvious effect does not exist after linoleic acid and linolenic acid are mixed with crocetin. The invention reveals that oleic acid can enhance the cell oxidative damage resistance of crocetin, provides a brand new scheme for research and development of reagents or compositions for resisting cell oxidative damage, and has important biomedical application value.