47 results about "Cellular oxygen" patented technology

A probe for sensing and imaging intracellular oxygen comprises an oxygen-sensitive fluorescent or phosphorescent dye combined with a hydrophilic macromolecular carrier and a cell loading agent. A method for sensing cellular oxygen using the probe is also described.

The present invention encompasses the use of compounds for a novel approach to treat and prevent diseases, conditions, and disorders such as diabetes and ischemic reperfusion injury. Compounds of the invention, including but not limited to BAM15((2-fluorophenyl){6-[(2-fluorophenyl)amino](1,2,5-oxadiazolo[3,4-e]pyrazin-5-yl)}amine), a mitochondrial uncoupler, can improve glucose tolerance, increases cellular oxygen consumption, treat or prevent kidney ischemia reperfusion injury reverse insulin resistance, reverse or treat hyperinsulinemia, and reverse or treat hyperlipidemia. The present invention further provides novel compounds as well as methods for identifying compounds with the same or similar properties as BAM15.

The invention discloses a cellular oxygen carrier chemical-looping reforming reactor. The cellular oxygen carrier chemical-looping reforming reactor comprises a reactor main body which comprises an outer wall and an outer heating barrel, wherein cellular oxygen carriers are formed in the outer wall and the outer heating barrel and are jointed with the outer wall and the inner wall of the outer heating barrel, and cellular pore paths parallel to the outer wall and a main shaft of the outer heating barrel are arranged in the cellular oxygen carrier and are pairwise spaced; and the tops of the outer wall and the outer heating barrel are connected with a top gas collecting region through upper flanges, the bottoms of the outer wall and the outer heating barrel are connected with a bottom gas storage region through lower flanges, a tail gas outlet is connected outside the top gas collecting region, a tee-junction gas inlet is connected outside the bottom gas storage region, and the bottom gas storage region is internally provided with a radiation type gas pre-distribution device connected with the tee-junction gas inlet. The cellular oxygen carriers have the advantages of having heat storage function, being capable of helping the reactor to maintain reaction temperature, alleviating the thermal load of external heating equipment and being beneficial to the stable proceeding of reaction.

The invention belongs to the technical field of molecular biology, and relates to a method for screening traditional Chinese medicine monomers, specifically relating to a method for screening monomer ingredients which have protective effect for oxidative damages to liver cells from Chinese traditional medicines by using mitochondrion index technique. In the invention, mitochondrial function detection is used as the core technology and methods of cell proliferation detection, mitochondrial reactive oxygen species generation detection, mitochondrial membrane potential detection, energy substance ATP in mitochondria detection and mitochondrial cytochrome C detection are adopted. Mitochondrial function changes are detected after H7702 cells, which are subject to oxidative damages induced by H2O2, are treated by the traditional Chinese medicine monomers of SalB, protocatechualdehyde, sodium danshensu, cordyceps polysaccharose, cordycepin and cordycepic acid, and the traditional Chinese medicine monomers, which have protective effect for oxidative damages to liver cells, are screened. The invention further provides applications of the traditional Chinese medicine monomers screened in preparing drugs for preventing and treating liver diseases such as fatty liver, viral hepatic diseases and liver fibrosis.

The invention provides application of vaccarin for resisting oxidation and high-glucose damage, and belongs to the technical field of traditional Chinese medicament application. The application first discovers that vaccarin has the activity of protecting oxidative stress injury and high-glucose damage of endothelial cells and can be used for treating a series of diseases induced by atherosclerosis, cataract, aged-related macular degeneration and other endogenous or exogenous active oxygen invasions, and preventing and treating diabetes mellitus, hypertension and related diseases caused by high glucose. The vaccarin can be applied to medicinal preparations, nourishment, healthy products, cosmetics and the like.

The invention relates to the technical field of cosmetics, in particular to an antioxidative composition and a preparation method and application thereof. In the composition, fructus schizandrae and soybeans are compounded, an experiment shows that the composition has a remarkable clearing effect to DPPH free radicals, cell oxidation damage caused by t-Bhp stimulation can be reduced remarkably, growth of cells formed by keratoderma is protected effectively, and an obvious synergistic antioxidative effect is achieved.

The invention discloses aesculetin with anti-oxidative and anti-aging activity as well as a preparation method and an application of aesculetin. The preparation method comprises preparation steps as follows: (1), cortex fraxini residues are dried, sterilized for 30 min under ultraviolet irradiation and taken out; (2), distilled water which is 8 times the sterilized cortex fraxini residues in parts by weight is added to the sterilized cortex fraxini residues for fermentation, after fermentation, a sample is taken out, dried, smashed and screened by a 30-mesh sieve, and fermented cortex fraxini residue powder is obtained; (3), an organic extracting agent is added to the fermented cortex fraxini residue powder for reflux extraction, extracting solutions are combined, a combined solution is filtered, and the organic extracting agent is subjected to reduced-pressure recovering and concentrated into thick paste; (4), distilled water is added to the thick paste for dilution, heating dissolution is performed, an obtained solution is filtered while still hot, and a filtrate is separated, purified and decolorized and then is subjected to reduced-pressure concentration and freeze drying finally. Aesculetin has a function of eliminating oxygen free radicals, has a protecting effect on cell DNA oxidative damage caused by lipid peroxide and can effectively delay skin cell senescence.

A microplate apparatus having a bottom plate defining a plurality of very small microwells (for example, each having a volume of 0.02 or less) for containing sample material and a top plate defining a plurality of domes, each of which housing a sensors for measuring biological parameters, such as cellular oxygen consumption, pH changes, calcium uptake, and metabolic reduction of fluorescent dyes. The top and bottom plates may be composed of quartz. The microplate assembly may be fabricated using a method involving applying an etching solution to each of the top and bottom plates to create the domes and microwells. Additionally, etching solution may be applied to the bottom plate to create a raised lip region around each of the microwells. The etched-away areas surrounding the lip regions facilitate the changing of microwell solution without disrupting the samples.

The invention provides a method for applying L-selenomethionine to animal cell antioxidation and a product thereof, which relate to the technical field of animal cell culture. The method comprises thefollowing step of adding an L-selenomethionine working solution into a culture system of animal cells so that the final concentration of L-selenomethionine in the culture system of animal cells is 0.5-1.5 mu M; wherein the concentration of the L-selenomethionine working solution is 5-15 [mu] M. The application method of L-selenomethionine in animal cell antioxidation alleviates the lack of an animal cell antioxidation method which can effectively reduce cell oxidative damage and maintain cell activity, and is low in cytotoxicity, simple and convenient to operate and low in cost in the prior art. The invention also provides an animal cell antioxidant containing the L-selenomethionine, an animal cell culture medium containing the L-selenomethionine or the animal cell antioxidant containingthe L-selenomethionine, and a medicine or a feed additive containing the L-selenomethionine or the animal cell antioxidant containing the L-selenomethionine.

The invention discloses a porcine epithelial cell oxidative stress model, an establishment method therefor and application of the porcine epithelial cell oxidative stress model. The establishment method for the porcine epithelial cell oxidative stress model comprises the following steps: inducing porcine epithelial cells by AAPH to continuously generate stable endogenous free radicals, detecting the oxidative damage degree of the cells, and determining a stress model of oxidative damage. According to the porcine epithelial cell oxidative stress model, the optimal concentration for constructingthe porcine small intestinal epithelial cell oxidative stress model induced by the AAPH is determined by a series of tests, and the oxidative damage is evaluated by the established oxidative stress model, so that the convenient and stable intestinal epithelial cell model is provided for further research of the influence of the oxidative stress on animal intestinal health, and the model has important significance for maintaining human health and reducing direct losses and indirect losses caused by oxidative stress in animal husbandry production.

Provided is a cell culture device that has a simple structure and ensures sufficient nutrient supply to cells and oxygenation of the cells to thereby enable mass cell culture. The cell culture device comprises: a cell culture container which is an approximately cylindrical body provided with a flat bottom part at the lower end; a dish-shaped body having an approximately disc shape which is provided with a plurality of magnetic attraction members, said magnetic attraction members being positioned in the circumferential part at equal intervals, and horizontally disposed in a non-contact state within a hollow space inside the cell culture container; and a cyclic body which is provided with a plurality of magnetic attraction members and positioned outside the cell culture container so that the cell culture container is located within the cycle thereof. The cyclic body and the dish-shaped body move vertically in magnetic conjunction to thereby agitate a medium and supply nutrients to cells.

The present invention relates to a method for preventing cancer by using a phthalide compound, wherein the phthalide compound has an effect of increasing the oxygen release efficiency of hemoglobin (Hb) of a subject to increase the oxygenation level of organs and tissue cells, thereby preventing the cellular oxygenation level against falling below the critical cellular oxygen requirements under which the normal cells can turn cancerous. Although cancer may be caused by a variety of reasons, including congenital inheritance, external environment, air pollution or poor living and dietary habits, there is only one primary and common reason in causing cancers, the excessively low cellular oxygenation level. When the oxygenation level of any cell falls below 60% of its physiological oxygen requirements, the normal cell may turn cancerous. The present invention is to use the phthalide compound to substitute for or cooperate with 2,3-BPG of a subject to lower the oxygen affinity of hemoglobin (Hb) and to facilitate the release of oxygen from hemoglobin to tissue cells, as a result, the cellular oxygenation level is maintained always higher than the critical threshold for the normal cells to turn cancerous, and by doing so to prevent the normal cells from turning cancerous and prevent the developments of cancers.

The invention provides application of an xenicane type diterpenoid compound epoxyhydroxyacetyldictyolal to preparation of antioxidative neuroprotective drugs. The diterpenoid compound epoxyhydroxyacetyldictyolal can significantly inhibit the neuronal PC12 cell oxidative damage induced by H2O2, promote PC12 cell colony formation, and inhibit release of lactate dehydrogenase (LDH). Diterpene is an excellent antioxidant, has the protective effect on the oxidative damage of nerve cells, and can be used for preventing or treating the cerebral ischemia-reperfusion injury.