Dielectrophoresis-based chip for separation of deformable microparticles

Abstract

The invention discloses a dielectrophoresis-based micro-fluidic chip for separation of deformable microparticles. The dielectrophoresis-based micro-fluidic chip is formed by connection of a primary pipeline and a secondary pipeline, wherein an electric field is arranged in the primary pipeline and can drive deformable microparticles to move towards the secondary pipeline, and the secondary pipeline is divided into an upper pipeline and a lower pipeline and used as a separation pipeline for the microparticles after movement. To-be-separated microparticles are allowed to move towards secondary pipelines located at different directions after passing through a contraction structure by setting different parameters like electric field intensity and the radius of a contraction opening. The dielectrophoresis-based micro-fluidic chip provided by the invention has the advantages that the deformable microparticles may be biological cells, and the biological properties and activity of the biological cells are not damaged in the process of separation; through setting of different parameters, particles with radiuses in a certain range can be perfectly separated; a separation structure is simple; separation process is good in operability; a separation channel is short; separation time is short; and separation efficiency is high.

Description

technical field [0001] The invention relates to a microfluidic chip for separating deformable particles based on dielectrophoresis. Background technique [0002] The separation of microparticles is an important method for product purification. This technology can not only obtain relatively high-purity particulate matter, but also guarantees various subsequent tests; with the development of science and technology, the separation technology of microparticles is in the It has a wide range of applications in biological research, detection and analysis, environmental science, nanotechnology and medicine. [0003] At the micro-scale, the separation of particles involves a variety of interdisciplinary fields, and is a relatively cutting-edge scientific research, which involves multiple disciplines such as fluid mechanics, micro-processing and micro-manufacturing; Inexpensive, controllable fluid, less reagent consumption, high precision, etc., to separate and study particles at the...

AI Technical Summary

Problems solved by technology

[0005] There are several common methods used in the field of microparticle separation technology, such as chemical control methods, mechanical control methods, laser methods, magnetic control methods, electric field control methods, etc.; among them, chemical control methods are often used in practical applications. It is destructive to the sample particles, and the experiment is irreversible; the magnetic control method needs to add magnetic materials under certain requirements before the test, which is also destructive to the particles, and the experimental steps are also cumbersome. The final experimental results have certain limitations

Images