Method for extracting total flavone from folium ginkgo with ultrasound wave assisted

Abstract

The invention relates to a new traditional Chinese medicine effective components extraction technique, in particular to a new auxiliary method to effectively extract total flavonoids from ginkgo biloba via ultrasonic. The invention adopts orthogonal experiment design scheme and is based on technical parameters like ultrasonic occurring mode in screening extraction process, ultrahigh ultrasonic intensity, rapid action time and mode, solvents, system temperature and stirring method, etc, to prepare fresh ginkgo leaf tablets; the tablets are baked for 24 hours under conditions of 60 degrees centigrade, sieved (0.45 Mu m) to produce raw material; the raw material is put in ultrasonic extraction pot and is added with alcohol with 60 percent alcohol, with a ratio of material to liquid (Kg:L)1:15 to 17, and is mixed. The ultrasonic occurring mode is: separately stricken ultrasonic sound generating mechanism, radial vibration columnar transducer; the ultrasonic power is: 100W; the ultrasonic frequency is 33kHz;the distilling way is: ultrasonic function every two minutes for 3 minutes, with the total extraction time 25 minutes; the extraction temperature is 55 degrees centigrade; and the stirring mode is air-lift stirring. Prepared in the above method, the extraction rate of the ginkgo biloba total flavonoides is as high as 97.35 percent.

Description

1. Technical field [0001] The invention relates to a new technology for extracting effective components of traditional Chinese medicines, in particular to a new method for efficiently extracting total flavonoids from ginkgo leaves with the aid of ultrasonic waves. 2. Background technology [0002] The chemical meaning of extraction is to use a suitable solvent and an appropriate method to leach the required components from the sample material as completely as possible, avoiding or reducing the introduction of harmful components or other impurities. Extracting chemical components from plants for medicinal purposes has a history of nearly 5,000 years in the traditional medicine of the motherland. Traditional extraction methods can be divided into two categories according to the different energy requirements of the extraction process. One is the heating extraction method, which mainly includes different methods such as decoction extraction, reflux extraction and Soxhlet extrac...

AI Technical Summary

Problems solved by technology

However, there are some defects in the application of these types of extraction processes.

Specifically, the disadvantages of the chemical solvent extraction method have energy, large consumption of raw materials, low extraction efficiency, and large industrial pollution discharges (for example, described in the CN1129218A method)

Although the supercritical fluid extraction method has been improved on the extraction efficiency, the equipment required by this type of method is complicated, the supercritical solvent selection range is narrow, the product stability is poor, and it is unfavorable for large-scale production etc. (for example, described in CN1172669A method)

Microwave extraction overcomes some defects in the above two methods, but the thermal effect of microwave in the extraction process will lead to the degradation of some heat-sensitive components in the extract, which seriously affects the biological activity of the extract, and also limits the wide application of this method ( For example, CN1281856A)