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Method for extracting Indian epimeredi herb dilactone from Indian epimeredi herb leaves

A technology for parsnip and dilactone is applied in the field of separation and preparation of parsnip dilactone, which can solve the problems such as no patent report on the preparation method of high-content parsnip dilactone, and achieves short preparation period, short time-consuming, and easy preparation. large amount of effect

Inactive Publication Date: 2013-12-18
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no patent reports related to the preparation method of high-content parsnip dilactone

Method used

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  • Method for extracting Indian epimeredi herb dilactone from Indian epimeredi herb leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Take dry parsnip leaves, crush them, control the particle size to 40 mesh, weigh 200g, add 15 times of 80% ethanol solution, and extract by ultra-high pressure method, the extraction pressure is 400MPa, the temperature is 35°C, and the holding time is 5min , repeat boosting and depressurization twice, combine the extracts, concentrate under reduced pressure, and perform chromatographic column chromatography. The chromatographic column uses neutral magnesium oxide as the filler, the particle size is 100 mesh, the column pressure is 5bar, and the column diameter-to-height ratio is 1 :10, petroleum ether-ethyl acetate 12:1, 5:1, 1:1 gradient elution, collect the target eluent, concentrate and dry to obtain the crude extract; use high-speed countercurrent chromatography to separate and purify the windproof in the crude extract Oxydilide, with a volume ratio of 5:7:5:3 n-hexane-ethyl acetate-methanol-water as a two-phase solvent system, the upper phase is filled with a high-s...

Embodiment 2

[0028] Take dry parsnip leaves, crush them, control the particle size to 40 mesh, weigh 500g, add 12 times of 90% ethanol solution, and use the ultra-high pressure method to extract, the extraction pressure is 300MPa, the temperature is 45°C, and the holding time is 10min , repeat boosting and depressurization twice, combine the extracts, concentrate under reduced pressure, and perform chromatographic column chromatography. The chromatographic column is filled with neutral magnesium oxide, the particle size is 100 mesh, the column pressure is 6bar, and the column diameter-to-height ratio is 1 :10, petroleum ether-ethyl acetate 12:1, 5:1, 1:1 gradient elution, collect the target eluent, concentrate and dry to obtain the crude extract; use high-speed countercurrent chromatography to separate and purify the windproof in the crude extract Oxydilide, with a volume ratio of 5:7:5:3 n-hexane-ethyl acetate-methanol-water as a two-phase solvent system, the upper phase is filled with a h...

Embodiment 3

[0030] Take dried parsnip leaves, crush them, control the particle size to 40 mesh, weigh 500g, add 15 times of 90% ethanol solution, and extract by ultra-high pressure method, the extraction pressure is 400MPa, the temperature is 40°C, and the holding time is 8min , repeat boosting and depressurization 3 times, combine the extracts, concentrate under reduced pressure, and perform chromatographic column chromatography. The chromatographic column uses neutral magnesium oxide as the filler, the particle size is 100 mesh, the column pressure is 7.5bar, and the column diameter-to-height ratio is 1:12, petroleum ether-ethyl acetate 11:1, 4:1, 1:1 gradient elution, collect the target eluent, concentrate and dry to obtain the crude extract; use high-speed countercurrent chromatography to separate and purify the Parsnip dilactone, with a volume ratio of 5:7:5:3 n-hexane-ethyl acetate-methanol-water as a two-phase solvent system, the upper phase is filled with a high-speed countercurren...

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Abstract

The invention relates to a method for extracting Indian epimeredi herb dilactone from Indian epimeredi herb leaves, which comprises the following steps: 1) performing ultrahigh-pressure extraction on the Indian epimeredi herb leaves by use of an alcohol solution, combining the extract liquid and recovering a solvent to obtain an extract of the Indian epimeredi herb leaves; 2) performing magnesium oxide column chromatography on the extract, performing gradient elution by use of petroleum ether-ethyl acetate, collecting the target eluent and concentrating and drying to obtain a crude extract; 3) performing chromatographic separation on the crude product to obtain Indian epimeredi herb dilactone. The method for extracting Indian epimeredi herb dilactone, provided by the invention, has the advantage that abundant Indian epimeredi herb dilactone can be effectively extracted from the Indian epimeredi herb dilactone leaves so as to provide abundant Indian epimeredi herb dilactone for large-scale preparation of medicines taking Indian epimeredi herb dilactone as a main active ingredient.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of traditional Chinese medicines, and relates to a method for separating and preparing parsnip dilactone from leaves of parsnips. Background technique [0002] Ovatodiolide, the molecular formula is C20H24O4, the molecular weight is 328.41, and the structural formula is [0003] [0004] Parsnip dilactone is derived from the leaves of the Lamiaceae (Labiatae) parsnip Anisomeles indica (L.) O.Ktze. Parsnip dilactone has cytotoxic activity, and its IC50 on KB cells is 0.6 μg / mL; it has antihypertensive effect, and inhibits the contraction and Ca 2+ flow, causing a transient hypotensive effect in dogs, but not inhibiting angiotensin-converting enzyme (ACE). Inhibition of myocardial contraction, orange anti-calcium ion flow. [0005] At present, there are no patent reports related to the preparation method of high-content parsnip dilactone. Contents of the invention [0006]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/08
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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