Method for separating and purifying 5'-cytidylic acid and derivatives thereof

A technology for separation, purification and derivatives, which is applied to the preparation of sugar derivatives, sugar derivatives, and sugar derivatives, etc., can solve the problems of large equipment scale, long production cycle, long operation process, etc., and achieves high selectivity and simple steps. , does not destroy the effect of biological activity

Inactive Publication Date: 2010-02-17
上海新浦特种助剂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the method is theoretically feasible, there are many disadvantages, such as the operation process is too long, the equipment scale is large, the productio

Method used

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  • Method for separating and purifying 5'-cytidylic acid and derivatives thereof
  • Method for separating and purifying 5'-cytidylic acid and derivatives thereof
  • Method for separating and purifying 5'-cytidylic acid and derivatives thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Step 1, prepare the solution to be separated;

[0047] Take 1.5kg of 5'-cytidylic acid, 1.5kg of sodium chloride, 1kg of sodium dihydrogen phosphate, add 35kg of distilled water, adjust the pH value to 5.0±0.1 with 30% sodium hydroxide solution, and then add distilled water to a total weight of 38kg;

[0048] Step 2, pour the solution to be separated into the feed liquid tank;

[0049] Step 3, carry out desalination operation;

[0050] Step 3.1, operate the equipment, and adjust the pressure to 1.2-1.5 MPa by the pressure regulating device, so that the solution to be separated passes through the nanofiltration membrane;

[0051] Step 3.2, collect the permeate, and return the retained liquid to the feed liquid tank;

[0052] Step 3.3, control the material concentration and keep the liquid level of the material in the feed liquid dregs, that is, add 10 kg of distilled water when the feed liquid in the feed liquid dregs reduces the weight by 10 kg (i.e. the permeate is c...

Embodiment 2

[0064] Step 1, prepare the solution to be separated;

[0065] Take 15Kg of the water phase extracted and stratified in the crude synthesis production of 5'-cytidylic acid workshop, add 22Kg of distilled water to dilute, adjust the pH value to 5.0±0.1 with 30% sodium hydroxide solution, and the solution contains about 5' - 2.65kg of cytidylic acid, the inorganic salts are mainly sodium chloride and sodium dihydrogen phosphate;

[0066] Step 2, pour the solution to be separated into the feed liquid tank;

[0067] Step 3, carry out desalination operation;

[0068] Step 3.1, operate the equipment, and adjust the pressure to 1.2-1.5 MPa by the pressure regulating device, so that the solution to be separated passes through the nanofiltration membrane;

[0069] Step 3.2, collect the permeate, and return the retained liquid to the feed liquid tank;

[0070] Step 3.3, control the material concentration and keep the liquid level of the material in the feed liquid dregs, that is, add ...

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Abstract

The invention provides a method for separating and purifying 5'-cytidylic acid and derivatives thereof. The nanofiltration membrane separation technology is applied to the separation and purificationof the 5'-cytidylic acid and the derivatives thereof, and the separating and purifying process comprises desalting operation and concentrating operation. The method has the advantages of no phase change, no chemical reaction, no damage to bioactivity, no need of heating and normal-temperature operation in the separating process, simple steps, high selectivity, low energy consumption and the like.

Description

technical field [0001] The invention relates to a method for separating and purifying 5'-cytidylic acid and its derivatives. Background technique [0002] 5'-cytidylic acid can be used as raw materials, intermediates and food additives for the production of nucleotide drugs, such as for the synthesis of cytidine triphosphate (CTP), polyinosinocytes (PolyIC), aracytidine (Ara -C), raw materials of 2', 3'-dideoxycytidine (DDC), citicoline (CDP-choline) and mixed with other nucleotides as health products and food additives. [0003] In the production process of 5'-cytidylic acid, a large amount of salt can be produced, which has a greater impact on the purity of the product, especially in the recovery of the mother liquor, a large amount of salt greatly reduces the recovery rate and the quality of the recovered product, so for 5 Desalting and purification of '-cytidylic acid is crucial. [0004] The existing desalination technologies include concentrated crystallization metho...

Claims

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Application Information

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IPC IPC(8): C07H19/067C07H1/06
Inventor 苏莹杨杰瞿国良
Owner 上海新浦特种助剂有限公司
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