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Ultrasonic-assisted method for extracting total flavonoid in alfalfa

A technology for auxiliary extraction and total flavonoids, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of long extraction time, reduce equipment use efficiency, increase energy consumption, etc., and improve the extraction yield. , The effect of improving purity and extraction yield

Inactive Publication Date: 2016-07-13
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: (1) direct extraction with water increases the dissolution of impurities such as water-soluble sugars and proteins, resulting in a reduction in the purity of total flavonoids in the extract; (2) long extraction time increases energy consumption and reduces equipment efficiency of use
Another example is "Research on Microwave-Assisted Extraction of Alfalfa Flavonoids" in Volume 16, Issue 1 of "Acta Grassland Sinica", the disclosed extraction conditions are: ethanol concentration 40%, solid-liquid ratio 1:30 (g:mL), microwave power 500W Under the action of 60 seconds, under the extraction conditions, the extraction yield of flavonoids is only 4.92mg / g

Method used

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  • Ultrasonic-assisted method for extracting total flavonoid in alfalfa
  • Ultrasonic-assisted method for extracting total flavonoid in alfalfa
  • Ultrasonic-assisted method for extracting total flavonoid in alfalfa

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Ultrasonic-assisted extraction of total flavonoids from alfalfa and optimization of its condition parameters

[0046] 1. Preparation of Alfalfa Powder

[0047] The alfalfa was dried in a constant temperature drying oven at 65°C for 12 hours (the moisture content after drying was 4.74wt%), then put into a pulverizer for pulverization, and passed through a 60-mesh sieve for subsequent use. The sieved alfalfa powder was wrapped with filter paper, placed in a Soxhlet extractor, and then 15 times the volume (based on petroleum ether: sieved alfalfa powder = mL: g, the same below) of petroleum ether ( Boiling range 30-60°C), Soxhlet extraction for 24 hours to remove chlorophyll and lipids. Then the alfalfa powder was volatilized to dry petroleum ether and placed in a blast drying oven at 65° C. for 2 hours to dry (moisture content after drying was 4.7 wt %) to obtain the processed alfalfa powder.

[0048] 2. Extraction of total flavonoids from alfalfa

[0049] The ethanol ...

Embodiment 2

[0075] 1. Preparation of Alfalfa Powder

[0076] Alfalfa (aboveground part) was dried at 65°C for 12 hours in a blast drying oven (moisture content after drying was 4.25wt%), then put into a pulverizer to pulverize, and passed through a 60-mesh sieve (R40 in line with China's national standard). / 3 series) spare. Put the sieved alfalfa powder into the flask, then add 15 times the volume (mL:g) of petroleum ether (boiling range 30-60°C), and heat to reflux for 4 hours to remove chlorophyll and lipids. The mixture after the reflux is filtered, and the filter cake is washed with sherwood oil, and then the obtained alfalfa powder is evaporated to dryness of sherwood oil in a fume hood, and then placed in a blast drying oven at 65° C. for 2 hours (moisture content after drying is 4.12wt%), obtains the processed alfalfa powder.

[0077] 2. Extraction of total flavonoids from alfalfa

[0078] The ethanol solution that the volume fraction is 65% (v / v) is mixed with the alfalfa powd...

Embodiment 3

[0080] 1. Preparation of Alfalfa Powder

[0081] Alfalfa grass (aboveground part) was dried at 65°C for 12 hours in a blast drying oven (moisture content after drying was 4.18wt%), then put into a pulverizer to pulverize, and crossed a 60-mesh sieve (R40 in line with China's national standard). / 3 series) spare. Put the sieved alfalfa powder into a flask, then add 15 times the volume (mL:g) of petroleum ether (boiling range 30-60°C), and heat to reflux for 24 hours to remove chlorophyll and lipids. The mixture after the reflux is filtered, and the filter cake is washed with sherwood oil, and then the obtained alfalfa powder is evaporated to dryness of sherwood oil in a fume hood, and then placed in a blast drying oven at 65° C. for 2 hours (moisture content after drying is 4.20wt%), obtains the processed alfalfa powder.

[0082] 2. Extraction of total flavonoids from alfalfa

[0083]The ethanol solution that the volume fraction is 65% (v / v) is mixed with the alfalfa powder ...

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Abstract

The invention provides an ultrasonic-assisted method for extracting total flavonoid in alfalfa.The method comprises the following steps: (1) alfalfa is dried and smashed to obtain alfalfa powder; (2) an organic solvent (such as petroleum ether) is added to the alfalfa powder obtained in the step (1), heating reflux is performed for 4-24 hours, filtering is performed so as to remove the organic solvent, the alfalfa powder is obtained, and then the alfalfa powder is dried; (3) an ethanol solution with the volume fraction ranging from 20% to 70% and the alfalfa powder obtained in the step (1) are mixed according to the liquid-to-solid ratio of mL:g ranging from (20:1) to (70:1), extraction is performed at the temperature of 30 DEG C to 80 DEG C and under ultrasonic assistance, and an alfalfa total flavonoid extraction solution is obtained.The extraction rate of the total flavonoid can be improved with the method, the ultrasonic extraction is a physical process, the optimum temperature of the extraction process is about 60 DEG C, active ingredients of the flavonoid material in the alfalfa can be prevented from being changed by high temperature, and the extraction efficiency of the total flavonoid in the alfalfa is improved.

Description

technical field [0001] The invention relates to the technical field of plant active ingredient extraction, in particular to a method for ultrasonic-assisted extraction of total flavonoids in alfalfa. Background technique [0002] Alfalfa is a perennial herb of Leguminosae, Papilionatae Taub, Trib.trifolieaeBronn, and MedicagaL. It is widely distributed in my country, with high yield, strong adaptability, and extremely rich nutrition. Enjoying the reputation of "King of Grass", it has been valued as a good natural plant resource. Flavonoids are a class of plant secondary metabolites that are widely distributed in plants. Alfalfa flavonoids are one of the main components of alfalfa plant extracts, which have various biological effects, can effectively remove free radicals in the human body, have mild estrogen-like effects, and increase the body's fertility and immunity within a certain range . At the same time, alfalfa flavonoids can also be used as additives for functional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48
CPCA61K36/48A61K2236/10A61K2236/333
Inventor 董晓芳佟建明荆常亮
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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