Novel process for extracting golden fungus polysaccharides by step enzyme method

Abstract

The invention relates to a production process for an edible and medicinal fungus polysaccharide, belonging to the biological product production technical field, which is characterized in that a water lixiviation liquid of a golden fungus is processed step by step by the means of enzymolysis via a pectinase, a cellulose, and a neutral prolease, identifying the process conditions comprising the suitable amount, enzymolysis time, temperature, pH value and the other figures, enabling the polysaccharide component inside and outside of the golden fungus solid cell to be separated fully from the non-polysaccharide big molecules such as a pectin, a cellulose, a protein and the other members, the polysaccharide component is discharged and dissolved into the water, finally, the polysaccharide is separated from the solution by the means of ethanol sedimentation. The analysis result shows compared with the traditional water lixiviation the extraction rate of the polysaccharide is increased from 7.8% to 14.3%, the protein content in the polysaccharide is lowered from 13.3% to 1.6%, and the product yield and quality are improved drastically by utilizing the stepping enzymolysis to extract the golden fungus polysaccharide. The production process for an edible and medicinal fungus polysaccharide has the advantages of efficiently extracting the golden fungus polysaccharide with high purity, completely protecting the molecular structure and the conformation of the polysaccharide, and maintaining the biological activity under the moderate production conditions.

Description

[0001] The invention relates to a production process of edible and medicinal fungus polysaccharides, in particular to a production process of step-by-step extraction of polysaccharides by using auricularia fruit bodies as raw materials, and belongs to the technical field of biological product production. Background technique [0002] Golden ear (Tremella aurantialba), also known as golden fungus, was originally a rare wild species. It is naturally distributed in my country's Tibet, Yunnan and other places. It is a unique fungus in China. [0003] Golden ear is a kind of precious edible and medicinal fungus, which has high nutritional value. The taste of golden ear fruit is smooth and delicate. It is a rare delicacy at high-end banquets, and it is also a famous nourishing treasure that nourishes the body and mind and prolongs life. "Compendium of Materia Medica" records that "Golden Ear" is warm and cold in nature, sweet in taste, and has the effects of reducing phlegm, relievi...

AI Technical Summary

Problems solved by technology

Because golden ear is rich in protein, cellulose, pectin and other substances, it forms a dense fruiting body structure. During the water extraction process, the viscosity of the solution is extremely high, the extraction rate of polysaccharides is very low, and the extracted polysaccharides contain relatively high Too much protein, pectin, cellulose and other impurities seriously affect the purity of polysaccharides

However, extracting polysaccharides from golden ear with acid-base solution will easily destroy the active structure of polysaccharides and reduce the biological activity of polysaccharides.