HIV positive serum surrogate

A technology of positive serum and substitute, applied in the field of immune biochemistry, can solve the problem of inconclusive HIV virus complete inactivation, and achieve the effect of avoiding danger

Inactive Publication Date: 2004-04-14
SHANGHAI CRIMINAL SCI TECH RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prior art, enzyme-linked immunoimmunoassay kits are usually used for detecting antibodies against human rabies virus deficiency virus. The positive control serum of this kit is inactivated, but at present, the activity of HIV antibodies in the kit can be maintained. At the same time, the reagents The method of completely inactivating the HIV virus in the box is not conclusive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0006] ①Dissolve 8mg anti-HIV monoclonal antibody in 2ml 0.1M PH7.5 PBS, add 0.2umol SPDP with stirring at 23°C, react for 30 minutes, put in a dialysis bag, dialyze against 0.1M PH7.5PBS, change the solution four times .

[0007] ②Dissolve 16mg of two human globulins (the anti-HIV antibody activity is negative by EIA) in 2ml 0.1M PH7.5 PBS, stir and add 0.4umol SPDP at 23°C, react for 30 minutes, put in a dialysis bag, and Dialyze against 0.1M pH4.5 acetate buffer solution, and change the solution four times. Add DTT with a final concentration of 25mM at 23°C, react for 30 minutes, install a dialysis bag, dialyze against 0.1M PH7.5PBS, and change the medium four times.

[0008] ③Mix the processed products in ① and ②, react at 23°C for 1 hour, and react at 4°C for 24 hours to obtain the cross-linked product, which will be precipitated by centrifugation. One-third of meroxal antiseptic, the characteristics remain unchanged.

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Abstract

Substitute of HIV positive blood serum is cross-linking object obtained from cross-linking reaction between unhuman anti HIV antibody or its treating fluid and normal person immunoglobulin or its treating fluid. Essence of the invention is to combine unhuman anti HIV antibody activity (i.e. activity reacting to HIV antigen) with antigen activity of normal person immunoglobulin (i.e. activity reacting to the conjugate of anti abzyme of human immunoglobulin) so as to eliminate risk of containing pathogenesis factor possibly in blood serum (or blood plasma), which is positive in human anti antibody of pathogenesis factor, but possess reactivity on blood serum, which is positive in anti antibody of pathogenesis factor. Since the substitute is from unhuman anti HIV antibody and normal person immunoglobulin, thus it is essential to prevent risk infected by positive serum potentially.

Description

technical field [0001] The invention relates to the field of immunobiochemistry, in particular to a substitute for HIV positive serum. Background technique [0002] In the prior art, enzyme-linked immunoimmunoassay kits are usually used for detecting antibodies against human rabies virus deficiency virus. The positive control serum of this kit is inactivated, but at present, the activity of HIV antibodies in the kit can be maintained. At the same time, the reagents The method of completely inactivating the HIV virus in the box is not conclusive. Contents of the invention [0003] The technical problem to be solved by the present invention is: because there may be potential infection in the positive control serum in the detection kit of the human rabies disease deficiency virus antibody in the prior art, there is a potential danger for the people who need detection like this, the present invention is To solve the above-mentioned technical problems in the prior art, the ado...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/66
Inventor 曾立波
Owner SHANGHAI CRIMINAL SCI TECH RES INST
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