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Sulfonated nano fibroin-cellulose mixed film and preparation method and application thereof

A technology of sulfonated nano and silk fibers, which is applied in the field of recovery of Candida lipase, can solve the problems of loss of enzyme activity, inability to reuse, complicated process, etc., and achieve excellent reusability, good application prospects, and preparation methods simple effect

Inactive Publication Date: 2020-10-02
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the purification techniques of Candida lipase mainly include direct extraction method, ion exchange method and ultrafiltration method, but these methods have corresponding shortcomings, such as loss of enzyme activity and failure to reuse. question
[0003] Title: Immobilization of Candida rugosa lipase for the Synthesis of 6eranyl Butyrate for the Synthesis of Geranyl Butyrate Immobilization of Candida rugosa lipase for the Synthesis of 6eranyl Butyrate In the journal paper of the 11th issue of "Acta Technical Journal" in 2019, macroporous resins of different specifications were used as carriers to solidify lipase. The disadvantages are: lipase reuse rate is low, the process is complicated, it is not easy to recycle, and the pollution of chemical reagents is large, etc.

Method used

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  • Sulfonated nano fibroin-cellulose mixed film and preparation method and application thereof
  • Sulfonated nano fibroin-cellulose mixed film and preparation method and application thereof
  • Sulfonated nano fibroin-cellulose mixed film and preparation method and application thereof

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Effect test

Embodiment 1

[0044] A preparation method of sulfonated nano silk fibroin-cellulose mixed membrane and its application in reclaiming Candida lipase, such as figure 1 As shown, the specific steps are as follows:

[0045] 1) Using silk fibroin and cellulose acetate as raw materials, electrospinning technology is used to prepare nano silk fibroin-cellulose mixed membrane materials through blending;

[0046] 1-1) Degumming silkworm cocoons with sodium carbonate solution at high temperature to obtain silk fibers; the concentration range of the sodium carbonate solution is 0.2wt%, and the temperature range is 85°C;

[0047]1-2) Dissolve silk fibroin fiber in lithium bromide solution, after dialysis and filtration, take it out and freeze-dry to obtain regenerated silk fibroin; the concentration range of lithium bromide solution is 7mol L -1 , the parts by weight of silk fiber and lithium bromide solution are respectively: 1 part of silk fiber, 700 parts of lithium bromide solution;

[0048] 1-3)...

Embodiment 2

[0061] A preparation method of sulfonated nano silk fibroin-cellulose mixed membrane and its application in recycling Candida lipase, the specific steps are as follows:

[0062] 1) Using silk fibroin and cellulose acetate as raw materials, electrospinning technology is used to prepare nano silk fibroin-cellulose mixed membrane materials through blending;

[0063] 1-1) Degumming silkworm cocoons with sodium carbonate solution at high temperature to obtain silk fibers; the concentration range of the sodium carbonate solution is 0.7wt%, and the temperature range is 100°C;

[0064] 1-2) Dissolve silk fibroin fiber in lithium bromide solution, after dialysis and filtration, take it out and freeze-dry to obtain regenerated silk fibroin; the concentration range of lithium bromide solution is 9mol L -1 , the parts by weight of silk fiber and lithium bromide solution are respectively: 3 parts of silk fiber, 900 parts of lithium bromide solution;

[0065] 1-3) Dissolving regenerated si...

Embodiment 3

[0078] A preparation method of sulfonated nano silk fibroin-cellulose mixed membrane and its application in recycling Candida lipase, the specific steps are as follows:

[0079] 1) Using silk fibroin and cellulose acetate as raw materials, electrospinning technology is used to prepare nano silk fibroin-cellulose mixed membrane materials through blending;

[0080] 1-1) Degumming silkworm cocoons with sodium carbonate solution at high temperature to obtain silk fibers; the concentration range of the sodium carbonate solution is 0.5wt%, and the temperature range is 100°C;

[0081] 1-2) Dissolve silk fibroin fiber in lithium bromide solution, after dialysis and filtration, take it out and freeze-dry to obtain regenerated silk fibroin; the concentration range of lithium bromide solution is 8mol L -1 , the parts by weight of silk fiber and lithium bromide solution are respectively: 1.4 parts of silk fiber, 800 parts of lithium bromide solution;

[0082] 1-3) Dissolving regenerated ...

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Abstract

The invention discloses a sulfonated nano fibroin-cellulose mixed film and a preparation method thereof. Fibroin and cellulose acetate serve as raw materials, a nano fibroin-cellulose mixed film material is prepared through blending by using an electrostatic spinning technology, and the nano fibroin-cellulose mixed film material is chemically modified with sodium 3-sulfobenzoate to be prepared into the sulfonated nano fibroin-cellulose mixed film. According to the method, the green biomass fiber, namely fibroin fiber, and the cellulose acetate serve as the raw materials, the nano fibroin-cellulose mixed film material is prepared through blending by using the electrostatic spinning technology, then the material is chemically modified with sodium 3-sulfobenzoate to be prepared into the sulfonated nano fibroin-cellulose mixed film, the mixed film has a good adsorption effect on candida rugosa llipase, and finally the candida rugosa llipase is desorbed from the mixed film by adding neutralsalt into a solution to be recovered; and the repeated use performance of the mixed film is excellent.

Description

technical field [0001] The invention relates to the technical field of recovery of Candida lipase, in particular to a sulfonated nano silk fibroin-cellulose mixed membrane and its preparation method and application. Background technique [0002] Among the currently discovered microorganisms, nearly 60 species produce lipase, and nearly 20 are industrially produced. The action pH range, action temperature range and substrate specificity of microbial lipase are wider than those of animal and plant enzymes, which is convenient for industrial production and high-purity preparations. In recent years, microbial lipase has developed rapidly in enzyme theory research and practical application, and has been widely used in food, leather, oleochemical industries, as well as detergents, cosmetics, and medicine. my country started research on microbial lipase in the 1960s, and developed Candida lipase preparations in 1969 and supplied them to the market. Candida lipase is a hemiascospo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/728D04H1/4382D04H3/02D04H3/005D01D5/00D06M13/256D01F8/02D06M101/08D06M101/10
CPCD01D5/003D01F8/02D04H1/4382D04H1/728D04H3/005D04H3/02D06M13/256D06M2101/08D06M2101/10
Inventor 易世雄代方银孙胜童晓玲邹雨珊
Owner SOUTHWEST UNIVERSITY
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