Method for preparing propionic acid by synchronous bacterium mixing and glycerin fermentation

A technology of mixed bacteria fermentation and glycerin, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of long fermentation cycle, low propionic acid production, bacterial growth rate and low fermentation rate

Inactive Publication Date: 2010-05-12
青岛生物能源与过程研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the bacterial growth rate and fermentation rate are low, and the fermentation cycle is long
Second, product inhibition, moderate concentrations of propionic acid and acetic acid can inhibit production and lead to low propionic acid production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Preparation of seed medium

[0041] Using TY medium as the seed medium of Propionibacterium propionici, the specific composition is as follows:

[0042] Add to 1L medium: 5g tryptic soy broth, 10g yeast extract, 2.5g K 2 HPO 4 , 1.5g KH 2 PO 4 . The pH was adjusted to 7.0, and N 2 :H 2 :CO 2 =80:5:15 to maintain the anaerobic state of the medium, and sterilize at 121°C for 20 minutes. Culture medium preparation, inoculation, and sampling were all performed at an anaerobic workstation.

[0043]Using YPD medium as the seed medium of Candida rugosa, the specific composition is as follows:

[0044] Add in 1L medium: 10g yeast extract, 10g peptone, 20g glucose. The pH was adjusted to 7.0.

[0045] 2. Preparation of fermentation medium, adding glycerol to TY medium in an amount of 20g / l, pH is 7.0.

[0046] 3. Fermentation process

[0047] 1) Inoculate Propionibacterium propionici on a slant solid medium, and culture anaerobically for 72 hours at a temperature...

Embodiment 2

[0052] The preparation of the seed medium is the same as in Example 1.

[0053] The preparation of the fermentation medium is the same as in Example 1.

[0054] Put 2000ml of fermentation medium into a fermenter and sterilize at 121°C for 20 minutes. The seed liquid of above-mentioned two strains is added in the fermenter at the same time according to the total inoculum amount 10% (v / v), and the inoculation ratio is 2.5: 1 (w / w, calculate by dry cell weight), carry out anaerobic liquid state fermentation , the fermentation temperature was 30° C., and the time was 10 days. Glycerin was added twice at 96 and 168 hours during the fermentation, and 20 g of glycerol was added per 1000 ml of fermentation broth. Samples were taken every 12 hours to determine the concentration of propionic acid.

[0055] The method for detecting the concentration of propionic acid is as in Example 1. After continuous fermentation for 240 hours, the final concentration of propionic acid was 27g / l.

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PUM

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Abstract

The invention relates to a method for preparing a propionic acid by synchronous bacterium and glycerin fermentation, which comprises that: (1) facultative aerobes are inoculated onto a culture medium to carry out aerobic culture, and anaerobic bacteria are inoculated onto the culture medium to carry out anaerobic culture; (2) the facultative aerobes cultured by the step (1) are inoculated onto a seed culture medium to carry out aerobic culture, and the anaerobic bacteria cultured by the step (1) are inoculated onto the seed culture medium to carry out the anaerobic culture; and (3) seed liquids of the facultative aerobes and the anaerobic bacteria are added in a fermentation medium to carry out anaerobic liquid fermentation to prepare the propionic acid, wherein the liquid seed culture medium for carrying out the anaerobic culture in the step (2) is added with glycerin to form the fermentation medium; and the anaerobic bacteria are propionibacterium acid propionicum 1.2225, and the facultative aerobes are candida rugosa.

Description

technical field [0001] The invention relates to a method for preparing propionic acid by biological fermentation, in particular to a process flow and method for synchronous mixed bacteria fermentation of glycerin to prepare propionic acid, and a method and technology for mixed culture of two strains of bacteria. Background technique [0002] Propionic acid and its derivatives are an important class of industrial raw materials, which can be widely used in food, medicine, feed and other industries. Propionic acid and its salts such as calcium propionate, zinc propionate, and potassium propionate can effectively inhibit mold and aerobic bacillus, and are basically harmless to the human body. They are widely used in grains, feed and food, and are good antiseptic and fresh-keeping products. agent. Propionate esters are important solvents and spices, which can be used in the manufacture of butter, western wine, cold bread, cold drinks and chewing gum. Chlorine propionate and pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/52C12R1/01C12R1/72
Inventor 朱建航刘寅张永光张汝兵张帆
Owner 青岛生物能源与过程研究所
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