Preparation method and application of compound leavening agent
A compound starter and a technology for producing starter, which is applied in the fields of application, food preparation, food science, etc., can solve problems such as easy changes in the activity and proportion of bacteria, unsatisfactory food hygiene conditions, and hidden dangers in food safety, etc., to achieve The effect of shortening the production cycle, preventing the contamination of bacteria, and enriching the taste
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Embodiment 1
[0018] 1. Purification of single strains in fermented batter
[0019] Mix 50g of cooked brown rice flour, 20g of corn flour, 20g of millet flour, and 10g of soybean flour, add 200g of water at 20°C to make a batter, add 10g of flour fertilizer, and ferment for 6 hours at 30°C to obtain a naturally fermented batter; Operation Take the natural fermented paste and carry out gradient dilution to 10 -1 ~10 -6 , transferred to PDA plate medium and MRA plate medium respectively, and cultured at 30°C for 48 hours; pick the suspected yeast colonies with a colony diameter > 0.5mm on the surface of the plate and inoculate them on the PDA plate medium, and pick the lysate in the plate. The suspected colony of lactic acid bacteria with a calcium circle > 0.5mm was inoculated on the MRS plate medium, cultured at 30°C for 48 hours, and then purified by streaking on the PDA plate medium and the MRS plate medium plate for 4 generations, and the morphology was observed under a microscope. Tra...
Embodiment 2
[0045] Grind 50g of cooked brown rice flour, 10g of corn flour, 20g of wheat flour, and 20g of millet flour with 200g of water at 20°C to make batter, add 10g of flour fertilizer, and ferment for 6 hours at 30°C to obtain naturally fermented batter; according to the method of Example 1 Lactobacillus plantarum, Lactobacillus bulgaricus, Streptococcus thermophilus, Saccharomyces cerevisiae, Candida rugosa, Candida tropicalis and Pichia fermentum were isolated from naturally fermented batter as starter cultures. The number of live bacteria of each production starter strain is ≥10 9 each / ml, quick-freezing and freeze-drying the starter culture to obtain freeze-dried bacteria powder.
[0046] According to the number of parts by weight, take Lactobacillus plantarum freeze-dried bacteria powder 2g, Lactobacillus bulgaricus freeze-dried bacteria powder 1g, Streptococcus thermophilus freeze-dried bacteria powder 2g, Saccharomyces cerevisiae freeze-dried bacteria powder 3g, Candida rugo...
Embodiment 3
[0049] Grind 50g of cooked brown rice flour, 10g of corn flour, 10g of sorghum rice flour, 10g of millet flour, 10g of soybean flour, and 10g of buckwheat flour with 200g of water at 20°C to make batter, add 10g of flour fertilizer, and ferment for 6 hours at 35°C to obtain natural Fermented batter; isolate Lactobacillus plantarum, Lactobacillus bulgaricus, Streptococcus thermophilus, Saccharomyces cerevisiae, Candida rugosa, Candida tropicalis, fermented Pichia from naturally fermented batter according to the method of Example 1 Yeast is used as a starter to produce strains, and the number of live bacteria of each starter strain is ≥10 9 each / ml, quick-freezing and freeze-drying the starter culture to obtain freeze-dried bacteria powder.
[0050] According to parts by weight, take Lactobacillus plantarum freeze-dried bacteria powder 1.5g, Lactobacillus bulgaricus freeze-dried bacteria powder 1.5g, Streptococcus thermophilus freeze-dried bacteria powder 1.5g, Saccharomyces cer...
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