Method for preparing 3-hydroxypropionic acid by utilizing immobilized candida rugosa cell

A technology of Candida rugosa, hydroxypropionic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, immobilized on/in organic carriers, etc., to achieve high yields, easy control of operating procedures, and simple methods Effect

Inactive Publication Date: 2014-12-24
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the preparation of 3-hydroxypropionic acid by immobilized cells has not been reported, and further research is needed

Method used

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  • Method for preparing 3-hydroxypropionic acid by utilizing immobilized candida rugosa cell
  • Method for preparing 3-hydroxypropionic acid by utilizing immobilized candida rugosa cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of immobilized Candida rugosa cells

[0034] Candida rugosa is activated on the seed slant medium. The activation temperature is 28°C, and the activation time is 36h. The colony on the slant medium is peeled off with an inoculation loop, and the seed medium is used for cultivation. The cultivation temperature is 28°C, and the cultivation time is 36h. , then inoculate the colony on the seed medium into a 250ml Erlenmeyer flask containing 50ml of fermentation medium and shake it evenly, then culture it in a constant temperature shaking box with a rotation speed of 150r / min at 28°C for 24h to obtain liquid fermentation of Candida rugosa. Strains: filter and separate the Candida rugosa liquid fermentation strain and the fermentation medium with sterile gauze, wash the Candida rugosa liquid fermentation strain with deionized water, and follow the Candida rugosa liquid fermentation strain: Sodium alginate solution=1:1 (w / v) ratio, take 0.1g strain, add 100ml s...

Embodiment 2

[0042] (1) Preparation of immobilized Candida rugosa cells

[0043] Candida rugosa is activated on the seed slant medium. The activation temperature is 30°C and the activation time is 48h. The colony on the slant medium is peeled off with an inoculation loop, and the seed medium is used for cultivation. The cultivation temperature is 30°C and the cultivation time is 30h , then inoculate the colony on the seed medium into a 250ml Erlenmeyer flask containing 60ml of fermentation medium and shake it evenly, then cultivate it in a constant temperature shaking box with a rotation speed of 200r / min at 30°C for 30h to obtain liquid fermentation of Candida rugosa. Strains: filter and separate the Candida rugosa liquid fermentation strain and the fermentation medium with sterile gauze, wash the Candida rugosa liquid fermentation strain with deionized water, and follow the Candida rugosa liquid fermentation strain: Sodium alginate solution=1.5:1 (w / v) ratio, take 0.3g strain, add 200ml ...

Embodiment 3

[0051] (1) Preparation of immobilized Candida rugosa cells

[0052] Candida rugosa is activated on the seed slant medium. The activation temperature is 32°C, and the activation time is 60h. The colony on the slant medium is peeled off with an inoculation loop, and the seed medium is used for cultivation. The cultivation temperature is 32°C, and the cultivation time is 48h. , then inoculate the colony on the seed medium into a 250ml Erlenmeyer flask containing 80ml of fermentation medium and shake it evenly, then culture it in a constant temperature shaking box with a rotation speed of 250r / min at 32°C for 48h to obtain liquid fermentation of Candida rugosa. Strains: filter and separate the Candida rugosa liquid fermentation strain and the fermentation medium with sterile gauze, wash the Candida rugosa liquid fermentation strain with deionized water, and follow the Candida rugosa liquid fermentation strain: Sodium alginate solution=2:1 (w / v) ratio, take 0.4g strain, add 200ml s...

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Abstract

The invention provides a method for preparing 3-hydroxypropionic acid by utilizing an immobilized candida rugosa cell. The method comprises the following steps: (1) preparing the immobilized candida rugosa cell; (2) inoculating the immobilized candida rugosa cell to a fermentation medium for shake cultivation; (3) filtering and separating the immobilized candida rugosa cell and the fermentation medium by sterile gauze after the cultivation is completed, adding a fresh fermentation medium with the same volume as the fermentation medium in the step (2) and culturing under the same condition as the step (2); (4) repeating the step (3), and terminating cultivation when the immobilized candida rugosa cell is completely ruptured; and (5) determining the content of 3-hydroxypropionic acid in the fermentation medium which is filtered and separated each time by utilizing high performance liquid chromatography. The preparation method is simple and convenient, has easily controlled operation program and high yield, is capable of overcoming the defect that a traditional non-immobilized strain and the fermentation medium are difficult to separate, and has the advantages of recycling, reducing cost and the like.

Description

technical field [0001] The invention relates to the field of preparation of 3-hydroxypropionic acid, in particular to a method for preparing 3-hydroxypropionic acid by using immobilized Candida rugosa cells. Background technique [0002] 3-hydroxypropionic acid (3-hp) is an important chemical intermediate that has emerged in recent years. It can be used as a raw material for the production of malonic acid, 1,3-propanediol, succinic acid, special polyester and acrylic acid. Prepare a variety of other important fine chemical products. It is an important bio-based compound with strategic significance and one of the effective ways to solve the resource and energy crisis. Especially in my country, a large number of agricultural waste bio-based resources have not been developed every year. Utilization not only wastes resources, but also causes environmental pollution. [0003] At present, developed countries in the world have realized the industrialization of bio-based conversion 3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12N11/10C12N11/04C12R1/72
Inventor 高明侠王陶董玉玮张传丽
Owner XUZHOU UNIV OF TECH
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