Cellulose degradation bacteria agent and raw material strain, and preparation methods and application of cellulose degradation bacteria agent and raw material strain

A technology for degrading cellulose and bacterial strains, which is applied in the preparation of organic fertilizers, biochemical equipment and methods, and applications. The effect of enhanced synergy

Active Publication Date: 2014-09-24
KWEICHOW MOUTAI COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although vinasse contains a large amount of cellulose, as disclosed in CN1928074A, CN101560488A, CN101974436A and CN1460664A, the effect of being applied to vinasse composting is not ideal

Method used

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  • Cellulose degradation bacteria agent and raw material strain, and preparation methods and application of cellulose degradation bacteria agent and raw material strain
  • Cellulose degradation bacteria agent and raw material strain, and preparation methods and application of cellulose degradation bacteria agent and raw material strain
  • Cellulose degradation bacteria agent and raw material strain, and preparation methods and application of cellulose degradation bacteria agent and raw material strain

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Experimental program
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Effect test

Embodiment 1

[0038] The Paecilomyces varioti MM2 strain, the Aspergillus fumigatus MM6 strain, the Candida rugosa MJ4 strain and the Bacillus licheniformis MX8 strain were respectively placed in a K2HPO42g, (NH4)2SO41.4g, MgSO4.7H2O0.3g, CaC120.3g, CoC122mg, FeSO4.7H2O5mg, MnSO4.H2O1.6mg, ZnSO41.7mg, agar 20g, distilled water 1000mL. pH 7.2-7.3, in the slant culture vessel of rice straw powder and bran 3% straw bran liquid medium, by at 28 ℃, liquid culture 2d, obtain Paecilomyces varioti (Paecilomyces varioti) MM2 bacterial strain respectively, Seed culture solution of Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain; then transfer the seed culture solution to the On the bran, the mold was cultured at 28°C and the bacteria were cultured at 37°C for 9 days to obtain Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa (Candida rugosa) MJ4 bacterial strain and Bacillus licheniformis (Bacillus licheniformis) MX8 b...

Embodiment 2

[0040] The Paecilomyces varioti MM2 strain, the Aspergillus fumigatus MM6 strain, the Candida rugosa MJ4 strain and the Bacillus licheniformis MX8 strain were respectively placed in a NaNO32.5g; KH2PO41g; CaCl2 2H2O0.1g; MgSO40.3g; NaCl0.1g; After culturing for 9 days, the seed culture solutions of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain were respectively obtained Then the seed culture solution was transferred to the bran sterilized at 121° C. for 30 minutes, and the mold was cultivated at 28° C. and the bacteria at 37° C. for 9 days to obtain Paecilomyces varioti MM2 bacterial strain respectively. , Aspergillus fumigatus (Aspergillus fumigatus) MM6 strain, Candida rugosa (Candida rugosa) MJ4 strain and Bacillus licheniformis (Bacillus licheniformis) MX8 strain single strain body, and then the single strain body was air-dried to obtain a solid microbial agent; 2 kg of Paecilomyces vario...

Embodiment 3

[0042] The Paecilomyces varioti MM2 strain, the Aspergillus fumigatus MM6 strain, the Candida rugosa MJ4 strain and the Bacillus licheniformis MX8 strain were respectively placed in a Potato 100g / L, glucose 20g / L, agar 20g / L, pH7.2~7.4, in the slant culture vessel of PDA liquid medium prepared with distilled water, through liquid culture at 30 ℃ for 6 days, respectively to obtain Wan's pseudo Penicillium (Paecilomyces varioti) MM2 strain, Aspergillus fumigatus (Aspergillus fumigatus) MM6 strain, Candida rugosa (Candida rugosa) MJ4 strain and Bacillus licheniformis (Bacillus licheniformis) MX8 strain seed culture liquid; After receiving the bran sterilized at 121°C for 30 minutes, the mold was cultured at 28°C and the bacteria at 37°C for 9 days to obtain Paecilomyces varioti MM2 strain and Aspergillus fumigatus MM6 strain respectively. strain, Candida rugosa (Candida rugosa) MJ4 strain and Bacillus licheniformis (Bacillus licheniformis) MX8 strain single strain body, and then ...

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PUM

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Abstract

The invention relates to the technical field of a biological agent, and particularly relates to a cellulose degradation bacteria agent and a raw material strain, and preparation methods and applications of the cellulose degradation bacteria agent and raw material strain. The compound bacteria agent for degrading cellulose mainly comprises a paecilomyces varioti MM2 strain, an aspergillus fumigates MM6 strain, a candida rugosa MJ4 strain, and a bacillus licheniformis MX8 strain. The cellulose can be efficiently degraded and utilized by the compound bacteria agent disclosed by the invention, the compound bacteria agent can be used as a detonating agent and an enhancer of wine sediment compost, the compost process can be accelerated, the compost rotten degree is improved, an organic fertilizer is fabricated, and the cellulose degradation bacteria agent is especially applicable to resourceful treatment of lignocelluloses solid waste and production of the organic fertilizer by using the wine sediment.

Description

technical field [0001] The invention relates to the technical field of biological bacterial agents, in particular to a cellulose-degrading bacterial agent, a raw material strain, a preparation method and an application thereof. Background technique [0002] Composting technology is a technical means to make organic fertilizer through microbial fermentation and decomposing organic solid waste under high temperature and high humidity conditions. In the composting process of lignocellulosic organic solid waste, the degradation of cellulose is the key, because the cellulose content is the highest and the degradation is the slowest. In aerobic composting, it is difficult to effectively degrade the cellulose in the compost material only by relying on the original indigenous microorganisms in the compost material. Therefore, it is necessary to introduce specific exogenous microorganisms to accelerate the compost decomposition process. For the bacterial agent that degrades cellulos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F17/00C12R1/79C12R1/68C12R1/72C12R1/10
CPCY02W30/40
Inventor 王莉汪地强王和玉江友峰胡旭袁颉王岩
Owner KWEICHOW MOUTAI COMPANY
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