Decapsulated artemia egg preparation method improving hatching rate

A technology for artemia eggs and hatching rate, applied in the field of Artemia eggs, can solve the problems of affecting hatching rate, low operation difficulty, increase of solution temperature, etc., and achieves the effects of stable shelling effect, simple operation steps and improved hatching rate

Active Publication Date: 2017-04-26
江苏好润生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: chemical methods are often used when the existing artemia eggs are shelled, and the reaction cannot be terminated immediately after shelling, and the residual chlorine on the surface of the shelled eggs continues to act on the shelled eggs, making the shelled eggs Membrane damage is relatively large, and the temperature of the solution increases during shelling, which affects the hatching rate. A kind of organic acid is provided to soften the egg shell, and biological enzymes and bile are compounded to decompose and dissolve the egg shell to shell Artemia eggs to prepare shelling. The method for shelling Artemia eggs, the present invention first uses hawthorn as a raw material to squeeze the juice, and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] First, weigh 100g of immature hawthorn, after manually removing the seeds, wash it with deionized water for 3 times, put the washed hawthorn and 500mL of deionized water in a juice extractor to obtain hawthorn juice; Add 100g of Artemia eggs into the beaker, then add 300mL of 8% sodium chloride solution and 200mL of the above-mentioned hawthorn juice, aerate for 3 hours to make the Artemia eggs fully absorb water and swell, then filter with gauze to collect Artemia eggs, Obtain the pretreatment Artemia ovum; Then get 300g fresh pig bile and 2g phospholipase C and join in the beaker that fills 300mL deionized water, stir and mix after 3min, add above-mentioned pretreatment Artemia ovum again, and pass into air, control The air flow is 50m 3 / (m 2 min), soak for 30 minutes to decompose the phospholipids and cholesterol in the Artemia egg shells, then filter with gauze to collect the Artemia eggs for later use; then weigh 10 parts of olive oil and 20 parts of peptone acco...

example 2

[0020] First, weigh 130g of immature hawthorn, after manually removing the seeds, wash it with deionized water for 4 times, put the washed hawthorn and 550mL of deionized water in a juice extractor to obtain hawthorn juice; Add 150g of Artemia eggs into the beaker, then add 350mL of 8% sodium chloride solution and 250mL of the above-mentioned hawthorn juice, aerate for 3 hours to make the Artemia eggs fully absorb water and swell, then filter with gauze to collect Artemia eggs, Obtain pretreatment Artemia ovum; Then get 350g fresh pig bile and 3g phospholipase C and join in the beaker that fills 350mL deionized water, after stirring and mixing for 4min, add above-mentioned pretreatment Artemia ovum again, and pass into air, control The air flow is 60m 3 / (m 2min), soaked for 35 minutes to decompose the phospholipids and cholesterol in the Artemia egg shells, then filtered through gauze to collect the Artemia eggs for later use; then weighed 15 parts of olive oil and 25 parts ...

example 3

[0023] First, weigh 150g of immature hawthorn, after manual de-seeding, wash 5 times with deionized water, put the washed hawthorn and 600mL deionized water in a juice extractor together to obtain hawthorn juice; Add 200g of Artemia eggs into the beaker, then add 400mL of 8% sodium chloride solution and 300mL of the above-mentioned hawthorn juice, aerate for 4 hours to make the Artemia eggs fully absorb water and swell, then filter with gauze to collect Artemia eggs, Obtain pretreatment Artemia ovum; Then get 400g fresh pig bile and 4g phospholipase C and join in the beaker that fills 400mL deionized water, after stirring and mixing for 5min, add above-mentioned pretreatment Artemia ovum again, and pass into air, control The air flow is 70m 3 / (m 2 min), soak for 40 minutes to decompose the phospholipids and cholesterol in the Artemia egg shells, then filter with gauze to collect the Artemia eggs for later use; then weigh 20 parts of olive oil and 30 parts of peptone accordin...

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Abstract

The invention relates to a Decapsulated artemia egg preparation method improving the hatching rate, and belongs to the technical field of artemia eggs. The decapsulated artemia egg preparation method improving the hatching rate includes the steps: taking haw as the raw material, juicing the haw, and performing dydration on artemia eggs by means of the haw juice with organic acidity and light salt brine; and by coordination of bile and phosphatidase C, decomposing the phosphatide and cholesterol in the eggshells of the artemia eggs, and by coordination of Candida rugosa bacterium and the lipoprotein lipase and active substance secreted from the Candida rugosa bacterium, and papain, decapsulating the artemia eggs, wherein the egg envelopes of the decapsulated eggs have high resistance to papain and lipoprotein lipase so that the reaction can be terminated even no special inhibitor is used and the decapsulated artemia eggs can be obtained. Through the decapsulated artemia egg preparation method improving the hatching rate, the hatching rate of the obtained decapsulated artemia eggs is greatly improved when the decapsulated artemia eggs are hatched, and achieves 93-96%. Moreover, the decapsulated artemia egg preparation method improving the hatching rate is stable in the decapsulating effect and is low in the operating difficulty.

Description

technical field [0001] The invention relates to a method for preparing shelled artemia eggs for improving hatchability, and belongs to the technical field of artemia eggs. Background technique [0002] Artemia, also known as Artemia, is a small beetle distributed worldwide, living in high-saline waters such as salt lakes and salt pans. Artemia eggs are high-quality bait for aquatic fry and play an important role in aquaculture. Artemia eggs are iron-containing lipoproteins, which cannot be digested by young fish and shrimp. Therefore, it is generally necessary to hatch Artemia larvae first, and then feed the larvae as bait. The hatched egg shells and larvae are not easy to be completely separated, and it is easy to cause part of the hatched larvae to be lost with the shells, or the egg shells and larvae are eaten together, resulting in the death of fish and shrimp seedlings due to intestinal obstruction. Artemia eggs can be fed directly after shelling, and larvae can also ...

Claims

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Application Information

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IPC IPC(8): A01K61/20
Inventor 汪逸凡孟中立
Owner 江苏好润生物科技有限公司
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